105results about How to "Promotes Adhesive Growth" patented technology

The invention relates to a bracket material used for an osseous tissue project, and a preparation method thereof. Firstly, the bracket of a type I collagen is extracted from a small fresh pigskin by a mature extracting technique. Then, the bracket is further expanded in a Tris cushion liquid, the pH of which is equal to 8.8 to obtain a natural porous collagen bracket by the treatments of freezing and drying. The bracket is respectively and repeatedly mineralized in a CaCl2 liquid and in (NH4)2HPO4 liquid or mineralized in simulated body fluid for a long period to lead the weakly crystallized HA to be uniformly settled into the collagen bracket; and then a pigskin collagen-hydroxyapatite ossein is obtained by the treatments of freezing and drying to replace the natural bracket material. The invention not only maintains the natural bracket structure of the collagen in an organism, but also has the advantages of low material cost, simple devices, short period and easy operation. The obtained compound bracket material used for the pigskin collagen-hydroxyapatite osseous tissue project has the characteristics of high intensity, large toughness, non-antigenicity, higher biological activity as well as degradation and releasing control.

The invention discloses an electrostatic spinning nanofiber-extracellular matrix composite material as well as a preparation method and application thereof. The preparation method is simple and feasible in steps, and large-sized equipment is not needed. The prepared composite material has the advantages of electrostatic spinning nanofibers and an extracellular matrix. Compared with a pure electrostatic spinning nanofiber material or a pure extracellular matrix, the composite material has very high biocompatibility and very high mechanical property, is easier for cell adhesion and proliferation, has very good application prospect and high practical value in mechanical tissue engineering repair, and can be used for effectively promoting the adhesive growth, proliferation, migration and differentiation and amplifying and culturing stem cells on a large scale. Moreover, the composition material is low in antigenicity, and the risk of disease transmission is eliminated by means of strict screening.

The invention discloses biological ink material for 3D printing and a preparation method and application thereof. The biological ink material is prepared by, by mass concentration percentage, 5-18% of biological macromolecules, 76-93.5% of water or 76-93.5% of pre-crosslinking agent, and 1.5-6% of coagulant, wherein the sum of the mass concentration percentage of the above raw materials is 100%. The biological ink material is characterized in that water washing and crosslinking are performing for shaping after printing. The biological ink material has the advantages that the biological ink material can be easily squeezed out during printing, does not block printing needles and can be easily shaped after being squeezed out; the biological ink material is low in antigenicity in the bodies of organisms, low in caused rejection reaction, biodegradable, nontoxic to the organisms and high in safety.

The invention discloses a three-dimensional single cell culture chip realized based on a micro-fabrication technology, and belongs to bio micro electro mechanical systems (Bio-MEMS). The chip comprises cavities distributed in an array; adjacent cavities are connected with each other through a micro groove; the depth of the micro groove is consistent with the height of the cavities; and the bottoms of the cavities are provided with nano projections. According to difference of adhesion of cells on the surface of blank polydimethylsiloxane (PDMS) and on the surface of PDMS with a nano micro morphology structure and by combining a surface topology pattern, the chip realizes cell patterning culture. The chip can be used for cell culture without chemical or biological reagents, overcomes a defect of low stability of the conventional patterning method, has the characteristics of durable and stable pattern, controllable preparation process and the like, and can meet the requirement of batch production. Meanwhile, a patterning design that the cavities are connected through micro grooves is adopted, and the research on the interaction of cells can be realized.

The invention discloses a chitosan / polylysine in-situ gel and a preparation method thereof. The gel is an in-situ gel prepared from 1-5.5 percent by weight of sulfhydrylization chitosan and 0.1- 3.2 percent by weight of maleimide polylysine. The preparation method comprises the following steps of synthetizing the polylysine containing maleimide double bonds, and preparing a maleimide polylysine solution by taking a PBS (phosphate buffer solution) as a solvent; preparing a sulfhydrylization chitosan solution by taking the PBS as a solvent; and uniformly mixing the two solutions under the condition of physiological pH value to obtain the in-situ gel. The invention has the advantages that the preparation process is simple, and the prepared in-situ gel simulates the components, the structure and the functional characteristics of polysaccharide / polypeptide of a natural extracellular substrate, helps to increase histocompatibility of a material, promotes cell adhesion growth and has wide application prospects in the fields of tissue engineering and drug release.

