The present invention discloses a preparation method for a 
chitosan-based 
porous scaffold with 
biological activity. According to the present invention, 
chitosan and inorganic micro / nanoparticles with biological activities are adopted as raw materials to prepare into 
slurry; a water-soluble 
aldehyde polysaccharide polymer is adopted as a cross-linking agent; a 
Schiff base cross-linking process and a 
freeze drying method are adopted to prepare a class of 
chitosan-based composite 
porous scaffold materials with biological activities, wherein porosities, pore sizes, mechanical properties, degradation performances, and bone conductions / bone inductions and the like can be regulated through the formula and the 
process conditions. According to the present invention, the 
porous scaffold provided by the present invention does not adopt harmful chemical cross-linking agents, and can not cause adverse effects to cells; with the cross-linked porous structure and a plurality of hydrophilic groups such as amino, carboxyl, hydroxyl and the like, the porous 
scaffold has high 
chemical stability in an aqueous phase with a wide 
pH range (
pH range of 5-9), and has outstanding advantages of softness, high elasticity, 
swelling ratio of 30 times, and the like; the porous 
scaffold has advantages of 
imitation of 
natural bone extracellular microenvironment in aspects of the porous structure, the 
chemical composition, the surface 
chemistry and the like.