75results about How to "Realize high-throughput automated detection" patented technology

The invention discloses a multi-sample multi-site typing method against single nucleotide polymorphisms (SNP) in a genome by using magnetic nano particles as carriers and combining with the technology of universal label probe. The invention is characterized in that: a labeled PCR product is directly fixed on the magnetic nano particles through covalent unions between labels, or a labeled primer is fixed on the magnetic nano particles to directly execute solid-phase PCR amplification, thereby forming magnetic nano particles-DNA complex (MNP-ssDNA). Then, the MNPs-ssDNA is hybridized with a detecting probe of corresponding site and a labeled universal label probe, thereby achieving multi-sample multi-site high flux SNP detection. The advantages of the invention are that: only a pair of labeled probes are required by using the universal label technology to achieve the multi-site typing, and the typing cost is greatly reduced; meanwhile, the invention overcomes the defects of high cost, time consuming, taking trouble, etc. caused by purifying and concentrating a target sequence waiting for detecting in the prior art by using the advantages that the magnetic nano particles are easily separated, etc. Therefore, the method has the advantages of typing mass samples simply, efficiently and accurately.

The invention provides a medication method and a kit of an antihypertensive drug irbesartan. The method comprises the following steps: respectively designing multiple amplification primers and extension primers according to a plurality of to-be-detected target SNP loci; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in a reaction system, using aplurality of primers for simultaneously and respectively carrying out amplification and single base extension reaction on a plurality of target SNP sites; carrying out flight time mass spectrometry analysis on a product obtained after the single base extension reaction, identifying genotypes of SNP related to different drug metabolism according to products of extension primers with different molecular weights represented by mass spectrometry peaks, and guiding medication of the antihypertensive drug irbesartan. The invention also provides the detection kit using the method. The SNP locus related to metabolism of the antihypertensive drug irbesartan can be detected, and the SNP locus has the advantages of being low in cost, free of probe synthesis, short in consumed time, simple and convenient in result analysis and extremely wide in application field.

The invention provides a method and kit for guiding medication of nitrendipine. The method comprises steps as follows: multiplex amplification primers and an extension primer are designed respectivelyaccording to two to-be-detected target SNP sites; a multiplex amplification primer reaction system and an extension primer reaction system are prepared; in the reaction systems, an amplification reaction and a single base extension reaction are performed on the two target SNP sites respectively by using multiple sets of primers; products obtained by the single base extension reaction are subjected to time-of-flight mass spectrometry analysis, according to extension primer products with different molecular weights and represented by mass spectrum peaks, genotypes of different SNPs related to drug metabolism are identified, and medication of the nitrendipine drug for reducing high blood pressure is guided. Besides, the invention also provides a detection kit using the method. The kit can detect two SNP sites related to metabolism of the nitrendipine drug for reducing high blood pressure simultaneously and has the advantages that the kit is low in cost, doesn't need synthesis probe, takes short time and is simple and convenient in result analysis and very broad in application field.

The invention discloses a method for carrying out mass-spectrography differentiation on nitrendipine individualized medication through a detection product. The method comprises the following steps of:according to two target SNP (single nucleotide polymorphism) sites to be detected, independently designing multiplex amplification primers and an extension primer; preparing a multiplex amplificationprimer reaction system and an extension primer reaction system; in the reaction systems, adopting multiple sets of primers to simultaneously independently carry out an amplification reaction and a single base extension reaction on the two target SNP sites; carrying out time-of-flight mass spectrometry analysis on products obtained by the single base extension reaction; according to extension primer products with different molecular weights and represented by mass spectrometry peaks, identifying the genotypes of different SNPs related to medicine metabolism; and guiding the individualized medication of a hypertension-reducing medicine, i.e., nitrendipine. The method can simultaneously detect the two SNP sites related to nitrendipin metabolism, has the advantages of low cost, short time consumption and an extremely wide application field and does not need to carry out probe synthesis, and result analysis is simple and convenient.

