Multi-index protein chip inspection reagent unit of cardiovascular disease diagnosis and prediction

[0036] Preparation of detection reaction plate

[0037]Take a 48-well plate, set 42 sample wells and 6 standard wells. Place a solid phase support (NC membrane or PDVF membrane) at the bottom of each reaction well, and the solid phase support is coated with anti-CRP, anti-Myoglobin, anti-cTnI, anti-cTnT, anti-NT-proBNP, anti-CK-MB , protein microarrays of eight antibodies including anti-FABP and anti-GPBB ( figure 1 ).

[0039] Kit preparation

[0040] Prepare a test kit containing eight indicators of cardiovascular disease integrated detection, which contains a reaction well plate (24-48 people) of embodiment 1, 1 bottle of washing concentrate, 1 bottle of enzyme-labeled working solution, and 1 bottle of detection solution A , a bottle of detection solution B, and a standard bottle.

[0041] Washing Concentrate, Enzyme Label Working Solution, Detection Solution A, Detection Solution B

[0043] Integrated detection of eight indicators of cardiovascular disease

[0044] Test each blood sample according to the following method:

[0045] 1. Add test blood or standard substance to the corresponding reaction well, 75ul / well.

[0046] 2. Add enzyme-labeled working solution, 75ul / well.

[0047] 3. Incubate and shake at 37°C for 60 minutes.

[0048] 4. Wash 4-6 times with lotion, 2 minutes each time, vibrate.

[0049] 5. Add detection solution 20ul / well, so that the detection solution is evenly distributed at the bottom of the well.

[0050] 6. CCD detection, exposure for 30-60 seconds depending on the strength of the luminescent signal.

[0051] Result judgment:

[0052] According to the CutOff value of different indicators, refer to the gradient curve of the standard to determine the negative or positive of the sample serum.

[0053] At the same time, the serum samples tested were routinely tested by conventional methods. The result shows that the test result...