Recombined expression plamid vector, expression antige of N gene of bubble cell stomatitis virus and preparation method
A technology for vesicular stomatitis and genetic recombination, applied in the fields of viral antigen components, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of inability to express nucleoproteins efficiently, achieve rapid and sensitive regulation, overcome operational Heavy, highly sensitive effects
Inactive Publication Date: 2005-01-12
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Problems solved by technology
For the nucleoprotein gene of the VS virus, it has been reported that a eukaryotic expression system is used, and a few reports suggest that the prokaryotic expression system cannot express the nucleoprotein efficiently
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[0029] (1), construction of VSV N gene recombinant plasmid
[0030] Cloning and sequence analysis of vesicular stomatitis virus nucleoprotein gene by Hua Qunyi et al., Chinese Journal of Biological Products, No. 1, 2004, pages 4 to 7, cloning of vesicular stomatitis virus N gene And sequencing, construction of VSVN gene recombinant plasmid, the recombinant plasmid was named pMD-VN5.
[0031] (2), construction of VSV N gene recombinant expression vector
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This invention relates to a diagnostic reagent used in preparing VSV N protein recombination antigen and its preparation method. The reagent includes its N gene recombination expression plasmid vector and its antigen. The preparation method includes 1) cloning the VSV coding group specific antigen N gene fragment to pMD18-T plasmid vectors to make up of N gene clone recombination plasmid, 2) sub-cloning plugging into the pBAD / Thio TOPO expression vector, 3, converting TOP10 cells, 4) screening positive clones getting SVS N reading code frame.
Description
technical field [0001] The invention relates to a biological preparation for preparing virus diagnostic reagents, especially a biological preparation capable of detecting vesicular stomatitis virus and a preparation method of the biological preparation. Background technique [0002] Vesicular stomatitis (VS) is a major zoonotic disease caused by vesicular stomatitis virus (VSV) belonging to the Rhabdoviridae family Vesiculovirus. The virus is divided into Indiana (Indiana, IN type) and New Jersey (New Jersey, NJ type) two serotypes. Acute febrile flu-like symptoms appear after infection. Cattle, pigs, horses, sheep and many other wild animals can be infected, and the clinical symptoms are not easy to distinguish from Foot and Mouth disease (FMD). The International Office of Epizootics (OIE) listed the disease as a class A infectious disease of animals. It is an important quarantine object in international animal trade. [0003] The commonly used diagnostic methods for VS...
Claims
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IPC IPC(8): A61K39/12C12N1/21C12N15/47C12N15/66C12N15/70C12Q1/68G01N33/53
Inventor 花群义徐自忠董俊杨晶焰杨云庆周晓黎贾建军肖荣海龙忠保
Owner CHECKOUT & QUARANTINE TECH CENT YUNNAN ENTRY &EXIT CHECKOUT & QUARANTINE BUR
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