The invention provides a kit, primers, probe sequences and a method for detecting 
fetus chromosome aneuploid. The kit comprises the multiple pairs of 
specific primers and a plurality of specific 
Taqman probes all of which are designed according to 13#, 18# and 21# chromogene sequences of the human, and further comprises a 
reaction system used for digital PCR, wherein the 
nucleotide sequence of the 
specific primers and the specific 
Taqman probes is SEQ ID NO: 1-90. The detection method comprises the steps that firstly, the 
specific primers and the specific 
Taqman probes are designed according to the 13#, 18# and 21# chromogene sequences of the human; secondly, 
peripheral blood of a (12-23)-week pregnant woman is collected for 
plasma DNA extracting; thirdly, the 
reaction system of the digital PCR amplification 
gene sequence is prepared; fourthly, the numbers of 13#, 18# and 21# chromosomes of a 
fetus are calculated through digital PCR detection for the number of the chromosomes, digital PCR is used for detecting FAM and VIC 
fluorescence of all reaction units of a 
chip, and the copy number of the 13# 
chromosome, the copy number of the 18# 
chromosome and the copy number of the 21# chromosome are calculated respectively. By means of the kit and the method, the number of the 13# chromosome, the number of the 18# chromosome and the number of the 21# chromosome of the 
fetus can be efficiently, quickly and accurately detected without invasion, and the kit and the method are beneficial to clinic application and popularization.