D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration

[0100] The D-lactic acid diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0101] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0102] Stabilizer 500mmol / L

[0103] Reduced coenzyme 0.25mmol / L

[0104] 2 Ferricytochrome c 10mmol / L

[0105] Ammonia ion 20mmol / L

[0106] D-lactate dehydrogenase 10000U / L

[0107] Alanine dehydrogenase 10000U / L

[0108] Glycine oxidase 10000U / L

[0109] Peroxidase 30000U / L

[0110] 4-amino antiarsenic 2mmol / L

[0111] Carbolic acid 10mmol / L

[0112] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0113] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, the volume ratio of the measured D-lactic acid sample to the reagent is 1 / 25, and the reaction direction is...

[0116] The D-lactic acid diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0117] Reagent 1

[0118] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0119] Stabilizer 50mmol / L

[0120] Reduced coenzyme 0.25mmol / L

[0121] 2 Ferricytochrome c 10mmol / L

[0122] Ammonia ion 20mmol / L

[0123] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0124] Reagent 2

[0125] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0126] Stabilizer 500mmol / L

[0127] D-lactate dehydrogenase 10000U / L

[0128] Alanine dehydrogenase 10000U / L

[0129] Glycine oxidase 10000U / L

[0130] Peroxidase 25000U / L

[0131] 4-amino antiarsenic 0.6mmol / L

[0132] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0133] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 54...

[0136] The D-lactic acid diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0137] Reagent 1

[0138] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0139] Stabilizer 50mmol / L

[0140] Reduced coenzyme 0.25mmol / L

[0141] 2 Ferricytochrome c 10mmol / L

[0142] Ammonia ion 20mmol / L

[0143] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0144] Reagent 2

[0145] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0146] Stabilizer 500mmol / L

[0147] Alanine dehydrogenase 10000U / L

[0148] Glycine oxidase 10000U / L

[0149] Peroxidase 20000U / L

[0150] Dimethylaniline 2mmol / L

[0151] Reagent 3

[0152] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0153]Stabilizer 500mmol / L

[0154] D-lactate dehydrogenase 10000U / L

[0155] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

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