The invention provides a detection product for judging individualized medication type of nitrendipine. A preparation method comprises steps as follows: multiplex amplification primers and an extensionprimer are designed separately according to two to-be-detected target SNP sites; a multiplex amplification primer reaction system and an extension primer reaction system are prepared; in the reactionsystems, an amplification reaction and a single base extension reaction are performed on the two target SNP sites respectively by using multiple sets of primers; products obtained by the single baseextension reaction are subjected to time-of-flight mass spectrometry, according to extension primer products with different molecular weights and represented by mass spectrum peaks, genotypes of different SNPs related to drug metabolism are identified, and individualized medication of the nitrendipine drug for reducing high blood pressure is guided. The detection product can detect two SNP sites related to metabolism of the nitrendipine simultaneously and has the advantages that the detection product is low in cost, doesn't need probe synthesis, takes short time and is simple and convenient inresult analysis and very broad in application field.

The invention provides a medicine using method and a kit of hypertension reduction medicine telmisartan. The method comprises the following steps: separately designing multiple amplification primers and extension primers according to a plurality of target SNP locus to be detected; preparing multiple amplification primer reaction systems and extension primer reaction systems; simultaneously performing amplification and single base extension reaction on the plurality of target SNP locus by using multiple sets of primers in the reaction systems; performing flight time mass spectrometry analysis on the product subjected to the single base extension reaction, verifying gene types of metabolism-related SNP of different medicines, and guiding using of the hypertension reduction medicine telmisartan. Meanwhile, the invention provides the detection kit using the method. According to the medicine using method and the kit disclosed by the invention, SNP locus related with the metabolism of the hypertension reduction medicine telmisartan can be detected, and the kit has the advantages of being low in cost, having no need of synthetic probe, being short in time consumption, being simple and convenient in result analysis, and being extremely wide in application field, and can be used for aided diagnosis and treatment of hypertension patients who clinically need oral administration of the telmisartan.

The invention provides a detection product for mass-spectrometry identification of personalized medication of nitrendipine through a primer composition. A preparation method of the detection product includes the steps of: designing multiple-amplification primers and extension primers separately according to two to-be-detected target SNP sites, preparing a multiple amplification primer reaction system and an extension primer reaction system, performing amplification and single-base extension reactions on the two target SNP sites simultaneously through multiple sets of primers in the reaction systems, performing time-of-flight mass spectrometry analysis on products obtained after the single-base extension reactions, identifying the genotypes of different SNPs related to metabolism of the drug according to extension primer products which are represented by mass spectrum peaks and have different molecular weights, and guiding personalized medication of the antihypertensive drug nitrendipine. The two SNP sites related to metabolism of the nitrendipine drug can be detected simultaneously, and the product has the advantages of low cost, no needs for synthetic probes, short time consumption, simple and convenient result analysis and extremely wide application fields.

The invention provides a method and a kit for guiding medication of nitrendipine and atenolol. The method comprises the following steps: respectively designing multiple amplification primers and extension primers according to 10 to-be-detected target SNP loci; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, simultaneouslyand respectively carrying out amplification and single-base extension reaction on the 10 target SNP loci by using a plurality of sets of primers; and carrying out time-of-flight mass spectrometry analysis on a product after the single base extension reaction, identifying genotypes of SNP related to different drug metabolism according to products of extension primers with different molecular weights represented by a mass spectrum peak, and guiding the medication of antihypertensive drug nitrendipine and atenolol. Meanwhile, the invention provides the detection kit using the method. The methodcan simultaneously detect the 10 SNP loci related to metabolism of the antihypertensive drug nitrendipine and atenolol, and has the advantages of low cost, no need of probe synthesis, short time consumption, simple and convenient result analysis and extremely wide application field.

The invention provides a primer composition for identifying personalized medication of nitrendipine. The preparation method of the primer composition includes the steps: designing multiple-amplification primers and extension primers separately according to two to-be-detected target SNP sites, preparing a multiple amplification primer reaction system and an extension primer reaction system, performing amplification and single-base extension reactions on the two target SNP sites simultaneously through multiple sets of primers in the reaction systems, performing time-of-flight mass spectrometry analysis of products obtained after the single-base extension reactions, identifying the genotypes of different SNPs related to metabolism of the drug according to extension-primer products which are represented by mass spectra and have different molecular weights, and guiding personalized medication of the antihypertensive drug nitrendipine. The two SNP sites related to metabolism of the nitrendipine drug can be detected simultaneously, and the product has the advantages of low cost, no needs for synthetic probes, short time consumption, simple and convenient result analysis and extremely wideapplication fields.

