Fibronectin-coated tannic acid/iron complex drug-loaded nano material and preparation and application thereof

[0078] (1) Weigh 3.48mg of doxorubicin hydrochloride and dissolve it in methanol (1mL), add triethylamine to deprotonate, wherein the molar ratio of doxorubicin hydrochloride to triethylamine is 1:1, after fully reacting for 4h Afterwards, hydrophobic doxorubicin (DOX) was obtained; the methanol solution of DOX was mixed with tannic acid (TA) (0.2mg / mL) dissolved in absolute ethanol and stirred, and ferric chloride (FeCl 3 ) aqueous solution (0.5mg / mL), the above mixed solution was stirred for 12h overnight, the methanol and ethanol were volatilized and removed, and centrifuged at 13000rpm at 4°C for 30min to obtain the drug-loaded nanomaterial of the tannic acid / iron complex loaded with doxorubicin (DOX-TAF), where TA / FeCl 3 The molar ratio of / DOX is 1:1:2.

[0079] (2) The drug-loaded nanomaterial (DOX-TAF) (1 mg) of doxorubicin-loaded tannic acid / iron complex was dissolved in ultrapure water (1.5 mL), and fibronectin solution (0.1 mg / mL, 5mL ultrapure water), mixed with ...

[0081] Take 1 mg of TAF synthesized in Comparative Example 1, DOX-TAF and DOX-TAF@FN synthesized in Example 1, and dilute each group of materials to 50 μg / mL with ultrapure water for the determination of surface potential and hydrodynamics diameter. As shown in Table 1, the potential of TAF was −24.5 mV and that of DOX-TAF was −18.2 mV, and the potential increased, and the particle size increased to 190.1 nm, demonstrating the successful loading of DOX. After bovine serum albumin is coated on the surface of DOX-TAF by physical extrusion, the potential drops to -29.2mV, and the hydrated particle size becomes 232.2nm; and after fibronectin is coated with hydrogen bonds, the potential becomes - 25.5mV, the hydrated particle size changed to 221.3nm, which proved the successful coating of fibronectin on the DOX-TAF surface. The hydrodynamic diameter of DOX-TAF@FN in water, PBS solution and RPMI 1640 medium can remain basically unchanged for a long time ( figure 2 ), proving that ...

[0085] Take TAF, DOX-TAF, DOX-TAF@FN prepared in Example 1 for ultraviolet characterization, as image 3 As shown, in the curves b and c, the absorption peak at 480nm is the characteristic peak of DOX, which proves the successful loading of DOX.