The invention relates to a preparation method of porous fiber with a controllable degradation rate for a tissue engineering scaffold, which comprises the following steps: uniformly mixing a biodegradable polymer and a pore-forming agent, vacuum drying, melt spinning, drawing, immersing into hydrochloric acid or distilled water to remove the pore-forming agent, and vacuum drying, thereby obtainingthe porous fiber with micro pores with the diameters of 10-100mum uniformly distributed on the surface thereof. The preparation method of the invention is simple and is applicable to industrial production. The diameters of the micro pores of the prepared fiber are matched with cell sizes, so that cells can be easily adhered into the micro pores on the surface of the fiber for growth. Simultaneously, the degradation rate is controllable.

The present invention discloses an injectable-porous-drug loaded polymethyl methacrylate-based composite scaffold bone transplant material and a preparation method thereof, and belongs to the field of organic functional materials preparation. The polymethyl methacrylate-based composite scaffold bone transplant material uses polymethyl methacrylate (PMMA) as a scaffold for providing mechanical supporting, and a chitosan-based thermosensitive hydrogel as a pore forming agent and an osteoconductive material and a drug carrier, and the polymethyl methacrylate (PMMA) and the chitosan-based thermosensitive hydrogel are mixed with each other to form an injectable-porous three-dimensional structural bone cement composite. The scaffold bone transplant material is simple in preparation method, suitable in reaction temperature, and good in biocompatibility, has matched mechanical properties, good biological mineralization and corresponding anti-bacterial, anti-inflammatory or anti-tumor capabilities, and has broad prospects in clinical application of reconstruction of bone tissues in future.

The present invention relates to a method for preparing porous ceramics by using organic foamed microspheres as pore-forming agent and adopting hot press moulding process, and said method includes the following steps: firstly, utilizing paraffin melt solution to soak the treated organic foamed microspheres, then uniformly mixing the ceramic powder body and biological glass adhesive, hot press moulding, dewaxing, removing plastics and sintering treatment so as to obtain the invented product. The described organic foamed microsphers are polystyrene foam spheres, polyurethane sphere and polyvinyl chloride foam spheres. Said invention has the features of high porosity, three-dimensional communication and high specific surface area.

The invention discloses a cell culture plate based on oriented polymer nanometer fiber and a preparation method thereof. The cell culture plate comprises a standard flat-bottom cell culture plate used as a main body and a polymer nanometer fiber support adhered on the hole bottom of the culture plate. The polymer nanometer fiber support is prepared by adding bioactive components into the main raw material--polymer, carrying out grafting or blending, then carrying out electrostatic spinning and carrying out collection by using a fiber collecting device of a parallel conductive panel; and fiber bundles on the polymer nanometer fiber support are of a regular oriented arrangement structure. The culture plate provided by the invention has good biocompatibility and is applicable to culture of cells or tissue with oriented morphological characteristics, especially to culture and regeneration of cells and related tissue of nerves, bones, muscles, ligaments, etc.

The invention discloses a composite artificial cornea and a preparation method thereof. The composite artificial cornea comprises a central optical pillar prepared from polyhydroxyethyl methacrylate (PHEMA), a corneal skirt structure prepared from an acellular stromal hydrogel, and an intermediate sandwich grid scaffold connecting the central optical pillar and the corneal skirt structure, whereinthe corneal skirt structure is provided with a nerve cell scaffold layer to which cells are adhered and a corneal stromal cell scaffold layer. The composite artificial cornea is simple in structure,good in biocompatibility, easy to process, can well simulate the environment of cornea in vivo, and is favorable for transplantation.

Provided is a cervical pedicle screw feeding guiding formwork. The formwork surface of the guiding formwork is a profiling faying surface of a spinous process area on a single section cervical vertebra; the back side of the faying surface of the guiding formwork is provided with a handle; corresponding to a spinous process bump of the spinous process area, a limiting through hole penetrates the handle and the guiding formwork, a limiting tail pin is movably installed in the limiting through hole, and the tip of the limiting tail pin is embedded into and penetrates the spinous process bump; corresponding to pedicles on the two sides of the spinous process area, bosses are protruded on the back side of the faying surface of the guiding formwork respectively, the bosses are provided with hollow access through holes, and sleeves are movably installed in the hollow access through holes. The sleeves are medical resin sleeves. The guiding formwork has a sufficient faying area and faying stability, the shaking of the guiding formwork relative to a vertebral plate during an operation is effectively prevented, the pin feeding accuracy can be guaranteed for different patients, and the operation difficulty and the operation risk are lowered. The medical resin sleeves are high in consistency degree with the human tissue, pin feeding guiding for double-side or single-side vertebral body guiding can be achieved.