The invention provides a method utilizing extension primers with different molecular weights at different SNP (single nucleotide polymorphism) sites to detect multiple sites related to metabolism of an antihypertensive drug lacidipine by MALDI-TOF-MS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) and guide medication of lacidipine finally as well as a kit. The method comprises steps as follows: designing multiple amplification primers and extension primers respectively according to six target SNP sites to be detected; preparing a multi-amplification primer reaction system and an extension reaction system; performing amplification and single-base extension reactions on the six target SNP sites by multiple primers simultaneously respectively; performing time-of-flight mass spectrometry on products obtained after the single-base extension reaction, identifying genetypes of SNPs related to different drug metabolisms according to products, represented by massspectrum peaks, of extension primers with different molecular weights, and guiding the medication of the antihypertensive drug lacidipine. Meanwhile, the invention provides a detection kit utilizingthe method. The SNP sites related to metabolism of six antihypertensive drug lacidipine can be detected simultaneously, and the method has the advantages of low cost, no need of probe synthesis, low time consumption, simple and convenient result analysis and quite wide application field.

The invention provides a mass spectrography method for differentiation of individualized medication of lacidipine through a primer composition. According to different single nucleotide polymorphism (SNP) sites having extension primers with different molecular weights, through matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), a plurality of sites pertinentto metabolism of a hypertension reducing medicine namely lacidipine can be detected at the same time, and finally the mass spectrography method for differentiation of individualized medication of lacidipine through the primer composition is obtained. The method comprises the steps of: according to 6 target SNP sites to be detected, separately designing multiplex amplification primers and an extension primer; compounding a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, performing an amplification reaction and a single-base extension reaction on the 6 target SNP sites at the same time with multiple sets of primers; and performing time-of-flight mass spectrometric analysis on products after the single-base extension reaction,identifying the genotypes of different drug metabolism relevant SNPs according to extension primer products with different molecular weights and represented by mass spectrum peaks, and guiding the medication of the hypertension reducing medicine namely the lacidipine. The method disclosed by the invention can detect 6 metabolism pertinent SNP sites of the hypertension reducing medicine namely thelacidipine at the same time, and has the advantages of being low in cost, free from probe synthesis, short in time consumption, simple and convenient in result analysis, and extremely broad in application field.

The invention provides a detection product for discriminating the individualized medication type of nitrendipine and atenolol. The method comprises the following steps: respectively designing multipleamplification primers and extension primers according to 10 to-be-detected target SNP loci; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in thereaction systems, simultaneously and respectively carrying out amplification and single-base extension reaction on the 10 target SNP loci by using a plurality of sets of primers; and carrying out time-of-flight mass spectrometry analysis on a product after the single base extension reaction, identifying genotypes of SNP related to different drug metabolism according to products of extension primers with different molecular weights represented by a mass spectrum peak, and guiding the medication of antihypertensive drug nitrendipine and atenolol. The method can simultaneously detect the 10 SNP loci related to metabolism of the antihypertensive drug nitrendipine and atenolol, and has the advantages of low cost, no need of probe synthesis, short time consumption, simple and convenient result analysis and extremely wide application field.

The invention relates to the field of tumor diagnosis, and discloses establishment of a composition for early diagnosis of ovarian cancer, ovarian cancer can be predicted, diagnosed and / or prognostically evaluated by measuring cfDNA content, cfDNA TP53 mutation abundance and CA125 protein expression level, the sensitivity, specificity and accuracy of the composition reach 91.11%, 94.34% and 93.38%, and the detection rate of early ovarian cancer reaches 78.9%. By adopting the marker combination and the model scoring method disclosed by the invention, 71.05% of patients can be detected in CA125 negative ovarian cancer patients, 74% of subjects can be correctly judged in CA125 positive non-tumor people, and the problems of CA125 leak detection and error detection can be well solved. According to the invention, an effective early ovarian cancer diagnosis or screening marker can be provided clinically, and the prediction accuracy and the detection rate of the early ovarian cancer can be improved.

