ABO blood group inverse reagent

[0143] Preservation solution preparation:

[0144] Trisodium citrate 14.705g

[0145] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.408 g

[0146] Disodium hydrogen phosphate (Na 2 HPO 4 )0.716g

[0147] Disodium edetate 1.0 g

[0148] Sodium chloride (NaCl) 8.182 grams

[0149] Glucose monohydrate (Glucose) 19.810g

[0150] Adenine 0.405g

[0151] Inosine 2.682g

[0152] Chloramphenicol 0.66g

[0153] The above components were adjusted to 2 liters with distilled water.

[0154] preparation:

[0155] The above components were mixed and dissolved in distilled water to a constant volume, and 0.45 μl of a stainless steel high-pressure filter membrane was used to sterilize at 121° C. with 0.15 Mpa high-pressure steam for 30 minutes.

[0156] Blood type erythrocytes that have been accurately identified as blood type A are added to the above preservation solution for preparation, and the concentration of blood type erythrocytes is 3%.

[0157] Blood type erythrocytes w...

[0161] Preservation solution preparation:

[0162] Trisodium Citrate 10g

[0163] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.3 g

[0164] Disodium hydrogen phosphate (Na 2 HPO 4 ) 0.5 g

[0165] Disodium edetate 0.8 g

[0166] Sodium chloride (NaCl) 6 grams

[0167] Glucose monohydrate (Glucose) 15 grams

[0168] Adenine 0.3g

[0169] Inosine 2 grams

[0170] Chloramphenicol 0.5g

[0171] The above components were adjusted to 2 liters with distilled water.

[0172] preparation:

[0173] The above components were mixed and dissolved in distilled water to a constant volume, and 0.45 μl of a stainless steel high-pressure filter membrane was used to sterilize at 121° C. with 0.15 Mpa high-pressure steam for 30 minutes.

[0174] Blood type erythrocytes that have been accurately identified as blood type A are added to the above preservation solution for preparation, and the concentration of blood type erythrocytes is 3%.

[0175] Blood type erythrocytes with accuratel...

[0179] Preservation solution preparation:

[0180] Trisodium Citrate 20g

[0181] Potassium dihydrogen phosphate (KH 2 PO 4 ) 0.5 g

[0182] Disodium hydrogen phosphate (Na 2 HPO 4 ) 0.9 g

[0183] Disodium edetate 1.2g

[0184] Sodium chloride (NaCl) 10 grams

[0185] Glucose monohydrate (Glucose) 25 grams

[0186] Adenine 0.5g

[0187] Inosine 3 grams

[0188] Chloramphenicol 0.8g

[0189] The above components were adjusted to 2 liters with distilled water.

[0190] preparation:

[0191] The above components were mixed and dissolved in distilled water to a constant volume, and 0.45 μl of a stainless steel high-pressure filter membrane was used to sterilize at 121° C. with 0.15 Mpa high-pressure steam for 30 minutes.

[0192] Blood type erythrocytes that have been accurately identified as blood type A are added to the above preservation solution for preparation, and the concentration of blood type erythrocytes is 3%.

[0193] Blood type erythrocytes with accurat...