Marker screening identification and application of non-concentrated reduced apple juice based on non-targeted metabonomics

[0078] 1. Screening and verification of identification markers of non-concentrated reduced apple juice and concentrated reduced apple juice

[0079] 1. Sample collection and preparation

[0080] Non-concentrated reduced apple juice and concentrated apple juice samples were collected from typical areas such as Shaanxi and Shandong in my country. The sources included a factory in Shandong (recorded as group A) and three factories in Shaanxi (recorded as groups B, C and D). Comprehensive consideration Variety, place of origin, batch, sterilization method (pasteurization and ultra-high pressure non-thermal sterilization), etc.; each factory has no less than 18 samples of each kind of apple juice. NFC apple juice samples included pasteurized apple juice and ultra-autoclaved apple juice. Among them, the pasteurized NFC apple juice of group A was directly provided by the factory, and the pasteurized NFC apple juice of groups B, C and D were processed at 85°C for 15 seconds in the lab...

[0105] Based on the 21 kinds of marker molecules of Example 1, the PLS-DA model for constructing and distinguishing NFC apple juice and FC apple juice is established, which includes:

[0106] Get 199 parts of apple juice samples in Example 1 and centrifuge (26916g, 4 ℃) 15min in centrifuge, get supernatant afterwards and filter in the PES membrane filter tip of 0.45 and 0.22 μ m, then pack into sampling bottle, as Samples were used for subsequent analysis.

[0107] Data acquisition was performed using an ultra-high performance liquid chromatography tandem quadrupole time-of-flight high-resolution mass spectrometer. The specific parameters of the chromatographic system are as follows: All samples were analyzed using ExionLC ultra-high performance reversed-phase liquid chromatography system (SCIEX, Redwood City, CA, USA), mobile phase A was 0.2% formic acid aqueous solution, B was acetonitrile, and the flow rate was 300 μL / min; The injection volume was 2 μL; the chromatographic...

[0114] The PLS-DA model of Example 2 is used for actual identification of the samples to be tested.

[0115] Adopt the PLS-DA model of the identification NFC apple juice of embodiment 2 and FC apple juice to identify the apple juice sample to be identified, the method is as follows:

[0116] (1) Take the 71 apple juice samples not used in Example 2 as the apple juice samples to be identified, centrifuge in a centrifuge (26916g, 4°C) for 15min, then take the supernatant and put them in 0.45μm and 0.22μm PES Membrane filter head filter, then put into the sample bottle, as a sample for subsequent analysis.

[0117] Data acquisition was performed using an ultra-high performance liquid chromatography tandem quadrupole time-of-flight high-resolution mass spectrometer. The specific parameters of the chromatographic system are as follows: All samples were analyzed using ExionLC ultra-high performance reversed-phase liquid chromatography system (SCIEX, Redwood City, CA, USA), mobile p...