199results about How to "Sensitive and accurate detection" patented technology

Molecular recognition-based electronic sensor, which is gateless, depletion mode field effect transistor consisting of source and drain diffusions, a depletion-mode implant, and insulating layer chemically modified by immobilized molecular receptors that enables miniaturized label-free molecular detection amenable to high-density array formats. The conductivity of the active channel modulates current flow through the active channel when a voltage is applied between the source and drain diffusions. The conductivity of the active channel is determined by the potential of the sample solution in which the device is immersed and the device-solution interfacial capacitance. The conductivity of the active channel modulates current flow through the active channel when a voltage is applied between the source and drain diffusions. The interfacial capacitance is determined by the extent of occupancy of the immobilized receptor molecules by target molecules. Target molecules can be either charged or uncharged. Change in interfacial capacitance upon target molecule binding results in modulation of an externally supplied current through the channel.

Molecular recognition-based electronic sensor, which is gateless, depletion mode field effect transistor consisting of source and drain diffusions, a depletion-mode implant, and insulating layer chemically modified by immobilized molecular receptors that enables miniaturized label-free molecular detection amenable to high-density array formats. The conductivity of the active channel modulates current flow through the active channel when a voltage is applied between the source and drain diffusions. The conductivity of the active channel is determined by the potential of the sample solution in which the device is immersed and the device-solution interfacial capacitance. The conductivity of the active channel modulates current flow through the active channel when a voltage is applied between the source and drain diffusions. The interfacial capacitance is determined by the extent of occupancy of the immobilized receptor molecules by target molecules. Target molecules can be either charged or uncharged. Change in interfacial capacitance upon target molecule binding results in modulation of an externally supplied current through the channel.

An improved imaging apparatus is disclosed that allows a user to perform numerous imaging operations. The imaging apparatus may include one or more improvements to imaging box design to improve illumination control within the imaging box, such as improved door seal arrangements, improved door closing mechanisms, and improved light seals. The present invention may also include one or more improvements to imaging apparatus design to facilitate image capture, such as: an automated filter select device, a moveable stage, automated focus control, f-stop adjustment and stage height, and improved internal illumination for capturing photographic images.

The invention relates to a cyclic RNA molecular marker for diagnosis of gastric cancer, the cyclic RNA molecular marker is characterized in that the cyclic RNA is hsa-circ-0001017, the invention also provides a method for detection of the cyclic RNA molecular marker in plasma, and the method comprises the following steps: (1) collecting blood, and extracting total RNA in the plasma; (2) performing reverse transcription of the total RNA into cDNA; (3) performing droplet digital PCR detection of a cDNA solution of the step (2) by use of specific amplification back-to-back primers and amplification upstream and downstream primers of housekeeping gene GAPDH, after the completion of the reaction, detecting fluorescence signal values of all droplets, setting a threshold, and determining whether the droplets include the cyclic RNA or the housekeeping gene GAPDH, wherein the droplets higher than the threshold are positive droplets, and the droplets below the threshold are negative droplets; and (4) counting the number of the positive droplets, and calculating the copy number of the hsa-circ-0001017 and the housekeeping gene GAPDH in the plasma for quantitative detection of the hsa-circ-0001017 and the housekeeping gene GAPDH in the plasma. Compared with the prior art, the advantages are that the hsa-circ-0001017 can be specifically expressed in plasma in patients with gastric cancer, and can be used as a new molecular marker for diagnosis of the gastric cancer.

The invention discloses a nuclear power plant pipeline leakage detection device and method based on distributed optical fiber temperature measurement. The nuclear power plant pipeline leakage detection device comprises a detection sensor unit, a signal receiving and transmitting processing unit and a data analysis and alarm unit. The detection sensor unit comprises an optical fiber and a fastening support, wherein the optical fiber is fixed to the portion above a high-energy pipeline through the fastening support. In the signal receiving and transmitting processing unit, a laser drive device is connected with the optical fiber through a wavelength division demultiplexer, the optical fiber is connected with the input end of a photoelectric detector through the wavelength division demultiplexer, and the output end of the photoelectric detector is connected with the input end of a signal processor. The data analysis and alarm unit comprises a PC terminal and an alarm indicator, wherein the output end of the signal processor is connected with the PC terminal, and the alarm indicator is connected with the PC terminal. The situation of high-energy pipeline system leakage in nuclear islands can be found in time, the leakage positions can be accurately positioned, the implementation foundation is provided for the leak-before-break design of the high-energy pipeline, and the safety of nuclear power plants is improved.

