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100 results about "Alanine dehydrogenase" patented technology
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Alanine dehydrogenase (EC 1.4.1.1) is an enzyme that catalyzes the chemical reaction L-alanine + H₂O + NAD⁺ ⇌ pyruvate + NH₃ + NADH + H⁺ The 2 substrates of this enzyme are L-alanine, water, and nicotinamide adenine dinucleotide⁺ because water is 55M and does not change, whereas its 4 products are pyruvate, ammonia, NADH, and hydrogen ion. This enzyme participates in taurine and hypotaurine metabolism and reductive carboxylate cycle (CO2 fixation).
Escherichia coli for efficiently producing L-alanine by fermentation
ActiveCN104774790AAbility to produce high L-alanineHigh chemical purityBacteriaMicroorganism based processesEscherichia coliSuccinic acid
The invention discloses escherichia coli for efficiently producing L-alanine by fermentation, and belongs to the technical field of microbial metabolic engineering. Coding genes for a synthesis route for byproducts acetic acid, formic acid, alcohol, succinic acid and lactic acid on a chromosome of escherichia coli CGMCC No.10628 provided by the invention are deleted, and the chromosome dadX gene is replaced as an alanine dehydrogenase gene, wherein the coding genes comprise ackA-pta, pflB, adhE, frdA and idhA. The recombinant bacterium is fermented for 26 hours to produce 106g / L or more than 106g / L L-alanine with high optical purity and high chemical purity at 28-45 DEG C; and in the whole fermentation process, the production strength reaches 4.27g / L.h or more.
Owner:JIANGNAN UNIV
Method for synthesizing L-2-aminobutyric acid by enzymatic method
ActiveCN107012178ANo effectSuitable for industrial productionFermentationHydrogenL-2-Aminobutyric Acid
The invention discloses a method for synthesizing L-2-aminobutyric acid by an enzymatic method. According to the method, 2-ketobutyric acid is catalyzed by the aid of alanine dehydrogenase and formate dehydrogenase to produce the L-2-aminobutyric acid. The method particularly includes the steps: uniformly mixing 0.5-1.5mol of 2-ketobutyric acid, 0.5-1.5mol of ammonium formate, o-0.5g / L of NAD (nicotinamide adenine dinucleotide) and 20g / L of formate dehydrogenase and alanine dehydrogenase co-expression wet cells (or 10-30g / L of formate dehydrogenase wet cells, 10-30g / L of alanine dehydrogenase wet cells) in a rector; adjusting a pH (potential of hydrogen) value to be 7.0-9.0; performing catalytic reaction for 16-20h at the temperature ranging from 30 DEG C to 37 DEG C to obtain the L-2-aminobutyric acid. The method is rapid in reaction, reverse oxidation deamination activity is low, and product loss is greatly reduced.
Owner:SHANDONG YANGCHENG BIOLOGY TECH CO LTD
Trigenic coexpression vector for synthesizing DL-alanine and application
ActiveCN107794273AImprove conversion efficiencyBacteriaMicroorganism based processesBacillus coliAlanine racemase
The invention discloses construction of a trigenic coexpression vector for synthesizing DL-alanine and application. The construction is characterized in that encoding alanine dehydrogenase (ald), alanine racemase (alr) and glucose dehydrogenase (gdh) genes in pseud bacillus firmus are connected in series and then are inserted into reconstructed plasmid pET-22bNS, so as to construct the trigenic coexpression vector pET-22bNS-G / A / A. After constructed trigenic coexpression vector is transformed into escherichia coli BL21(DE3) and is subjected to whole-cell catalytic reaction for 3h through recombination strain, the output of L-alanine and the output of D-alanine are the highest and are correspondingly 7.0mg / mL and 6.5mg / mL, and the maximum synthesizing efficiency of L-alanine and the maximumsynthesizing efficiency of D-alanine are correspondingly 56.4mg / mL / d and 51.9mg / mL / d. The constructed trigenic coexpression vector has a capacity of efficiently synthesizing DL-alanine and is high inapplication value.
