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Vascular bundle tissue-specific expression promoter, vector containing it, transformant and application thereof

A tissue-specific, promoter-based technology, applied in the field of plant genetic engineering, can solve problems such as plant death, economic loss, and shedding

Active Publication Date: 2021-07-27
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cotton verticillium wilt is a fungus that infects the roots of cotton, gradually causing the leaves of cotton to turn yellow and then fall off. In severe cases, it will further cause the death of the plant. Vascular bundle diseases seriously affect the quality of cotton and cause serious economic losses.

Method used

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  • Vascular bundle tissue-specific expression promoter, vector containing it, transformant and application thereof
  • Vascular bundle tissue-specific expression promoter, vector containing it, transformant and application thereof
  • Vascular bundle tissue-specific expression promoter, vector containing it, transformant and application thereof

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Embodiment 1

[0032]本实施例提供一种维管束组织特异性表达启动子,所述启动子由甜菜心基因组中获取,其核苷酸序列如SEQ ID:No 1所示:tctctcaggggtaaaaggcttaaaaagcaaagattaaaagaaagattactttctagtagaaagtttctattgttaccgccatcgacaacaacaaaaattatgtggccaagttgggccaagtcttgggtttgactctggttagtggtcccattactttgtgtatgtgaatgtgatggttttaacttttaactagcactcttctatgataaaaatcctttgatgctccttattgtataatatgtgttcgagtttacagaggtgagtatcgcggcttgtaatagttatgttagtttggtggccaaatcaaactccttaaggagttagacattacaaaaagcaaggaacggaagcaataaagaaactcaatctctaaatagccatcaaaattgattagatgaagtcaattttgtaaacaacgtctaggtgactagaaagctaagaattaaatgaatcggtagcttgaattttggtgagataacatatgttgctctctcgccatagttcataattaaatggtgatcatatataggtctggtctcaagcctacgccagtaaaggacataaagccaagcatgggaaggtggcacagctagcaccttcagtaatgcacgttaatagacttttggttatattttaggagtattatttacaagtattaatgaatgtaagtaaatgatattttatattttctatacaacttcaaaataagtttggattttatatttctttgaacaaaaaacaattctgaaactatttcacataatatgatgtttattcccctaatactttcacatttagcctcatttagaggaatctatctaaccttaccaaactaataaggaaaaaaaactgtaaaaaacattgaaatgaggaatcacacatggatttccattctgtaatcttgtactttggattctccactataatttg...

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Abstract

The invention discloses a vector, a transformant and an application thereof comprising a vascular bundle tissue-specific expression promoter. The promoter is obtained from the sugar beet heart genome, and its nucleotide sequence is shown in SEQ ID: No 1. The promoter can significantly promote the expression of the reporter gene GUS in the vascular tissue of Arabidopsis, and it has a specific expression effect in the vascular tissue of the plant, so that the disease resistance gene driven by the promoter can be expressed in the vascular tissue of the plant. It can be expressed in the medium to resist Fusarium wilt and other pathogens, which provides a new way for plant disease-resistant breeding.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, in particular to a pBrpHMA2 promoter specifically expressed in vascular bundle tissue and its application. Background technique [0002] Gene expression is regulated by different factors, among which transcriptional regulation is an important regulatory step of gene expression. Cis-acting elements and trans-acting factors work together to regulate gene transcription and expression, and promoter is an important cis-acting element that plays an important role in the specific expression of genes. Promoters are divided into three categories according to their mode of action: constitutive promoters, inducible promoters and tissue-specific promoters. [0003] So far, a large number of tissue- or organ-specific promoters have been isolated, and related studies have shown that the Arabidopsis HMA2 gene is mainly driven by the vascular tissue of roots, stems and leaves of plants. The e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/10C12N15/82A01H5/00A01H6/20
CPCC07K14/415C12N15/8223
Inventor 唐玉林邓艳午刘帅
Owner SHENZHEN UNIV
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