According to the present invention, there is provided a high-precision differential pressure sensor which is not affected by a considerable change in baseline pressure. A differential pressure sensor of the present invention includes: a pair of diaphragms, each including a diaphragm portion capable of being deformed due to application of a pressure and a support portion for holding an outer peripheral edge of the diaphragm portion; a pair of fixed electrodes in disk-like form fixed to the support portions of the diaphragms; and a movable electrode including a disk-like electrode portion and shaft-like projections extending in opposite directions from a central portion of the electrode portion. The shaft-like projections extend at a right angle relative to the electrode portion, and the movable electrode is secured to central portions of the diaphragms through the shaft-like projections so that the electrode portion faces each of the fixed electrodes in a predetermined spaced relationship. The movable electrode is capable of moving so as to allow a distance between the electrode portion and each of the fixed electrodes to vary according to a difference between fluid pressures acting on the respective diaphragm portions of the diaphragms. A capacitance generated between the electrode portion and each of the fixed electrode changes due to a variance in the distance between the electrode portion and each of the fixed electrode, and a differential pressure is detected, based on this change in capacitance.

The invention provides a nucleic acid aptamer fluorescence test strip, a method for preparing the same and application of the nucleic acid aptamer fluorescence test strip, and relates to the technicalfield of biological detection. The nucleic acid aptamer fluorescence test strip comprises a bottom plate, a sample pad, a nanometer material pad, a reaction film and a water absorption pad. The reaction film is provided with a detection line, and the detection line is arranged at the end, which is close to the nanometer material pad, of the reaction film; nucleic acid aptamer with fluorescein labels and / or nucleic acid aptamer with fluorescence labels and linker sequences and free fluorescein are attached to the nanometer material pad, and complementary strains of nucleic acid aptamer or complementary strains of linker sequences of nucleic acid aptamer are fixed to the detection line. The method for preparing the nucleic acid aptamer fluorescence test strip includes sequentially adheringthe sample pad, the nanometer material pad, the reaction film and the water absorption pad on the same side of the bottom plate. The nucleic acid aptamer fluorescence test strip, the method and the application have the advantages that the nucleic acid aptamer fluorescence test strip is simple in structure, sensitive in detection and high in accuracy; the method is easy to implement and high in flexibility.

The invention provides a multiplex PCR primer detecting gene fusion through combination of anchored nested multiplex PCR and high-throughput sequencing. The multiplex PCR primer comprises a multiplex PCR specific primer group 1 and a multiplex PCR specific primer group 2, wherein the multiplex PCR specific primer group 1 consists of nucleotide sequences shown in SEQ ID NO:1-SEQ ID NO:32, and the multiplex PCR specific primer group 2 consists of nucleotide sequences shown in SEQ ID NO:33-SEQ ID NO:64. The invention further provides a method for detecting gene fusion through combination of anchored nested multiplex PCR and high-throughput sequencing. The detection method is high in universality and can be used for detecting fusion genes accurately and sensitively under the condition that structures of fusion genes are partially known or unknown.

The invention provides a spin microwave oscillator and a spin microwave detector. The spin microwave oscillator of the invention is directly controlled by direct current based on microwave oscillationand output of a spin angle momentum transfer principle, or jointly regulated and controlled by direct current and impressed static magnetic field, has the advantages of miniaturization, high integration, low power consumption, high controllability and tunability and the like. The spin microwave detector of the invention directly detects input microwave by direct voltage generated by the input microwave, and has the advantage of direct measurement on high-frequency signals in no need of hybrid frequency.

The invention provides an ELISA (enzyme-linked immuno sorbent assay) detection kit for analyzing residual carbaryl, which comprises a kit body, a dismountable ELISA plate arranged in the kit body and a reagent arranged in the kit body, wherein every hole of the ELISA plate is coated with carbaryl coating antigen, the reagent comprises an anti-carbaryl nano antibody, a carbaryl standard solution, a second antibody of an enzyme marker, a buffer solution PBS, a scrubbing solution PBST, substrate liquid, a developing solution, a reaction stop solution and the like. In the detection process, the coating antigen adsorbed on a hole wall of the ELISA plate and the carbaryl to be detected compete with each other to react with the antibody, and the competitive result is shown through chromogenic reaction. By detecting the carbaryl with known concentration, and drawing a standard curve, the concentration of carbaryl to be detected can be calculated. The ELISA detection kit provided by the invention has the advantages that the residual carbaryl in water, soil and vegetables can be accurately and sensitively detected, the pretreatment process of a sample is simple and consumes short time, a large quantity of samples can be detected simultaneously, and the sample detection cost is far lower than that of a traditional instrument detection method.

