The invention discloses a mmethod for obtaining analogous 
retinal tissues rich in cone and rod cells by using 
human induced pluripotent stem cells. The method comprises the following steps: digestinghiPSCs to obtain 
cell pellets, and then carrying out suspension cultivation on the 
cell pellets to obtain an 
embryoid body; reinoculating the 
embryoid body into a culture dish enveloped by 
Matrigel, and carrying out induced differentiation inside an induced cultivation solution to obtain nerve 
retina (NR) and RPE; stirring up the NR and the RPE, carrying out suspension cultivation to obtain 3D analogous 
retina comprising NR tissues, then carrying out continuous suspension cultivation inside an optimized culture solution without 
retinoic acid, carrying out NR differentiation on all 
retinal cells, comprising highly matured photoreceptor cells, 
Rhodopsin positive rod cells, L / M 
OPSIN positive red and green cone cells and S-
OPSIN positive blue cone cells, and thus particularly obtaining the analogous 
retinal tissues rich in red and green cone and rod cells.