Owner:HEBEI NORMAL UNIV
Homocysteine diagnostic kit and method for measuring homocysteine concentration
InactiveCN101609017AMicrobiological testing/measurementColor/spectral properties measurementsLyaseWavelength
The invention relates to a homocysteine diagnostic kit utilizing technologies of an indirect enzymatic recycling method, an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring homocysteine concentration and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, pyruvic acid, reduced coenzyme, homocysteine-alpha, gamma-lyase, alanine dehydrogenase, glycine oxidase, peroxidase, reduced form chromogen composition, and stabilizer; the colorless reduced form chromogen composition is oxidized into colored dye through a series of enzymatic reactions; moreover, dye content can be measured at the position where wave length is between 400 and 700nm through a visible analyzer so as to directly reflect the homocysteine concentration.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
5'-UTR element and application thereof in production
ActiveCN106480035AVerify the transformation effectIncrease productionBacteriaMicroorganism based processesNucleotideA-DNA
The invention discloses a 5'-UTR element and an application thereof in production, in particular an application in L-alanine fermentation production. Firstly, the invention provides a DNA molecule A, which is shown as the 294th-n1st nucleotide of a sequence 14 in a sequence list, wherein n1 is a natural number which is greater than 310 and less than 606. The invention also discloses an application of the DNA molecule A, as a regulatory element, in promoting the expression of a target gene. The invention also discloses a DNA molecule C, wherein the DNA molecule C, from upstream to downstream, sequentially consists of the following elements: the DNA molecule A and a gene for encoding alanine dehydrogenase. The invention also discloses a recombinant bacterium C containing the DNA molecule C. The invention also discloses an application of the recombinant bacterium C in producing L-alanine. According to the invention, a nucleotide sequence, which can effectively enhance gene expression, is obtained, a strain for producing the alanine is constructed and a novel method for the improved alanine fermentation production is provided.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Method for realizing whole cell transformation to synthesize L-phenyllactic acid by genetic engineering strain
ActiveCN109593702ASolve the loop problemAdd lessBacteriaMicroorganism based processesPhenylalanine dehydrogenaseBio engineering
The invention discloses a method for realizing whole cell transformation to synthesize L-phenyllactic acid by a genetic engineering strain, in particular to a genetic engineering strain capable of co-expressing phenylalanine dehydrogenase and L-hydroxyisocaproic acid reductase to realize self-circulation of cofactors NAD<+> and NADH. The method for synthesizing L-phenyllactic acid by a whole celltransformation substrate L-phenylalanine belongs to the field of bioengineering technology. By using the whole cell transformation of a recombinant strain E.coli BL21(DE3) / pET28a-pdh-ldh to synthesizeL-phenyllactic acid, the whole cell transformation rate can reach 88.9% to 95.6% under the action of an added surfactant. By adopting the method, the self-circulation of the cofactors NAD<+> and NADHis realized, the addition amount of the cofactors is reduced, the production cost is reduced, and the method has a broad application prospect in the field of industrial synthesis of L-phenyllactic acid.
Owner:JIANGNAN UNIV
Alanine dehydrogenase mutant and application thereof in fermentation production of L-alanine
ActiveCN111748535AHigh activityGood yieldBacteriaMicroorganism based processesAlanine dehydrogenaseBiology
The invention discloses an alanine dehydrogenase mutant and an application of the alanine dehydrogenase mutant in fermentation production of L-alanine. The alanine dehydrogenase mutant disclosed by the invention is a protein obtained by mutating lysine at the 327th site of an amino acid sequence of alanine dehydrogenase into asparagine. According to the invention, the alanine dehydrogenase mutantwith significantly improved activity is obtained based on a metabolic domestication screening technology, and the alanine dehydrogenase mutant is used for construction of L-alanine engineering strainsand efficient fermentation of L-alanine. The experiments prove that the L-alanine yield and the enzymatic activity of the L-alanine dehydrogenase of the strain provided by the invention are greatly improved and are improved by 88.2% compared with those of a strain for expressing wild alanine dehydrogenase, the fermentation capability and the L-alanine yield of an L-alanine engineering strain canbe effectively improved, and the mutant has important application value.
