Method for assaying sulfaquinoxaline and special enzyme-linked immune reagent kit

[0036] Example 1. Preparation and detection method of an enzyme-linked immunosorbent assay kit using a conjugate of a sulfaquinoxaline hapten and a carrier protein as a coating source.

[0037] The ELISA kit using the conjugate of sulfaquinoxaline hapten and carrier protein as the coating source includes:

[0038] (1) a microtiter plate coated with sulfaquinoxaline and carrier protein conjugate;

[0039] (2) Alkaline phosphatase-labeled goat anti-mouse anti-antibody working solution: Dilute the alkaline phosphatase-labeled goat anti-mouse anti-antibody to a protein concentration of 0.5 μg / L. The diluent used for the enzyme marker working solution contains 0.05% glycerin (which can prevent the enzyme marker from being placed in the -20°C environment from freezing, and can also maintain the biological activity of the enzyme marker for a long time), 1% thimerosal preservative (convenient save) solution.

[0040] (3) sulfaquinoxaline standard substance solution: with the pH valu...

[0091] Example 2. ELISA kit using goat anti-rabbit anti-antibody as coating source and its preparation method

[0092] The ELISA kit with goat anti-rabbit anti-antibody as the coating source includes:

[0093] (1) ELISA plate coated with goat anti-rabbit anti-antibody;

[0094] (2) Alkaline phosphatase-labeled sulfaquinoxaline hapten working solution: dilute the alkaline phosphatase-labeled sulfaquinoxaline hapten to a protein concentration of 0.5 μg / L. The diluent used for the enzyme marker working solution contains 0.05% glycerin (which can prevent the enzyme marker from being placed in the -20°C environment from freezing, and can also maintain the biological activity of the enzyme marker for a long time), 1% thimerosal preservative (convenient save) solution.

[0095] (3) Sulfaquinoxaline standard solution: 6 bottles of sulfaquinoxaline series standard solutions, 0μg / L, 1μg / L, 3μg / L, 9μg / L, 27μg / L, 81μg / L, 3ml / bottle.

[0096] (4) Chromogenic solution: 4-nitrophenol phos...

[0118] Embodiment 3, test kit precision, accuracy and shelf life test

[0119] 1. Kit precision test

[0120] (1) Precision test of standard product

[0121] Three batches of the kits prepared in Example 1 and Example 2 were taken respectively for precision experiments, and 10 kits were extracted from each batch of kits, and 20 microwells were respectively extracted from the enzyme-linked plate of each kit. Measure the absorbance value (OD value) of the 9 μg / L standard solution, and calculate the coefficient of variation. The measurement results of the three batches of kits in Example 1 are shown in Table 1, and the results show that the coefficient of variation ranges from 5.1% to 11.9%.

[0122] 1

2

3

4

5

6

7

8

9

10

CV%

01 batch

6.5

7.4

9.1

10.8

5.1

8.4

7.5

6.4

6.2

10.9

03 batches

11.9

8.5

6.4

7.5

9.4...