Presented are methods and apparatus for delivering a 
surgical instrument to a treatment site within the body of a subject, enabling accurate placement of surgical tools in areas not directly visible to a surgeon during a surgical procedure, while reducing or eliminating need for real-time 
imaging modalities to guide placement of those surgical tools. A treatment tool is guided to a treatment site by placing a guiding element at a 
reference site within a body of a subject, the 
reference site having a known 
spatial relationship to the treatment site, and utilizing a positioning tool to guide a treatment tool to a locus so positioned with respect to that guiding element that the 
spatial relationship between that guiding element and that locus is substantially similar to the 
spatial relationship known to exist between the 
reference site and the treatment site, thereby positioning the treatment tool substantially at the treatment site. Methods and apparatus for focusing energy at a treatment site, methods and apparatus for treatment of 
Benign Prostate Hyperplasia, and methods and apparatus for performing a saturation 
biopsy of an organ are also presented.