The invention discloses a high-throughput virus detection device and application thereof in detection of novel coronavirus. The detection device comprises a virus sample adding unit; a test paper batch feeding unit which is provided with a test paper storage tank, wherein the bottom of the test paper storage tank is provided with a stored test paper outlet, and the test paper storage tank is used for stacking test paper; a test paper collecting and buffering unit which is provided with a test paper collecting groove, wherein the bottom of the test paper collecting groove is provided with a collected test paper inlet, and the test paper collecting groove is used for stacking test paper after virus samples are added; a test paper transfer unit which is provided with a test paper clamp and an operation power part, wherein the motion path of the test paper clamp for transporting the test paper is set to pass through the test paper storage tank, the sample adding table and the test paper collecting tank in sequence; a data acquisition unit; and a result detection motion unit which is used for conveying the detection test paper in the test paper collecting tank to the data acquisition unit. According to the invention, automatic detection of high-throughput novel coronavirus can be realized, the detection efficiency is improved, and the risk of cross infection is avoided.

The invention provides a method and kit for guiding administration of verapamil by simultaneously detecting multiple loci related to the metabolism of the antihypertensive drug verapamil through matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) because different single nucleotide polymorphism (SNP) loci have extension primers with different molecular weights. The method includes the following steps: designing multiple amplification primers and extension primers according to eight target SNP loci to be detected; preparing a multiple amplification primerreaction system and an extension primer reaction system; in the reaction systems, simultaneously performing amplification and single-base extension reactions on the eight target SNP loci by using multiple sets of primers; and performing time-of-flight mass spectrometry analysis on the products after the single-base extension reactions, and identifying the genotypes of different drug metabolism-related SNP loci according to the products of different-molecular-weight extension primers represented by mass spectrum peaks to guide the administration of the antihypertensive drug verapamil. At the same time, the invention provides the detection kit using the method. The method can simultaneously detect the eight SNP loci related to the metabolism of the antihypertensive drug verapamil, and has the advantages of low costs, no need for synthesis of probes, short time consumption, simple and convenient result analysis, and extremely wide application fields.

The invention provides a method for identifying individualized administration of verapamil by performing mass spectrometry through detection products. The method comprises the following steps: designing multiple amplification primers and extension primers according to eight target SNP loci to be detected; preparing a multiple amplification primer reaction system and an extension primer reaction system; in the reaction systems, simultaneously performing amplification and single-base extension reactions on the eight target SNP loci by using multiple sets of primers; and performing time-of-flightmass spectrometry analysis on the products after the single-base extension reactions, and identifying the genotypes of different drug metabolism-related SNP loci according to the products of different-molecular-weight extension primers represented by mass spectrum peaks to guide the administration of the antihypertensive drug verapamil. The method can simultaneously detect the eight SNP loci related to the metabolism of the antihypertensive drug verapamil, and has the advantages of low costs, no need for synthesis of probes, short time consumption, simple and convenient result analysis, and extremely wide application fields.

The invention provides a detection product for distinguishing individualized medication types of trandolapril as an antihypertensive drug. A preparation method of the detection product comprises the following steps: designing multiplex amplification primers and extension primers respectively according to a plurality of to-be-detected target SNP (single nucleotide polymorphism) loci; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, performing amplification and single base extension reactions on the target SNP loci simultaneously and respectively by multiple sets of primers; performing time-of-flight mass spectrometry on a product obtained after the single base extension reaction, identifying genotypes of SNP relatedto metabolism of different drugs, and guiding individualized medication of the trandolapril as the antihypertensive drug. The detection product can detect five SNP loci related to trandolapril drug metabolism simultaneously, has the advantages of low cost, no need of synthetic probes, short consumed time, simple and convenient result analysis and extremely wide application field, and can be usedfor auxiliary diagnosis and treatment of hypertensive patients requiring to orally take the trandolapril clinically.

