Measuring apparatus and method for determining the degree of bacterial contamination of process liquids
a technology of process liquid and measuring apparatus, which is applied in the direction of instruments, biomass after-treatment, specific use bioreactors/fermenters, etc., can solve the problems of environmental loads, loss of quality in production, and the above-mentioned methods are too slow
Active Publication Date: 2021-06-15
ADVATEC PROJECTS GMBH & CO KG
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Benefits of technology
The invention relates to a measuring apparatus and method that allows for online monitoring of bacterial contamination in process liquids. This apparatus and method can provide a reliable signal value in a short time (a few hours) and can detect bacteria in almost all liquid media encountered in industrial baths. The apparatus includes a cylindrical, bell-shaped, or funnel-shaped gas collecting hollow body that can significantly boost the sensor signal and detection limits. The apparatus can also include agitators for homogenization or expulsion of process liquids or gas produced by bacteria. The gas concentration is measured by a gas sensor, which evaluates the degree of bacterial contamination in the process liquid and can trigger the automatic addition of a biocide to the liquid based on the measured bacterial concentration.
Problems solved by technology
In the exponential growth phase (duplication rates ˜100 min) of the bacteria the above-mentioned methods are too slow.
Biocides cannot always be admetered in good time, so that losses of quality occur in production.
This is very expensive and additionally loads the environment.
This conceals very significant disadvantages and risks in robust use in industrial process baths.
On the one hand, in the case of aggressive media the attachments (lines, pumps, valves, etc.) can be easily damaged so that the service life thereof is very short.
On the other hand, due to deposits and bacterial contaminations of the measuring facility the results can be substantially falsified.
Washing devices can help here, but are very cost-intensive not only in procurement, but also in maintenance.
In the case of CDC coatings (CDC=cathodic dip coating) such attachments are almost impossible, since this process liquid has a tendency to coagulation and sedimentation.
This method has the disadvantage that dead cells and contaminating particles are also included in the count.
Moreover, sample removal with subsequent spectroscopic evaluation of the ATP content in a laboratory is necessary, so that this cannot be regarded as an ‘on-line method’.
Method used
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[0027]The measuring apparatus 10 substantially consists of a bell 15, also termed gas collecting hollow body, a sensor device 20 with a gas sensor array 12 with at least one gas sensor, a gas pump 14 and an evaluating unit 13. The evaluating unit 13 can have an interface 25 with respect to a plant control 26. The bell 15 preferably has the form of funnel, but it can also have the form of a cylinder or other geometric hollow body. As considered in its intended use setting the bell 15 has an upper, preferably cylinder-shaped or cylindrical, neck 27. The neck 27 transitions in downward direction into a downwardly widening and downwardly open funnel 28. The funnel 28 has a conical funnel wall 29. At its lower edge the funnel 28 has an encircling, preferably circular, edge 30.
[0028]For measuring operation, the funnel-shaped bell 15 optionally together with the agitator 24 is transferred by means of a controllable raising and lowering device 22, which comprises an ultrasonic sensor, or ma...
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Abstract
A measuring apparatus and a method for determining the degree of bacterial contamination of process liquids uses at least one gas sensor for measuring the gas concentration of a gas producible by aerobic bacteria in the process liquid. An evaluating device is connected with the sensor for evaluating a sensor signal generated by the sensor and correlated with the degree of bacterial contamination. To determine the degree of bacterial contamination a funnel-shaped gas collecting bell is partly immersed in the process liquid so that a gas collection cavity for collection of the escaping gas is formed directly above the process liquid surface in the gas collecting bell. The gas escaping can be fed by a gas pump via a gas feed line to the sensor, conducted via the sensor and pumped back again to the gas collection cavity by way of a gas return line.
Description
CROSS REFERENCE TO RELATED APPLICATIONS[0001]Applicant claims priority under 35 U.S.C. § 119 of German Application No. 10 2018 102 658.7 filed Feb. 6, 2018, the disclosure of which is incorporated by reference.BACKGROUND OF THE INVENTION1. Field of the Invention[0002]The invention relates to measuring apparatus and a method for determining the degree of bacterial contamination of process liquids.2. Description of the Related Art[0003]Increasingly higher numbers of bacteria appear in the process baths of current industrial installations. In order to determine the bacterial count culture media are incubated with process liquid and subsequently incubated in an incubator. After approximately two days the cell count is determined by counting the cell colonies that have grown. This procedure is necessary in order to ensure process stability. In the case of increased bacteria count values of >104 CFU / ml (CFU=‘colony-forming units’) substantial disruptions to process can occur, which usu...
Claims
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Patent Timeline
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IPC IPC(8): C12M1/34G01N1/24C12Q1/04C12Q3/00C12Q1/06G01N1/28C12M1/00B01L5/02G01N33/00
CPCG01N1/24C12Q1/04C12Q1/06C12Q3/00G01N1/286B01L5/02C12M29/24C12M41/34G01N33/004
Inventor MAUS, MICHAELWIMMER, THOMASBERNER, ULRICHJOSUPEIT, FRANK
Owner ADVATEC PROJECTS GMBH & CO KG
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