An improved imaging apparatus is disclosed that allows a user to perform numerous imaging operations. The imaging apparatus may include one or more improvements to imaging box design to improve illumination control within the imaging box, such as improved door seal arrangements, improved door closing mechanisms, and improved light seals. The present invention may also include one or more improvements to imaging apparatus design to facilitate image capture, such as: an automated filter select device, a moveable stage, automated focus control, f-stop adjustment and stage height, and improved internal illumination for capturing photographic images.

The invention relates to a method for detecting an insulating resistance and releasing static electricity and a device thereof. An insulating detection point is set in each position of detected equipment which needs the insulating resistance detection; a direct current power supply is arranged outside the detected equipment; direct current with certain voltage is generated through the direct current power supply and is transported to the insulating detection point set in each position of the detected equipment which needs the insulating resistance detection; simultaneously, a voltage and current detection device is arranged in the middle of a direct current loop; the change of the direct current voltage and current of the insulating detection point set in each position of the detected equipment which needs the insulating resistance detection is detected and compared through the detection device; a monitoring device carries out voltage and current calculation to obtain the insulating resistance of the insulating point, judges whether the insulating resistance of the insulating point is normal and forewarns or warns according to the detected insulating resistance; simultaneously, according to the monitoring device, the output static voltage is determined, whether static release is needed is judged, and under the condition of needing static release, the static voltage is released for passing through a resistance network.

Provided herein are imaging cassettes for detecting a luminescent and / or radioactive signals. Such cassettes are useful in common biological assays, e.g., immunoassays, nucleotide detection assays, and other affinity assays.

The invention discloses a preparation method of a water-soluble nitrogen-phosphorus-boron codoped carbon quantum dot. The preparation method comprises the following steps: step I, dissolving phenylphosphinic acid in ultra pure water to obtain a precursor solution I, dissolving 3-aminobenzene boronic acid in ultra pure water to obtain a precursor solution II, and uniformly mixing the precursor solution I with the precursor solution II in the volume ratio of the precursor solution I to the precursor solution II being (1-6) to 1 so as to obtain a mixed solution; step II, performing a hydrothermal reaction on the mixed solution prepared in the step I so as to obtain the water-soluble nitrogen-phosphorus-boron codoped carbon quantum dot. According to the preparation method disclosed by the invention, the water-soluble nitrogen-phosphorus-boron codoped carbon quantum dot is prepared by the one-step hydrothermal method, carbon sources are rich, a preparation process is simple and easy, the whole preparation process is pollution-free, non-toxic, green and environmentally-friendly, and the water-soluble nitrogen-phosphorus-boron codoped carbon quantum dot can be prepared in a large-scale manner.

The invention discloses an axial force sensor, which comprises an elastic body, wherein the elastic body is of a flat plate shape; an axis penetration through hole is formed in the middle of the plate surface; a plurality of bolt penetration holes are formed at the periphery of the elastic body; the whole shape of the elastic body is similar to that of a gasket of a flange disk; an annular stress slot concentric with the shaft axis penetration through hole is formed on each of the front plate surface and the rear plate surface of the elastic body, and the diameters of the annular stress slots are equal substantially; the stress slots are close to the axis penetration through hole; resistance strain gauges are attached to the stress slots and form a WheatStone balance bridge which is connected with an external electric appliance. The axial force sensor is low in height, small in size and similar to the gasket in the shape, can be conveniently assembled between the force applying shaft of the appliance and the flange disk or a stressed part at the connection shaft end of the appliance, is damaged difficultly, and is firm and high in lateral and unbalance loading resistance; and when the sensor is stressed by an axial load, part of the elastic body in each stress slot is effectively deformed, so that an axial force value can be sensitively and accurately detected.

The invention discloses a method of detecting the content of a preservative in a cosmetic, and the method comprises the following steps of (1) treating a sample to be detected; (2) preparing a preservative standard product stock solution and a standard series; (3) detecting by using a high performance liquid chromatography; (4) drawing a standard curve of a preservative standard mixed solution series, performing linear regression on the peak area of a preservative and the corresponding mass concentration, and calculating the content of the preservative in the sample to be detected according to an obtained equation of linear regression.