The invention discloses wound dressing composite nano fiber membrane, prepared from polyvinylidene fluoride, a pH-responding macromolecular polymer and collagen. A preparation method of the wound dressing composite nano fiber membrane comprises the steps of 1) preparing a shell solution, to be specific, adding polyvinylidene fluoride and the pH-responding macromolecular polymer into hexafluoroisopropanol, stirring well to obtain the shell solution; 2) preparing a core solution, to be specific, dissolving collagen in hexafluoroisopropanol, adding drugs, and stirring well to obtain the core solution; 3) injecting the shell solution of step 1) and the core solution of step 2) respectively into an outer needle, having diameter of 0.8-1.2 mm, and inner needle, having diameter of 0.2-1 mm of a coaxial electrostatic spinning needle, and under the voltage of 13-21 kv, receipt distance of 10-30 cm, inner liquid feeding speed of 0.1-0.5 ml / min and outer liquid feeding speed of 0.8-2 ml / min, andconnecting an electrostatic generator to the coaxial electrostatic spinning needle to perform electrostatic spinning so that porous core-shell nano fiber dressing is obtained.

The invention discloses a method for preparing a biomimetic blood vessel stent by composite technology, a blood vessel stent with three-layer structure is prepared by adopting coaxial electrospinningtechnology and coaxial extrusion hollow fiber technology in the field of biological manufacture, and each layer of the blood vessel scaffold can be inoculated with different types of cells between thethree layers of the blood vessel stent, so that the blood vessel stent has a true biomimetic human blood vessel structure; the inner and outer layers can be loaded with drugs, and the middle layer can provide oxygen, nutrients and exclude metabolites for cells. It is helpful to the growth of blood vessel and shortens the period of blood vessel growth, so it has a broad prospect of clinical application.

The invention relates to the field of biomaterials, and particularly relates to a method for preparing PEEK (Polyetheretherketone) biological coatings and a PEEK biomaterial. The PEEK, of which the surface is adhered with polydopamine, is put in a titanium fluoride solution; heat insulation is carried out for 10 hours to 15 hours at 45 DEG C to 55 DEG C and titanium fluoride is deposited; after the surface of the PEEK is adhered with the polydopamine, a titanium fluoride compounded coating is deposited; by using phenolic hydroxyl in the polydopamine as an anchoring point of titanium dioxide, the hybrid compounded coating is prepared through liquid phase deposition. Compared with the prior art, the compounded coating prepared by adopting the method disclosed by the invention has the advantages that the polydopamine and TiO2 are bonded through powerful chemical bonds, so that the bonding effect is very good. The titanium dioxide has an antibacterial effect, so that the finally prepared compounded coating has excellent antibacterial performance. Due to the polydopamine, adhesive growth of the fibroblasts can be promoted, and adhesion, growth and differentiation of the fibroblasts canbe effectively promoted; and finally, the sealing ability of soft tissues is achieved.

The invention provides a double-layer knitting nerve duct having the characteristics that the double-layer knitting nerve duct consists of an outer layer tube wall knitted by polyglycollide-lactide yarns and an inner layer tube wall made from chitin non-woven fabrics, and also provides a method for preparing the double-layer knitting nerve duct having the characteristics that the method comprisesthe following steps of: attaching the chitin non-woven fabrics on a core bar and knitting the polyglycollide-lactide yarns on an outer layer; forming the polyglycollide-lactide yarns for 2 and 5 minutes at a temperature of between 90 and 105 DEG C, cleaning the polyglycollide-lactide yarns with anhydrous alcohol, and forming the polyglycollide-lactide yarns for 1 and 2 minutes at a temperature ofbetween 90 and 100 DEG C; taking down a polyglycollide-lactide knitting layer and a chitin non-woven fabric layer from the core bar at the same time, and freeze-dry the polyglycollide-lactide knitting layer for 2 and 3 hours at a temperature of between 50 below zero and 40 DEG C. The double-layer knitting nerve duct has the advantages of long-distance bridging defect, good quality and fast speed for nerve regeneration and good mechanical property, and contributes to regulation of a pH value in the body.

