Protein and small molecule polypeptide for increasing lysozyme bacteriolysis activity and its use

[0013] 1. Binding of TRAP protein to lysozyme and activation of lysozyme activity

[0014] 1.1 Materials

[0015] TRAP protein was expressed and purified by our laboratory, and lysozyme was purchased from SIGMA. Polyclonal antibodies to TRAP and lysozyme were prepared by our laboratory. HRP-labeled anti-rabbit secondary antibody and lysozyme activity kit were purchased from Laibo Company.

[0016] 1.2 Method

[0017] 1.2.1 ELISA method to detect the combination of TRAP protein and lysozyme

[0018] ① Coat the ELISA plate with TRAP protein at a coating concentration of (2 μg / well), overnight at 4°C, wash once with PBST, block with 10% fetal bovine serum at 37°C for 1 hour, wash with PBST three times, add lysozyme (5 μg / well) Wells), incubated at 37°C for 1 hour, washed three times with PBST, added lysozyme antibody (1:1000), incubated at 37°C for 1 hour, washed three times with PBST, added HRP-labeled anti-rabbit secondary antibody (1:10000), TMB Substrate chromogenic solu...