Preparation method and application of hippophae rhamnoides fruit extract for inhibiting xanthine oxidase

[0055] The invention provides a preparation method of a sea buckthorn extract with xanthine oxidase inhibitory activity. The specific preparation steps are as follows:

[0056] (1), the dried sea buckthorn fruit utilizes 80% ethanol extraction, suction filtration, collects the filtrate, and the filtrate is concentrated under reduced pressure to obtain the sea buckthorn ethanol extract; Extraction in turn to obtain petroleum ether extraction part (HF-PF), ethyl acetate extraction part (HF-EF) and n-butanol extraction part (HF-BF) respectively;

[0057] (2), the seabuckthorn ethyl acetate extraction part is separated by silica gel column, and uses CH 2 Cl 2 / MeOH stepwise elution yields six components Fr1-Fr7;

[0058] (3), use petroleum ether / ethyl acetate to separate Fr5 on a silica gel column to obtain eight components Fr5a to Fr5h; wherein Fr5g is purified by SephadexLH-20, eluted by reverse-phase ODS gradient, and then obtained by semi-preparative HPLC compound 1;

[00...

[0071] (1) The dried sea buckthorn fruit (10kg) was extracted with 3×20L of 80% ethanol at 80°C for 2h, the extract was filtered and concentrated under reduced pressure to obtain an ethanol extract; the extract was diluted with distilled water (5L), followed by Extract with petroleum ether (PE, 3×4.5L), ethyl acetate (EA, 3×4.5L) and n-butanol (n-Bu, 3×4.5L) to obtain 150 g of petroleum ether extract fraction (PF), respectively, Ethyl acetate extract fraction (EF) 80g and n-butanol extract fraction (BF) 1022g;

[0072] (2), Seabuckthorn ethyl acetate extract was separated by silica gel column (100-200 mesh), and used CH 2 Cl 2 / MeOH stepwise elution (100 / 1→50 / 1→20 / 1→10 / →8 / 10→6 / 1→4 / 1→2 / 1→1 / 1→MA) yields seven components (Fr 1-Fr7); take 2 mg of the three extracts respectively, dissolve with a small amount of DMSO, and then dilute to 10 mL with buffer to obtain 200 μg·mL -1 The seven components were tested for xanthine oxidase activity. The results are shown in Table 1. The ac...