Nucleic acid metabolic enzyme activity detection method

[0037] Single base extension error assay (capillary electrophoresis assay)

[0038] Design and synthesis of primers and templates:

[0039] Single-stranded nucleic acid sequence (5'-3'):

[0040] GACCGCGACTCCAGCCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAGTCGTCGCGGGATCA (SEQ ID NO. 1)

[0041] Primer sequence (5'-3'):

[0042] TGATCCCGCGACGACT (SEQ ID NO.2)

[0043] TGATCCCGCGACGACTTT (SEQ ID NO.3)

[0044] TGATCCCGCGACGACTTTG (SEQ ID NO.4)

[0045] TGATCCCGCGACGACTTTGAATT (SEQ ID NO. 5)

[0046]It is used to detect the reaction ability of different nucleic acid metabolizing enzymes and different fluorescently modified nucleotide derivatives, adding a general template and one of four different fluorescently labeled nucleotides (wrong nucleotides that do not match the template) into the reaction system Within 1 min, for the extension reaction, start the reaction by adding the nucleic acid metabolizing enzyme whose activity is to be detected to the...