The invention relates to a 
water quality integrated bio-
toxicity on-line automatic analyzer and a method for using the same. The analyzer is characterized in that the interior of the analyzer is provided with 
two temperature control areas, wherein one temperature area at a temperature of between 0 and 4 DEG C mainly comprises a freezing 
dry powder storing device, a sterilizing device, a freezing 
dry powder definite quantity control device and a 
nutrient solution storing device; and the other temperature area at a temperature of between 20 and 25 DEG C mainly comprises a photogenic bacterium activating reaction storing kettle, a buffering liquid storing device, a regulation and control device for water to be tested, a clear 
water flow rate transmission and control pipeline, a delicate testing device and a rotary long shaft fixing round plate in a dark box, wherein a 
test tube is assembled on the round plate; the dark box cover is connected with the photogenic bacterium activating reaction storing kettle, the buffering liquid storing device, the control device for water to be tested and an analyzer externally connected washing 
pipe through the flow rate transmission and control pipeline; and the interior of the dark box is provided with a photoelectric probe. The analyzer has the advantages of on-line test achievement for 
water quality integrated bio-
toxicity, simple operation and high 
automation degree in the aspect of maintenance, and can obtain reasonable 
cost performance and be suitable for the on-line monitoring of abominable environment. The analyzer can be used as a single 
machine, and can also be used as a front-end product of a monitoring network 
system.