Biocontrol bacteria compound agent for preventing and controlling crop soil-infectious diseases and preparation method

[0019] Implementation example 1: get 10 grams of crop rhizosphere soil, add sterile water to shake and then gradiently dilute to 10,000 times to make soil suspension, get 1 milliliter of this suspension and add 9 milliliters of PDA medium containing streptomycin sulfate (potato 200 grams of glucose, 20 grams of agar, 20 grams of agar, and 1000 ml of water) to make a plate, culture at 26°C for 48 hours, obtain Trichoderma viride (Trichoderma viride) and Bacillus subtilis (Bacillus subtilis) after separation and purification, and transfer to a test tube The slant is used as the mother fungus for preservation.

[0020] Subtilis and Trichoderma viride used in the medium formulations of the secondary strain fermentation broth are: yeast extract 5 grams, 30 grams of sucrose, 1000 milliliters of water. The production method is as follows: weigh 5 grams of yeast extract and 30 grams of sucrose in a large beaker, add a small amount of water and heat to dissolve completely, then add wat...

[0023] Implementation example 2: get 10 grams of crop rhizosphere soil, add sterile water to shake and then gradiently dilute to 10,000 times to make soil suspension, get 1 milliliter of this suspension and add 9 milliliters of PDA medium containing streptomycin sulfate (potato 200 grams of glucose, 20 grams of agar, 20 grams of agar, and 1000 ml of water) to make a plate, culture at 26°C for 48 hours, obtain Trichoderma viride (Trichoderma viride) and Bacillus subtilis (Bacillus subtilis) after separation and purification, and transfer to a test tube The slant is used as the mother fungus for preservation.

[0024] Subtilis and Trichoderma viride used in the medium formulations of the secondary strain fermentation broth are: yeast extract 5 grams, 30 grams of sucrose, 1000 milliliters of water. The production method is as follows: weigh 5 grams of yeast extract and 30 grams of sucrose in a large beaker, add a small amount of water and heat to dissolve completely, then add wat...

[0028] Implementation example 3: get 10 grams of crop rhizosphere soil, add sterile water to shake and then gradiently dilute to 10,000 times to make soil suspension, get 1 milliliter of this suspension and add 9 milliliters of PDA medium containing streptomycin sulfate (potato 200 grams of glucose, 20 grams of agar, 20 grams of agar, and 1000 ml of water) to make a plate, culture at 26°C for 48 hours, obtain Trichoderma viride (Trichoderma viride) and Bacillus subtilis (Bacillus subtilis) after separation and purification, and transfer to a test tube The slant is used as the mother fungus for preservation.

[0029] The formula of the medium for the fermentation broth of the secondary strain is: 5 grams of yeast extract, 30 grams of sucrose, and 1000 milliliters of water. The production method is as follows: weigh 5 grams of yeast extract and 30 grams of sucrose in a large beaker, add a small amount of water and heat to dissolve completely, then add water to 1000 ml. The ster...