The invention belongs to the technical field of medical biological materials and discloses a compound biological patch for repairing abdominal wall defects. The compound biological patch is prepared from a biological patch layer and a bletilla striata fiber mesh layer which covers the surface of one side of the biological patch layer, wherein the bletilla striata fiber mesh layer is an electrospunnano-grade bletilla striata fiber mesh. According to the compound biological patch disclosed by the invention, the bletilla striata fiber mesh layer is combined with a traditional biological patch coating film, so that the anti-infection capability and the wound repairing capability of the compound biological patch are effectively improved; meanwhile, after the compound biological patch is implanted into an organism, the mechanical strength change is higher than needed mechanical strength all the time and tissue reconstruction can be accelerated; existing technical bottlenecks of the biological patch are conducive to solving and the treatment effect of repairing the abdominal wall defects is effectively improved.

The polyurethane material possessing cell adhesion growth promoting and thrombolytic functions consists of one base layer of polyurethane material, and one modifying layer with possessing PEG space group connected via covalent bond to the polyurethane material in the base layer and two kinds of bioactive matters, RGD polypeptide and lysine, introduced simultaneously via surface grafting with NHS linked to The PEG space group. It is prepared through: functionating polyurethane material with MDI, surface grafting PEG, introducing active NHS group, grafting RGD polypeptide and lysine, and deprotecting epsilon-NH2 of lysine. The polyurethane material possesses excellent cell adhesion growth promoting function, thrombolytic function, effectively improved blood compatibility and cell compatibility, simple preparation process and low cost, and may be used as biomedicine material.

The invention provides a porous composite bone plate with stiffness and gradient variation and a preparation method thereof. The bone plate is formed by sequentially connecting a bonding layer, a transition layer and a physical layer, wherein the bonding layer, transition layer and physical layer are successively increased in stiffness. The corresponding position of the layers is provided with a positioning hole in a penetrating mode. The bonding layer with smaller stiffness is in contact with the original bone, therefore the stiffness of the contact surface of the bone plate can be reduced and the stress shielding effect is improved; the physical layer with larger stiffness achieves the fixed connection; a porous structure is arranged on the bonding layer and the transition layer respectively, thereby promoting the adhesion growth of cells, forming the more solid biological fixation, and being conducive to the long-term effective service of the bone plate. The bone plate overall structure uses 3D printing to be molded integrally, thus the natural transition and convergence from the porous structure of the bonding layer and transition layer to the physical structure of the physical layer can be achieved; the bone plate possesses obvious heterogeneous mechanical properties. According to the porous composite bone plate with the stiffness and gradient variation and the preparation method thereof, an individualized composite bone plate with stiffness and gradient variation and multi-directional gradient structures can be designed for different parts according to the individual difference, thereby improving the adaptability and biocompatibility of the bone plate.

The invention relates to a degradation controlled tissue engineering comea fibrous scaffold and a preparation method thereof. The basic compositions of the scaffold are gelatin and aliphatic polyesters. The preparation method comprises the following steps: dissolving the gelatin in a mixed solvent to prepare gelatin solution, dissolving the aliphatic polyesters in the gelatin solution, and evenlystirring to prepare mixed polymer spinning solution; and preparing the orderly and disorderly arranged fibrous scaffold through electrostatic spinning, and performing vacuum drying to prepare the degradation controlled tissue engineering comea fibrous scaffold. The gelatin and aliphatic polyesters compounded electrostatic spinning is adopted, crosslinking stabilization is not needed, and the degradation speed of the scaffold and growth speed of corneal cells can be controlled to be synchronous. The product has transmittance of 97 percent, wet tensile strength of 4.91MPa, elongation at ruptureof 956.2 percent, water content of 154.1 percent, and performance parameters similar to human comea, and has good bioactivity and biocompatibility, has controlled degradation and no toxicity, is favorable for adhesion and growth of the cells, and has certain strength and toughness, simple preparation process, low cost and easy wide application.

The invention provides a hydroxyapatite / polyamide composite biological material with a nanofiber cellular structure on the surface. The material is composed of a molding base body and a nanofiber layer which covers the surface of the molding base body and is combined with the molding base body integrally; nanofibers in the nanofiber layer are staggered to form the cellular structure, and the molding base body and the nanofiber layer are the hydroxyapatite / polyamide composite biological material. A preparing method includes the following steps: the hydroxyapatite / polyamide composite biological material and calcium chloride are dissolved into absolute ethyl alcohol to form a spinning solution; the molding base body is arranged on a receiving screen, and the spinning solution is coated on the molding base body in a spinning mode with an electrostatic spinning method, and the hydroxyapatite / polyamide composite biological material is obtained. By means of the hydroxyapatite / polyamide composite biological material, adherence growth of cells and tissues are facilitated, vascularization is easily achieved after the hydroxyapatite / polyamide composite biological material is implanted, and the combination performance between the hydroxyapatite / polyamide composite biological material and the bone tissues is good.