The invention provides a mass spectrometry method for identifying personalized medication of trandolapril through product detection. The method includes the steps: designing multiple-amplification primers and extension primers separately according to multiple to-be-detected target SNP sites, preparing a multiple amplification primer reaction system and an extension primer reaction system, performing amplification and single-base extension reactions at the target SNP sites simultaneously through multiple sets of primers in the reaction systems, performing time-of-flight mass spectrometry analysis of products obtained after the single-base extension reactions so as to identify the genotypes of SNP related to metabolism of different drugs, and guiding personalized medication of the antihypertensive drug trandolapril. Five SNP sites related to metabolism of the trandolapril drugs can be detected simultaneously, and the method has the advantages of low cost, no needs for synthetic probes, short time consumption, simple and convenient result analysis and extremely wide application fields, and can be applied to auxiliary diagnosis and treatment of hypertensive patients who need to take trandolapril clinically.

The invention provides a medication method and reagent kit of a hypertension reducing medicine namely trandolapril. The medication method comprises the steps of according to multiple objective SNP sites to be detected, respectively designing a multiplex amplification primer and an extension primer; compounding a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, performing amplification and a single-base extension reaction on the objective SNP sites at the same time with multiple sets of primers; and performing flight time mass spectrometric analysis on products after being subjected to the single-base extension reaction, identifying the genotypes of different drug metabolism relevant SNPs according to products of different molecular weight extension primers represented by mass spectrum peaks, and guiding the medication of the hypertension reducing medicine namely the trandolapril. Besides, the invention provides a detectionreagent kit using the method. According to the medication method disclosed by the invention, 5 trandolapril drug metabolism pertinent SNP sites can be detected at the same time, and the medication method has the advantages of being low in cost, free from synthetized probes, short in time consumption, simple and convenient in result analysis, and extremely broad in application field.

The invention provides a method for determination of individualized medication of lacidipine through mass spectrography using detection products. Different single nucleotide polymorphism (SNP) sites have extension primers with different molecular weights, so that through matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), a plurality of sites pertinent to metabolism of a hypertension reducing medicine namely lacidipine can be detected at the same time, and finally, the method for determination of individualized medication of lacidipine through mass spectrography using the detection products is obtained. The method comprises the steps of: according to 6 target SNP sites to be detected, separately designing multiplex amplification primers and an extension primer; compounding a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, performing amplification and a single-base extension reaction on the 6 target SNP sites at the same time with multiple sets of primers; and performing time-of-flight mass spectrometric analysis on products after the single-base extension reaction, identifyingthe genotypes of different SNPs relevant to drug metabolism according to extension primer products with different molecular weights represented by mass spectrum peaks, and guiding the medication of the hypertension reducing medicine namely the lacidipine. The method disclosed by the invention can detect 6 SNP sites pertinent to metabolism of the hypertension reducing medicine namely the lacidipineat the same time, and has the advantages of being low in cost, free from probe synthesis, short in time consumption, simple and convenient in result analysis, and extremely broad in application field.

The invention provides a method for carrying out mass-spectrography distinguishing on trandolapril individualized medicine application through a primer composition. The method comprises the followingsteps of: according to a plurality of objective SNP sites to be detected, independently designing a multiplex amplification primer and an extension primer; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, adopting multiple sets of primers to simultaneously independently carry out amplification and single-base extension reaction on the plurality of objective SNP (single nucleotide polymorphism) sites; and carrying out flight time mass spectrometry on a product subjected to the single-base extension reaction, identifyingthe genotypes of different medicine metabolism related SNPs, and guiding the individualized medicine application of a hypertension lowering medicine, i.e., the trandolapril. The method disclosed by the invention can simultaneously detect five trandolapril medicine metabolism related SNP sites, has the advantages of low cost, short time consumption, simple and convenient result analysis and wide application ranges, does not need to carry out probe synthesis, and can be used for the auxiliary diagnosis and treatment of a hypertension patient who clinically needs to take the trandolapril.

