The invention relates to a preparation method of a spatially staggered mixed material film and an application thereof in an achromatic 
superlens. The method comprises the following steps that firstly, a multi-sector spatial deposition 
mask is processed by using a thin 
silicon wafer, partitioned covering is carried out by using the multi-sector 
mask, and a first 
dielectric material film is deposited on a super lens substrate; after the 
mask and redundant materials are removed through an 
etching technology, another multi-sector mask is replaced, and a second 
dielectric material film with the same thickness is deposited on the remaining sectors; and finally, a nano structure of the 
superlens is processed through micro-nano technologies such as 
electron beam 
lithography and the like. The invention provides a new thought for preparing the high-efficiency dual-
wavelength achromatic 
superlens and applying the high-efficiency dual-
wavelength achromatic superlens to two-
photon microscopic imaging and two-
photon STED super-resolution micro 
endoscopic imaging, the superlens realizes high-efficiency 
confocal of 
infrared band exciting light and 
visible band fluorescent light or loss light, therefore, the method is expected to be used in super-resolution micro 
endoscopic imaging of deep biological tissues of living bodies, and dynamic real-time super-resolution imaging with low invasion damage and 
high resolution is further realized.