Systhesizing of chimeric peptide from anteron promoted by human's fine hair

[0029] Example of hCG Chimeric Peptide CP1

[0030] Materials and methods

[0031] 1. Restriction enzymes EcoR I, Sal I, Nde I, and BamH I are products of Boehringer Mannheim in the United States, T4 DNA ligase is a product of British Biolabs, T4 polynucleotide kinase is a product of New England Biolabs in the United States, and Taq I DNA polymerase It is a product of the State Key Laboratory of Genetic Engineering of Fudan University, IPTG is a product of Promega in the United States, protein low molecular weight standards and alkaline phosphatase-linked goat anti-mouse secondary antibody (IgG / HRP) are products of Huamei Bioengineering Company, 5-bromo-4- Chloro-3 indole-β-D-galactoside (X-gal) and isopropylthio-β-D-galactoside (IPTG) are products of Sigma, USA, which recognize hCGβ 133-139 The sequenced monoclonal antibody OT3A is a product of OrganonTechnika in the Netherlands.

[0032] 2. Cloning and sequencing The vector pBluescript KS (PBS) was purchased from Stratagen...