The invention discloses a brucella abortus indirect enzyme linked immunosorbent assay (ELISA) detection kit which consists of the following reagents: (1) a pre-enveloped ELISA reaction plate comprising enveloping liquid, an enveloping antigen and closing liquid, (2) plumbous stearate (PBST) washing liquid, (3) IgG-HRP, (4) ending liquid, and (5) color developing liquid, wherein the enveloping antigen is a Virb8 protein. The Virb8 protein related by the invention can be only specifically expressed in the early stage of the brucella abortus infection; an antibody correspondingly produced by the protein is in the early stage of the infection; the produced antibody can live for a long time, so that whether an antibody titer caused by the brucella abortus infection and the quantitative infection exists can be specifically and sensitively judged by cloning and expressing the brucella abortus Virb8 protein and constructing a corresponding indirect ELISA detection method; a quick and accurate method can be supplied to early serologic diagnosis of the brucella abortus disease; the brucella abortus indirect ELISA detection kit has a great practical significance for a brucella abortus site detection technology for a large batch of samples.

The invention discloses an assay kit used for detecting human papillomavirus, comprising human papillomavirus sequencing primer solution. The invention also discloses a preparation method and use method of the kit used for detecting high-risk type human papillomavirus. The kit of the invention can determine whether a sample to be detected contains HPV virus and concrete virus subtype. Identity label used for discriminating sample source is added on a DNA sample to be detected, thus meeting high throughout visitation requirement of detecting a plurality of persons at once. The kit of the invention exceeds the traditional detection means, sequence differential is positioned to basic group level, detection is more sensitive and accurate, and meanwhile probability of false positive pollution is avoided.

The invention discloses an enzyme-linked immunosorbent assay method for multi-residue analysis of a cyano-containing pyrethroid pesticide, belonging to the technical field of immunoassay. The enzyme-linked immunosorbent assay method uses a coupled complex of hapten m-phenoxy benzal cyanohydrin succinate ester and ovalbumin as a coating antigen, samples of the cyano-containing pyrethroid and a polyclonal antibody of the m-phenoxy benzal cyanohydrin succinate ester are added, then the coating antigen and the cyano-containing pyrethroid to be detected are competed and reacted with the antibody, an enzyme-labeled secondary antibody is added to be combined with the fixed antibody, washing liquid is used for washing, developer is added, an enzyme-labeled instrument is used for detecting OD value, the OD value is compared with a standard curve of a standard product of the cyano-containing pyrethroid, and the concentration of the cyano-containing pyrethroid of the samples to be detected is calculated. The enzyme-linked immunosorbent assay method can accurately and sensitively detect the residues of the cyano-containing pyrethroid pesticide in the samples to be detected, the pre-treatment process of the samples is simple with less time-consuming, a large number of samples can be simultaneously detected, and the detection cost of the samples is far lower than the detection method of the traditional instrument; furthermore, the method of the invention has good stability and no radioactive pollution.

The invention discloses a PCR (polymerase chain reaction) synchronous detection primer for staphylococcus aureus enterotoxin A and B genes, a detection kit thereof and a detection method. The nucleotide sequence of the synchronous detection primer is as shown in SEQ (sequence) ID (identity) No. 1 and 2. The invention further provides the PCR detection kit containing the primer. By adopting the detection kit, the staphylococcus aureus enterotoxin A and B in a food can be accurately and sensitively detected, and the lowest detection concentration of DNA (deoxyribonucleic acid) is 3.58ng; furthermore, the detection kit has no cross reaction with other bacteria, and the specificity is good; simultaneously, the pretreatment process of samples is simple, the consumed time is short, a large number of the samples can be detected simultaneously, and the cost is low.

