Novel goose astrovirus and duck tembusu virus multiplex fluorescent quantitative PCR detection primer probe set, kit and application

[0044]In the present invention, primers and probes are successively designed for the E gene of Tambusu virus (DTMUV), the ORF1b gene, ORF1a gene, and ORF2 gene of novel goose star virus (GoAstV), as shown in Table 1 and Table 2, a total of 9 For primers and probes. The amplification effects of each pair of primers and probes in Table 2 are shown in turn Figure 10-Figure 14 As shown, there is no amplified signal. However, only the combination of primers and probes designed for the E gene of Tambusu virus (DTMUV) and the ORF1b gene of novel goose Astrovirus (GoAstV) (Table 1) had better effects. The primers and probes in Table 1 are described in detail below, as are the experimental schemes and experimental effects.

[0045] Table 1 Successful primer information

[0046]

[0047] Table 2 Failed primer information

[0048]

[0049] The fluorescent quantitative PCR experiment was carried out according to the instructions of the kit Nanjing Nuoweizan AceQ qPCR Probe Mast...