Activatable nano photosensitizer as well as preparation method and application thereof

[0041] A preparation method of an activatable nanometer photosensitizer, comprising the following steps:

[0042] Under sonication, MDA probe (50 μg) and distearoylphosphatidylethanolamine-polyethylene glycol (5 mg) were added to tetrahydrofuran (THF), the components were mixed well, and quickly poured into deionized water. A large excess of distearoylphosphatidylethanolamine-polyethylene glycol can wrap the MDA probe as much as possible and avoid the damage of the molecular probe. After 10-15 minutes of continuous sonication, the hydrophobic MDA probe can enter the hydrophobic cavity of the amphiphilic polymer distearoylphosphatidylethanolamine-PEG, and the hydrophilic layer of the polyethylene glycol can be exposed on the particles. surface, and then the solution was evaporated by a rotary evaporator at 40-50 °C to remove excess THF. Finally, the nanophotosensitizer solution is purified 3-5 times by ultrafiltration to remove the unsuccessfully modified MDA probe, and finall...