The invention relates to a method for rapid fluorescence detection of polynucleotide target objects simultaneously at room temperature and constant temperature. The method comprises the steps that at the constant temperature, single-chain binding proteins partially open parental chains of double chains of multiple templates to form single chains; recombinases are bonded with multiple pairs of primers to form complexes which are bonded to the parental chains under the action of accessory proteins, and multiple fluorescence probes are also bonded with a complementation region; DNA polymerases are bonded to the 3' terminals of the primers so as to perform subchain extension; exonucleases recognize tetrahydrofuran sites on the multiple fluorescence probes which are under the double chain condition, so that fluorophores and quenching groups are separated after digestion, and fluorescence is released; after the multiple fluorescence probes are cut, 3'-OH ends which are originally closed due to modification of the 3' terminals of the probes are exposed, and the DNA polymerases can further extend to form subchains; to-be-tested samples can be subjected to qualitative and semi-quantitative determination through detecting the shape of amplification curves and the strength of fluorescence signals. The method disclosed by the invention can be used for qualitative and semi-quantitative determination of multiple target objects simultaneously at room temperature and constant temperature.

The invention discloses a preparation method of water-soluble nitrogen-doped carbon quantum dots, comprising the following steps: Step 1, dissolving citric acid in ultrapure water to prepare precursor solution I, dissolving p-aminobenzamide in Prepare precursor solution II in pure water, uniformly mix precursor solution I and precursor solution II at a volume ratio of 1 to 10:1 to obtain a mixed solution; step 2, perform hydrothermal reaction on the mixed solution prepared in step 1, Water-soluble nitrogen-doped carbon quantum dots can be obtained. The invention adopts a one-step hydrothermal method to prepare water-soluble nitrogen-doped carbon quantum dots. The carbon source is rich and cheap, the preparation process is simple, the whole preparation process is pollution-free, non-toxic, green and environmentally friendly, and can be prepared in large quantities.

The invention discloses a method for analyzing and detecting trace ammonia nitrogen in complicated matrix by combining a fast distillation method with ion chromatography technology. The method comprises the following steps: firstly, carrying out distillation pretreatment on a complicated sample to enrich and extract ammonium ions, and then detecting by using an ion chromatography method. The sample pretreatment refers to extraction of ammonium ions from the sample by using a fast distillation method, and concretely comprises the following steps: firstly adding excessive base into the sample, heating and boiling so as to release ammonium ions in a manner of ammonia gas without carrying other interference ions, and absorbing ammonia gas at an absorption part with excessive acid, thus obtain purified ammonium ions. The extracted ammonium is detected by using the ion chromatography method. The method is excellent in the ammonium extraction effect, capable of eliminating the interference of sodium ions, potassium ions and other anodic ions to the ammonium detection, convenient and sensitive, and fast and accurate.

According to the present invention, there is provided a high-precision differential pressure sensor which is not affected by a considerable change in baseline pressure. A differential pressure sensor of the present invention comprises: a pair of diaphragms, each including a diaphragm portion capable of being deformed due to application of a pressure and a support portion for holding an outer peripheral edge of the diaphragm portion; a pair of fixed electrodes in disk-like form fixed to the support portions of the diaphragms; and a movable electrode including a disk-like electrode portion and shaft-like projections extending in opposite directions from a central portion of the electrode portion. The shaft-like projections extend at a right angle relative to the electrode portion, and the movable electrode is secured to central portions of the diaphragms through the shaft-like projections so that the electrode portion faces each of the fixed electrodes in a predetermined spaced relationship. The movable electrode is capable of moving so as to allow a distance between the electrode portion and each of the fixed electrodes to vary according to a difference between fluid pressures acting on the respective diaphragm portions of the diaphragms. A capacitance generated between the electrode portion and each of the fixed electrode changes due to a variance in the distance between the electrode portion and each of the fixed electrode, and a differential pressure is detected, based on this change in capacitance.

The invention discloses an LED light source short-circuit detection method and device, an LED backlight and a liquid crystal display device. The LED light source short-circuit detection method and device aims to simplify the short-circuit detection of an existing LED light source. The LED light source short-circuit detection method is applied to the backlight of the liquid crystal display device, wherein the backlight comprises a plurality of LED lamp strips. The LED light source short-circuit detection method comprises the following steps of inputting M unequal detection voltages to each LED lamp strip; receiving and recording breakover feedback of each LED lamp strip at under each detection voltage; judging whether LED lamps are short-circuited in each LED lamp strip according to the breakover feedback, wherein M is an integer which is not smaller than 1, and the maximum detection voltage is not larger than the normal working voltage of each LED lamp strip. The LED light source short-circuit detection method and device is used for the short-circuit detection of the LED light source, and has the advantages of being easy and convenient to achieve, fast in achievement, sensitive in detection and the like.

