Fecal biomarker for diagnosing or preventing diabetes mellitus, detection reagent and application of fecal biomarker

[0053] (1) Experiments were carried out with 6-8 week-old healthy mice, and the mice were divided into four experimental groups, with 3 mice in each group, namely: normal control group (Con), low-dose radiation group (LDR), high-dose radiation group (LDR), Sugar-high-fat diet group (HFD), high-sugar and high-fat diet combined with low-dose radiation group (LDR+HFD); the normal control group was not treated, and the mice in the low-dose radiation group were irradiated with cobalt-60 rays once a week at a dose of 0.05Gy, continuous irradiation for 10 weeks, the high-sugar and high-fat diet group was fed with a high-sugar and high-fat diet, and the high-sugar and high-fat diet combined with low-dose radiation group was fed with a high-sugar and high-fat diet and irradiated once a week with cobalt-60 rays at a dose of 0.05Gy, continuous irradiation for 10 weeks. The mice were reared for 3 months.

[0054] (2) Collect feces samples from mice in the four experimental groups, add 90...

[0062] This example studies the effects of fecal flora transplantation on HFD (high-sugar and high-fat diet group), LDR+HFD (high-sugar and high-fat diet combined with low-dose radiation group) mice on insulin sensitivity, proline (PA) metabolism and Effect of PYCR1 expression. The mice were transplanted with fecal suspension of healthy control group, HFD case and LDR+HFD case, which were defined as Trans-Con, Trans-HFD and Trans-HFD+LDR, respectively. Image 6 Shown is a timeline of fecal bacterial gavage of mice depleted of gut microbiota antibiotics, in which 5 mice per group were treated with antibiotics in drinking water for 4 days and suspended twice a week with feces. The mice were gavaged with liquid, and tested on the 25th day after the intestinal microbiota antibiotics were depleted. Figure 7-14 The results of blood glucose level, glucose tolerance, insulin tolerance, insulin level, insulin resistance index, fasting blood glucose value, proline level, and lipopolys...