Growth-promoting composite endophytic bacterial agent and application thereof

[0031] In the present invention, the preparation method of the solid bacterial powder preferably includes the following steps: after activating the strain, carry out continuous two-stage seed liquid culture, insert into the fermentation medium for fermentation, and concentrate to obtain concentrated bacterial liquid; The concentrated bacterial solution of Enterobacter aerogenes CT-B09-2 and Acinetobacter calcoaceticus WYS-A01-1 was mixed with a protective agent (skimmed milk powder) and then freeze-dried to obtain the solid bacterial powder; the Bacillus amyloliquefaciens The fermentation liquid of JL-B05 and bacillus amyloliquefaciens JL-B06 is mixed with light calcium carbonate and then spray-dried to obtain the solid bacteria powder.

[0032] In the present invention, the strains are inoculated in NB medium and cultured for 15-25 hours to obtain a primary seed solution; the culture temperature is preferably 25-35°C, and the culture speed is preferably 150-350 r min -1 .

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[0038] (1) Preparation of Enterobacter aerogenes CT-B09-2 bacterial liquid and solid bacterial powder:

[0039] Preparation of primary seed solution of Enterobacter aerogenes CT-B09-2

[0040] Activate Enterobacter aerogenes CT-B09-2 strain, pick a single colony, inoculate in 150ml NB medium (sterilize for 30min at 121°C), 28°C, 180 r min -1 Under the condition of shaking culture for 20 h, the primary seed solution was obtained.

[0041] Preparation of Secondary Seed Solution of Enterobacter aerogenes CT-B09-2

[0042] Put the first-grade seed solution into a sterile seed tank, the inoculum amount is 0.5% ~ 1.0% (V / V); the culture conditions are: manually adjust the speed and air flow of ventilation to adjust DO (DO40%), and the culture temperature is 25℃~ 35℃, the speed is 200~350r·min -1 , tank pressure 0.05MPa; cultivation time 10~15 h. OD of secondary seed solution 600 The value is between 0.6 and 0.8, at this time the strain is the strongest.

[0043] Preparation o...

[0064] The growth-promoting composite endophytic agent liquid preparation 1 prepared in Example 1 and Enterobacter aerogenes CT-B09-2, Acinetobacter calcium acetate WYS-A01-1, Bacillus amyloliquefaciens JL-B05 and Bacillus amyloliquefaciens JL - B06 single-species fermented mature bacterial liquid, respectively prepared with sterile water so that the content of viable bacteria is 1×10 8 The cfu / mL bacterial suspension was used to inoculate the moso bamboo seedlings (30mL / plant) through root irrigation, and then use the LI-6400 portable photosynthesis tester produced by the American LI-COR company to carry out the cleaning of the leaves of the moso bamboo seedlings on 15d and 30d. Photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO 2 Determination of photosynthesis indicators such as concentration.

[0065] The net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and intercellular CO of bamboo leaves were measured 15 d...