This inventon shows a reagent box , which is used for enzyme linked immunosorbent assay of remained carbaryl. It belongs to enzyme linked immunosorbent assay technology. The common methods of carbaryl rationalize analyze are too complex, slow and its cost is high. This invention overcomes these defaults. It can quickly detect the remained carbaryl in water, soil and food samples. In the process, the antigen on the enzyme target rivals carbaryl . It reacts with the antigens in the solution. And the result is showed by enzyme catalysis color reaction. You can estimate the consistency of carbaryl by detecting the solution, whose consistency of carbaryl is known and drawing calibration vurve. The retention period of the box is more than 6 months.

The invention belongs to the field of biological detection and particularly relates to an A-type foot-and-mouth disease competition ELISA antibody detection kit. By adopting the kit to improve the original in-vitro reaction process, the reaction time is greatly shortened from the original 4 h to 1.5 h, the original accessories of the in-vitro reaction are saved, and the kit is more rapid and economic. The kit adopts an enzyme-labeled antibody to carry out reaction, so that compared with the traditional enzyme-labeled secondary antibody process, the detection is more correct and sensitive. The reaction plate coated by using the method provided by the invention is strong in adsorbability and is capable of effectively promoting the correctness of the detection.

The invention discloses a preparation method and application of a double-color fluorescent gold-copper composite nano-cluster. The double-color fluorescent gold-copper composite nano-cluster is prepared from the raw materials, namely a chloroauric acid solution, a copper nitrate solution and glutathione by virtue of an oil-bath heating method. The double-color fluorescent gold-copper composite nano-cluster has the double-color fluorescent properties of emitting blue fluorescence (450nm) and orange red fluorescence (570nm) under the stimulation of a single wavelength, the blue fluorescence is hardly influenced by metal ions, and the intensity of the orange red fluorescence can be decreased or even the orange red fluorescence can be quenched by virtue of mercury ions and mercury compounds. Based on the characteristic, a fluorescence detection technique for detecting the mercury ions and the mercury compounds by virtue of a rate type fluorescence probe is constructed. The preparation method is simple, convenient and low in cost, the double-color fluorescent gold-copper composite nano-cluster has excellent fluorescent and chemical stabilities, and the disadvantages that a traditional dye is short in fluorescent lifetime, easy to photobleach and the like can be overcome; and a rate type fluorescent method is high in sensitivity and good in stability, does not require expensive instrument, can be applied to environmental monitoring and analysis and has wide application prospects.

The invention discloses a method for detecting acute toxicity of rare earth tailing pond surrounding groundwater pollution by using freshwater luminescent bacteria. The freshwater luminescent bacteria are Vibrio qinghaiensis Q67; the method comprises steps as follows: a to-be-detected sample is treated and diluted, and the pH value is adjusted; an activated Vibrio qinghaiensis Q67 solution is added to the to-be-detected sample for a reaction; meanwhile, a diluent for dilution replaces the to-be-detected sample to serve as a contrast; the luminescent intensity of the to-be-detected sample and the luminescent intensity of the contrast are detected by a bioluminescence tester; the result is calculated, standard reference poison ZnSO4 is set, and the toxicity effect is judged according to the result. The method is rapid, sensitive, accurate and convenient to popularize and apply. Meanwhile, Vibrio qinghaiensis, the freshwater luminescent bacteria, is used as a test strain, and the influence caused by salinity required by marine luminescent bacteria on luminescence is avoided. The reference poison is ZnSO4, and the influence on the ecological environment and human health is reduced.

The invention discloses an inhibin B enzyme-linked immunosorbent assay kit and an inhibin B detection method. The inhibin B enzyme-linked immunosorbent assay kit includes an ELISA plate coated with an anti inhibin B beta-subunit antibody, a biotin-labeled secondary antibody, and an avidin-labeled enzyme marker; a coating antigen used for preparation of the ELISA plate coated with the anti inhibin B beta-subunit antibody is a specific antibody aiming at an inhibitor B beta subunit; the biotin labeled secondary antibody is a biotin-labeled inhibin B alpha-subunit monoclonal antibody. The inhibin B detection method comprises that the inhibin B enzyme-linked immunosorbent assay kit is used for detection of the inhibin B. Therefore, the inhibin B enzyme-linked immunosorbent assay kit and the inhibin B detection method have the characteristics of high sensitivity, high specificity, time saving and the like, and can be applied to clinical medical batch detection of the inhibin B.