36results about How to "Strong target specificity" patented technology

The invention provides a construction method of LRFFT2 cells; human peripheral blood is used for ctDNA sequencing or tumor tissues are used for full exon sequencing, mutant sites are screened out forepitope prediction and are connected to synthesize a mutant polypeptide expression gene sequence; at the same time, a lentivirus vector is constructed, lentiviruses are packaged, APC cells are transfected, the transformation of specific LV cells is completed, the transformed specific LV cells are co-cultured with PBMC isolated from the peripheral blood in vitro, and effective polypeptides are screened out; through precise effective second shocking stimulated by the polypeptides, ordinary T cells are transformed into RFF cells with more precise killing ability; transformation is preformed by aTCR-T technical principle; the transformed T cells are subjected to immunosuppressive signal sealing with antibody drugs in vitro, the specific killer T cells are precisely protected from inhibition in vivo, the killing ability of the T cells to tumor cells is improved, and the LRFFT2 cells with both attack and defense are obtained. The LRFFT2 cells can be widely applied in individualized and precise treatment of solid tumors.

Patient peripheral blood is used for ctDNA sequencing or tumor tissue is used for whole exon sequencing, mutation sites are screened to be subjected to epitope presequencing, and connection and synthesis are performed to obtain a mutation polypeptide expression gene order. Besides, a retroviral vector is constructed, slow viruses are packed, APC cells are transfected, and reformation of specificity LV cells is completed. PBMC obtained by in vitro and peripheral blood separation is cocultured, effective pinpoint polypeptide is screened, and through second impact of pinpoint polypeptide stimulation, common T cells are reformed to obtain LRFF cells having pinpoint killing capacity, and the killing capacity of the T cells to tumor cells can be improved. The LRFF provided by the invention can be widely applied to individual pinpoint treatment of solid tumors.

The application relates to AFFT1 cell and a preparation method thereof. The RFF cell is reformed by using TCR-T technical principle, the knock-out of the immunosuppression target spot is performed onthe reformed T cell by using gene editing technology, the condition that the specific lysis T cell is prevented from in-vivo suppression is precisely protected, and the lethality on the tumor cell bythe T cell is improved.