The invention relates to a display substrate, a preparation method thereof and a 
display device, and belongs to the technical field of display. The display substrate comprises a substrate, and dividedinto a display area and a non display area positioned in the periphery of the display area, the display area includes multiple pixel areas, each pixel area is provided with a pixel structure, each pixel structure comprises organic and inorganic film 
layers arranged in a stacked way, the region, close to the edge, of the non display area serves as an anti-
cracking reinforcement region which is only provided with the 
organic film layer, and the 
organic film layer at least covers the outer surface of the inorganic film layer adjacent to the anti-
cracking reinforcement region. Thus, the inorganicfilm layer is fundamentally prevented from a crack caused by an outside force, crack transfer is eliminated, and further, the framework is narrowed, the anti-
impact performance of the display substrate is improved, the 
cutting energy is reduced, the 
cutting yield rate is improved, and the 
cutting effect is improved.