The invention provides a primer composition for distinguishing an individualized medicinal form of nitrendipine and atenolol. A method for distinguishing the individualized medicinal form of the nitrendipine and atenolol includes the following steps: designing multiple amplification primers and extension primers respectively according to 10 target SNP loci to be tested; preparing a multiple amplification primer reaction system and an extension primer reaction system; in the reaction systems, simultaneously performing amplification and single base extension reactions on the 10 target SNP loci by using multiple sets of primers; and performing time-of-flight mass spectrometry analysis on the product after the single base extension reaction, and according to the products of different molecular-weight extension primers represented by mass spectrum peaks, identifying the genotypes of different drug metabolism-related SNPs to guide the administration of the antihypertensive drug nitrendipineand atenolol. The composition can simultaneously detect 10 SNP loci related to the metabolism of the antihypertensive drug nitrendipine and atenolol, and has the advantages of low costs, no need for synthesis of probes, short time consumption, simple and convenient analysis of results, and extremely wide application fields.

The invention provides a method for distinguishing an individualized medicinal form of nitrendipine and atenolol through mass spectrometry by a primer composition. The method includes the following steps: designing multiple amplification primers and extension primers respectively according to 10 target SNP loci to be tested; preparing a multiple amplification primer reaction system and an extension primer reaction system; in the reaction systems, simultaneously performing amplification and single base extension reactions on the 10 target SNP loci by using multiple sets of primers; and performing time-of-flight mass spectrometry analysis on the product after the single base extension reaction, and according to the products of different molecular-weight extension primers represented by massspectrum peaks, identifying the genotypes of different drug metabolism-related SNPs to guide the administration of the antihypertensive drug nitrendipine and atenolol. The method can simultaneously detect 10 SNP loci related to the metabolism of the antihypertensive drug nitrendipine and atenolol, and has the advantages of low costs, no need for synthesis of probes, short time consumption, simpleand convenient analysis of results, and extremely wide application fields.

The invention provides a primer composition for distinguishing an individualized medication type of an antihypertensive drug trandolapril. A preparation method of the primer composition comprises thefollowing steps: multiple amplification primers and extension primers for multiple to-be-detected target SNP loci respectively; a multiple amplification primer reaction system and an extension primerreaction system are prepared; in the reaction systems, amplification and single-base extension reaction is conducted simultaneously and respectively for the multiple target SNP loci by using multipleprimers; and products obtained after the single-base extension reaction are subjected to time-of-flight mass spectrometry (TOF MS) analysis to identify genotypes of different SNPs related to drug metabolism and guide individualized medication of the antihypertensive drug trandolapril. The primer composition can detect five SNP loci related to trandolapril drug metabolism simultaneously, has the advantages of low cost, no synthesis probe, short time, simple and convenient result analysis and very wide application fields, and can be applied to auxiliary diagnosis and therapy of hypertension patients needing to take trandolapril clinically.

The invention provides a method for discriminating the individualized medication type of nitrendipine and atenolol through mass spectrometry by detecting a product. The method comprises the followingsteps: respectively designing multiple amplification primers and extension primers according to 10 to-be-detected target SNP loci; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, simultaneously and respectively carrying out amplification and single-base extension reaction on the 10 target SNP loci by using a plurality ofsets of primers; and carrying out time-of-flight mass spectrometry analysis on a product after the single base extension reaction, identifying genotypes of SNP related to different drug metabolism according to products of extension primers with different molecular weights represented by a mass spectrum peak, and guiding the medication of antihypertensive drug nitrendipine and atenolol. The methodcan simultaneously detect the 10 SNP loci related to metabolism of the antihypertensive drug nitrendipine and atenolol, and has the advantages of low cost, no need of probe synthesis, short time consumption, simple and convenient result analysis and extremely wide application field.

The invention provides a method and a medication method of antihypertensive drug ramipril and a gene detection test kit, wherein extension primers with different molecular weights at different singlenucleotide polymorphism (SNP) sites are utilized, therefore a plurality of sites can be simultaneously detected by a matrix-assisted laser desorption-ionization time of flight mass spectrometry(MALDI-TOF MS). The kit can simultaneously detect two ramipril drug metabolism related SNP sites, has the advantages of low cost, no need of probe synthesis, short time consumption, simple and convenient result analysis and extremely wide application field, and can effectively reduce occurrence of adverse events related to ramipril use amount and prevention of thrombotic diseases. The ramipril injectioncan be used for auxiliary diagnosis and treatment of hypertension patients who need to take ramipril clinically.