The invention discloses a bone repair material with a multi-dimensional channel structure. The bone repair material has a layered matrix structure; a plurality of bosses I are formed in one surface of the matrix structure, and a plurality of bosses II are formed in another surface of the matrix structure; the bosses I and the bosses II are arrayed alternatively; grooves are formed between adjacent bosses I and adjacent bosses II; and a plurality of through holes are formed in a way of being perpendicular to the surfaces of the bosses I. The bone repair material provided by the invention adopts a three-dimensional network channel formed through the through holes in the matrix structure and the grooves, and the three-dimensional network channel is filled with collagenous fiber, which is favorable for cell attachment and growth as well as nerve ingrowth, and also is convenient for nutrient substance transfer and cell metabolite discharge, and also can enable cells to be evenly distributed in the interior of the material, and is favorable for even and uniform organizational formation in the interior of a support; the structural material can form firm biological gomphosis with regenerated new bone, and good structure stability can be maintained during a degradation process; the mechanical strength is high, and mechanical transmission between a bone defect part and normal bone tissue can be well achieved.

The invention aims to provide an artificial soft tissue braided fabric which has excellent mechanical properties, such as dynamic properties and facilitates cell growth and a preparation method for the braided fabric. The artificial soft tissue braided fabric disclosed by the invention comprises a first surface layer, a first middle layer, an inner layer, a second middle layer and a second surface layer in turn; the first surface layer and the second surface layer are locally or wholly prepared from silks; the first middle layer and the second middle layer are locally or wholly prepared from PET; and the inner layer is locally or wholly prepared from PEHM.

The invention relates to a method for preparing polypeptide copolymer porous nanofiber by using electrostatic spinning. The method comprises the following steps: firstly, carrying out hydrophilic modification on a hydrophobic polypeptide homopolymer to prepare a poly(gamma-benzyl L-glutamate-hydroxyethyl glutamine) or poly(gamma-benzyl L-glutamine)-g-polyethylene glycol grafted copolymer; secondly, preparing a compound solvent by using solvents with different volatilization properties, and then preparing into a spinning solution; thirdly, spinning by using a high voltage electrostatic spinning method; and finally, placing spun yarns into a vacuum drying chamber and drying for more than 3 hours at room temperature to obtain the porous nanofiber with high specific surface area. A cytotoxicity test indicates that the prepared porous nanofiber with high specific surface area is free of cytotoxicity and good in biocompatibility, can be used as a porous scaffold for cell growth, has the function of promoting cell migration and amplification and has a relatively good application value in the aspect of preparation of scaffolds for tissue engineering; besides, the polypeptide copolymer porous nanofiber has an application prospect in the fields of medical dressing, medicine released control, artificial organs, sewage treatment and the like.

The invention relates to a medical degradable bioglass / phytic acid composite coating on the surface of magnesium alloy and a preparation method thereof. The medical degradable bioglass / phytic acid composite coating is characterized in that an inner layer of the composite coating is a bioglass 45S5 coating with the mesoporous diameter in the range from 2 to 5nm, and an outer layer of the composite coating is a phytic acid coating with uniform and compact structure. The thickness of the composite coating is in the range from 0.6 to 1.2 micrometers, the composite coating has very good combination property with a magnesium alloy matrix, and the combination strength reaches 6-15MPa. The average degradation rate of the sample soaked in SBF (Simulated Body Fluid) is 6.76*10<-7>g / (mm<2>h) within 240h. The phytic acid coating is prepared outside the mesoporous bioglass, so that phytic acid magnesium is generated through the reaction between phytic acid and the magnesium alloy so as to block the pores of the mesoporous glass, thus the degradation period of the magnesium alloy is effectively adjusted and controlled, the biocompatibility of the material can be improved, and the generation of apatite is accelerated and induced.

