The invention discloses a method for establishing a 
bladder cancer in-situ model of a 
nude mouse. The method is characterized by comprising the following steps of: narcotizing a female 
nude mouse superficially, and inserting a 
urethral catheter with a side hole into a bladder from a urethral canal, so that negative pressure is formed in the 
urethral catheter continuously; penetrating a cutter into the 
urethral catheter, and adjusting the positions of a 
cutting inclined plane and the side hole of the urethral 
catheter; 
cutting under the negative pressure to 
cut part of 
mucous membrane layers off, so that the lower layer of a bladder 
mucous membrane is exposed to form a local 
wound surface; and pouring 
human bladder cancer cell suspension which is in the logarithm growth period into a 
bladder cavity of the 
nude mouse and reserving for a period of time, discharging the 
cell suspension out, and establishing the superficial 
bladder cancer model after 7 days. Compared with the conventional electrocauterization method, the conventional chemical medicinal 
corrosion method and the conventional bladder puncture intracavity 
cutting method, the method has the advantages that molding assemblies are low in production cost, and the method for establishing the 
bladder cancer model is good in effect, the device is easy to operate, the time is short, the nude mouse has little pain, and death caused by 
anesthesia, operation strike and infection is reduced obviously.