The invention discloses a method for detecting 
drug residues in poultry tissues. The method comprises the following steps of selecting a poultry sample, adding 
sodium chloride and 
acetonitrile, shaking out, adding 
sodium hydroxide solution, and vibrating for 30s to 5min; then, ultrasonic extracting for 10 to 40min, centrifuging, absorbing supernate for standby use, 
drying other solution by 
distillation, adding concentrated 
hydrochloric acid in the residues, adjusting pH to be about 1 to 3; then, adding 
acetonitrile, vibration extracting, centrifuging solution subjected to 
acetonitrile vibration extracting, absorbing supernate to merge with the above supernate, concentrating to dry to obtain sample extracting solution residues; adding a matrix 
solid-phase dispersion agent and 2ml 
ethyl acetate, swirling for 2min, taking supernatant liquid, 
drying through 
nitrogen, then adding 30 percent of 
methanol solution containing 0.1 percent of methanoic acid, ultrasonic dissolving, filming and injecting the sample; adopting HLPC-MS to carry out 
gradient elution detection. According to the method provided by the invention, the current standard one-time one-sample one-item is adjusted to one-time one-sample five-items, so that the time consumed for detection is greatly shortened.