The invention provides a primer combination for differentiating verapamil individualized medication types. An extension primer which has different molecular weights at different single nucleotide polymorphism (SNP) sites is utilized, so that through matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), a plurality of sites pertinent to metabolism of a hypertension reducing medicine namely verapamil can be detected at the same time, and finally the primer combination for differentiating verapamil individualized medication types is obtained. A preparation method of the primer combination comprises the steps of according to 8 objective SNP sites to be detected, respectively designing a multiplex amplification primer and an extension primer; compounding amultiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, performing amplification and a single-base extension reaction on the 8 objective SNP sites at the same time with multiple sets of primers; and performing time-of-flight mass spectrometry analysis on products after the single-base extension reaction, identifying the genotypes of different drug metabolism pertinent SNPs according to products of different molecular weight extension primers represented by mass spectrum peaks, and guiding the medication of the hypertension reducing medicine namely the verapamil. The primer combination disclosed by the invention can detect 8 metabolism pertinent SNP sites of the hypertension reducing medicine namely the verapamil at the same time, and has the advantages of being low in cost, free from synthetized probes, short in time consumption, simple and convenient in result analysis, and extremely broad in application field.

The invention provides a detection product for judging individualized medication of benazepril. A preparation method comprises the following steps: respectively designing multiple amplification primers and extension primers according to a plurality of to-be-detected target SNP sites; preparing a multiplex amplification primer reaction system and an extension primer reaction system; in a reaction system, simultaneously and respectively carrying out amplification and single base extension reaction on the plurality of target SNP sites by using a plurality of primers; carrying out time-of-flight mass spectrometry analysis on a product after the single base extension reaction, identifying genotypes of SNP related to different drug metabolism according to products of extension primers with different molecular weights represented by mass spectrum peaks, and guiding individualized medication of antihypertensive drug benazepril. The detection product can simultaneously detect 9 SNP sites related to benazepril drug metabolism, and has the advantages of low cost, no need of probe synthesis, short time consumption, simple and convenient result analysis and extremely wide application field.

The invention provides a primer composition for distinguishing an individualized medication type of an antihypertensive drug valsartan. A method for distinguishing individualized medication type of valsartan comprises the following steps: respectively designing multiple amplification primers and extension primers according to multiple target SNP sites to be detected; preparing a multiple amplification primer reaction system and an extension primer reaction system; in a reaction system, using multiple sets of primers to simultaneously perform amplification and single base extension reactions onthe multiple target SNP sites respectively; performing time-of-flight mass spectrometry analysis on the products after the single base extension reaction, identifying genotypes of different drug metabolism related SNPs according to the products of different molecular weight extension primers represented by mass spectrum peaks, and guiding the individualized medication of the antihypertensive drugvalsartan. The primer composition can be used for simultaneously detecting the SNP sites related to metabolism of the antihypertensive drug valsartan, and has advantages of low cost, no need of probesynthesis, short time consumption, simple and convenient result analysis and extremely wide application field.

The invention provides a detection product for differentiating enalapril individualized medication types. An extension primer which has different molecular weights at different single nucleotide polymorphism (SNP) sites is utilized, so that through matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MassArray matrix-assisted laser desorption ionisation-time-of-flight massspectrometry, MALDI-TOF MS), a plurality of sites can be detected at the same time, and finally the detection product for differentiating enalapril individualized medication types is obtained. A preparation method of the detection product comprises the steps of according to a plurality of objective SNP sites to be detected, respectively designing a multiplex amplification primer and an extensionprimer; compounding a multiplex amplification primer reaction system and an extension primer reaction system; in the reaction systems, performing amplification and a single-base extension reaction onthe objective SNP sites at the same time with multiple sets of primers; and performing time-of-flight mass spectrometric analysis on products after the single-base extension reaction, identifying thegenotypes of different drug metabolism relevant SNPs according to products of different molecular weight extension primers represented by mass spectrum peaks, and guiding the medication of the hypertension reducing medicine namely the enalapril. The detection product disclosed by the invention can detect 6 enalapril drug metabolism pertinent SNP sites at the same time, and has the advantages of being low in cost, free from synthetized probes, short in time consumption, simple and convenient in result analysis, and extremely broad in application field.