Method for extracting and separating high-viscosity narcissus polysaccharides from narcissus bulbs

[0046] Embodiment one: the preparation of narcissus bulb polysaccharide

[0047] (1) Take fresh narcissus bulbs, peel off the outer skin, cut off the root hairs, and rinse with clean water;

[0048] (2) Take 200 g of narcissus bulbs, add 400 mL of 0.05% potassium sorbate+0.1% EDTA aqueous solution, and beat with a homogenizer;

[0049] (3) Add 1600mL aqueous solution containing 0.05% potassium sorbate + 0.1% EDTA to the homogenate, and stir slowly for 30 minutes;

[0050] (4) filter with 200 mesh nylon cloth, collect filtrate;

[0051] (5) Adjust the pH to 3-4 with trichloroacetic acid, and stir at 15r / min for 30min;

[0052] (6) Centrifuge at 2000G for 30min, collect the supernatant;

[0053] (7) Adjust the pH of the supernatant to about 6 with alkali, and then concentrate it to 3-5 ° brix with a rotary evaporator;

[0054] (8) Add 3 times of alcohol to the concentrated solution, stir slowly, let it stand for 60 minutes, filter and collect the precipitate with 100 mesh ny...

[0056] Embodiment two: the preparation of narcissus bulb polysaccharide

[0057] (1) Take fresh narcissus bulbs, peel off the outer skin, cut off the root hairs, and rinse with clean water;

[0058] (2) Get 2 kg of narcissus bulbs, shred them, and put them into extraction tanks;

[0059] (3) Add 10kg containing 0.05% potassium sorbate+0.1% EDTA aqueous solution in the extraction tank, and stir for 120min, discharge; Leach again 2 times, each solvent is 5kg;

[0060] (4) Combine the extracts, filter with 200 mesh nylon cloth, and collect the filtrate;

[0061](5) Adjust the pH to 3-4 with trichloroacetic acid, and stir at 30r / min for 30min;

[0062] (6) Centrifuge with a tubular centrifuge to collect the light phase;

[0063] (7) Adjust the pH of the light phase to about 6 with alkali, and then concentrate it to 3-5 ° brix with a falling film evaporator;

[0064] (8) Add 3 times of alcohol to the concentrated solution, stir slowly for 90 minutes, filter and collect the prec...

[0066] Embodiment three: the preparation of narcissus bulb polysaccharide

[0067] (1) Take fresh narcissus bulbs, peel off the outer skin, cut off the root hairs, and rinse with clean water;

[0068] (2) get narcissus bulb 100kg, cross extruder, drop into extraction tank;

[0069] (3) Add 1000kg containing 0.05% potassium sorbate+0.1% EDTA aqueous solution to the leaching tank, and stir for 120min, discharge; leaching again 1 time, the solvent is 800kg;

[0070] (4) Combine the extracts, filter with a filter bag of 200 mesh to 400 mesh, and collect the filtrate;

[0071] (5) Adjust the pH to 3-4 with citric acid, and stir at 60r / min for 30min;

[0072] (6) Centrifuge with a tubular centrifuge to collect the light phase;

[0073] (7) Adjust the pH of the light phase to about 6 with alkali, and then concentrate it to 3-5 ° brix with a falling film evaporator;

[0074] (8) Add 2.5 times of alcohol to the concentrated solution, stir slowly for 120 minutes, then filter and col...