The invention provides biodegradable polymer microparticles and a preparation method thereof. The polymer microparticles is a copolymer of lactic acid and / or glycolic acid repeating unit, and the particle sizes of the polymer microparticles are 10 micrometers to 150 micrometers. The PLLA microparticles disclosed by the invention has an irregular, unsmooth or rough microscopic shape, so that the contact area of the PLLA particles and cells is increased, the adhesion ability and the retention time of the cells on a porous structure or a scaffold structure are improved and prolonged, and the cellaffinity performance of the PLLA particles is obviously improved; the biodegradable polymer microparticles are beneficial for stimulating collagenocyte to feel physical and mechanical micro-environment stimulation and make a response, so that growth of collagen of a human body is further stimulated, and the effects of removing wrinkles, maintaining beauty and delaying aging are natural and lasting.

Belonging to the field of biological tissue engineering and medical materials, the invention relates to a decellularized cerebellar extracellular matrix regeneration biological scaffold containing multiple neurotrophic factors and a preparation method thereof. As a cerebellum regeneration scaffold material, the natural cerebellar decellularized scaffold adopted by the invention is a three-dimensional network structure, and the spatial configuration conforms to the cerebellar physiological structure spatial configuration. The regeneration biological scaffold and its biomechanical properties are similar to normal cerebellus, and can provide physiologically similar channels for reconstruction of nerve pathways so as to provide correct guidance for growth. The scaffold surface has cell-membrane receptor recognition sites and partially active factors, thus being conducive to adhesion growth of cells and functioning of physiological functions. Engineered seed cells can secrete a variety of neurotrophic / growth factors, also can promote the formation of new extracellular matrixes, and provides regeneration guidance signals. With good biocompatibility, the regeneration biological scaffold can be used for preparation of neural repair preparations for treatment of nerve injuries.

The invention discloses a 3D printing scaffold material for icariin (ICA)-loaded PLGA microspheres, wherein the 3D printing scaffold material can be used for skull defect repairing. An ICA functionaldrug is adopted, endogenous osteoblasts are collected to a skull defect area to prompt osteoblast differentiation of the endogenous osteoblasts, meanwhile based on 3D printing design microspheres anda nano-hydroxyapatite micro-nano structure composite scaffold, an individualized bionic degradable polymer scaffold is prepared and can be absorbed and degraded in human bodies to avoid side effects of second operation and implantation materials, the effects of bone conduction and bone induction are achieved, bone tissue regeneration is prompted, and bone healing is prompted.

The invention discloses a preparation method of a polyhydroxyalkanoate / polypyrrole composite electrospun membrane and an electrospinning membrane; the preparation method comprises the steps of preparing a polyhydroxyalkanoate fiber electrospun membrane through an electrospinning process, and then preparing the polyhydroxyalkanoate / polypyrrole composite electrospun membrane through a surface modification process. The preparation method is simple and rapid, raw materials are easy to obtain, and the prepared polyhydroxyalkanoate / polypyrrole composite electrospun membrane has good hydrophilicity and cell compatibility, can provide durable and stable skeleton structure supporting after being implanted into tissues, can regulate cell attachment and cell migration and induce tissue differentiation under electrical stimulation, and has wide application prospects in the bioengineering fields such as oriented repair of tissue.

The invention relates to new use of carbonized cuttlebones, in particular to application of carbonized cuttlebones in preparation of medical materials for treating diseases of bone defect, bone tumor and cyst. The medical materials can be bone tissue engineering scaffold materials or bone grafting substitution materials. The invention has the advantages that: the carbonized cuttlebones have excellent microporous structure and biocompatibility, is beneficial to osteoblast adherence growth and promotes bone differentiation to a certain extent. The microwave carbonized cuttlebones can be used as the bone tissue engineering scaffold materials or bone grafting substitution materials, therefore, the invention is hopeful to be used for clinical treatment and repairing of dead space after the cutting of bone defect, bone tumor and cyst, promotes combination of vertebral column and joints, and can be used as a cell carrier to construct tissue-engineered bones in vitro in a compounding way.

The invention discloses a stent for directional induction of neural stem cell differentiation and a preparation method thereof, and belongs to the field of polymer materials and biomedical engineering. The adhesion growth of cells is promoted by improving the hydrophilicity of a matrix material of the stent; a microgroove structure is constructed on the inner and outer surfaces of the stent material, and the function of directional induction of the differentiation is achieved; at the same time, the stent can further release biological active factors in the controlled mode and promote the adhesion and the growth of the stem cells after the directional differentiation. The stent has a good application prospect in neural stem cell transplantation.