The invention discloses a magnetic 3D gel guide 
pipe for repairing nerve defects and a preparation method and application thereof. The magnetic 3D gel guide 
pipe is prepared from the following components in parts by weight: 97-99 parts of an 
alginic acid / 
chitosan mixture, 0.8-1.5 parts of Fe3O4 and 0.8-1.5 parts of GO, the magnetic 3D gel guide 
pipe is of a spiral structure, the screw 
pitch is 30[mu]m-60[mu]m, the inner 
diameter of the guide pipe is 1mm-8mm, and the outer 
diameter of the guide pipe is 2mm-10mm. The preparation method of the magnetic 3D gel guide pipe comprises the following steps that a 
spinning solution is prepared, specifically, the 
alginic acid / 
chitosan mixed solution, a Fe3O4 dispersion solution and a GO dispersion solution are mixed, and normal-temperature ultrasonictreatment is carried out to obtain a 
spinning solution; and the magnetic 3D gel guide pipe is prepared, the 
spinning solution is pumped into a micro-fluidic 
chip from a main 
liquid phase inflow channel, a 
calcium chloride solution and absolute ethyl 
alcohol are pumped into the micro-fluidic 
chip from different auxiliary 
liquid phase channels, the pumping speeds of the spinning solution, the 
calcium chloride and the absolute ethyl 
alcohol is adjusted, and the magnetic 3D gel guide pipe is prepared. According to the magnetic 3D gel guide pipe for repairing the nerve defects and the preparation method and application thereof, a microfluidic technology is adopted, and the prepared guide pipe can safely and effectively promote rapid repair of 
peripheral nerve defects.