Owner:ANHUI HUAHENG BIOTECH +1
Method for immobilization of phenylalanine dehydrogenase by chemical reduced graphene oxide
InactiveCN109456960ALess structural interferenceEnables controlled immobilizationMicroorganism based processesOxidoreductasesPhenylalanine dehydrogenaseBiocompatibility Testing
The invention discloses a method for immobilization of phenylalanine dehydrogenase by chemical reduced graphene oxide, and belongs to the technical field of immobilized enzyme. The chemical reduced graphene oxide is stable in structure, is good in biocompatibility, and is a good enzyme immobilization carrier. The surface of the chemical reduced graphene oxide with a sheet-layer structure is lack of groups such as an epoxy group, a carbonyl group and a carboxyl group, is great in load area, and has hydrophobicity. The immobilization of phenylalanine dehydrogenase by the chemical reduced graphene oxide mainly has a hydrophobic effect; and in an immobilization process, the degree of reduction and salt concentration of the chemical reduced graphene oxide have influences on immobilization of enzyme by the chemical reduced graphene oxide. The obtained immobilized phenylalanine dehydrogenase is obviously improved in pH stability and repeated use stability, and meanwhile, has relatively high enzyme activity recovery rate. The method disclosed by the invention has the advantages that the process is simple; the preparation conditions are mild; and the immobilization rate is high.
Owner:XIAMEN UNIV
Determination method of glycine and kit for determining glycine
InactiveCN102087265ASuitable for testingMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementBiotechnologyAssay
The invention relates to a determination method of glycine content by adopting an enzymatic recycling method, an enzymatic colorimetric method and an enzyme-linked immuno sorbent assay technology, and composition and components of a reagent, and the technical principle of the determination is based on serial catalytic reaction of glycine dehydrogenase, glycine oxidase and alanine dehydrogenase. The invention also relates to a kit for determining the glycine. The determination method has the advantages of high sensitivity and small error. Therefore, the determination method and the kit disclosed by the invention can be widely applied to food inspection.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration
InactiveCN101324561AMicrobiological testing/measurementColor/spectral properties measurementsFerricytochrome cPeroxidase
The invention relates to a kit for diagnosing / mensurating D-lactic acid by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA) based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, 2-ferricytochrome-c, ammonia ions, D-lactate dehydrogenase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by utilizing a visible light analyzer and further reflecting the concentration of the D-lactic acid directly.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Nocardia seriolae attenuated strain and preparation method and application thereof
ActiveCN112746050AAvoid infectionSimple manufacturing methodAntibacterial agentsBacterial antigen ingredientsGenetic engineeringAlanine dehydrogenase
The invention is applicable to the technical field of genetic engineering and provides a nocardia seriolae attenuated strain and a preparation method and application thereof. The nocardia seriolae attenuated strain is a nocardia seriolae strain containing alanine dehydrogenase gene deletion. The preparation method comprises the following steps of: connecting an upstream fragment and a downstream fragment of an alanine dehydrogenase gene into a vector to obtain a recombinant vector; taking a nocardia seriolae wild strain to prepare nocardia seriolae competent cells; performing electrotransformation on the nocardia seriolae competent cells by utilizing the recombinant vector to obtain electrotransformation bacterial liquid; and culturing and screening the electrotransformation bacterial liquid to obtain the nocardia seriolae attenuated strain. The nocardia seriolae attenuated strain provided by the invention can be used for preventing nocardiosis infection; after the alanine dehydrogenase gene is deleted by a gene knockout method, the pathogenicity of bacteria can be effectively reduced, but relatively good immunogenicity is still retained.
Owner:SHENZHEN INST OF GUANGDONG OCEAN UNIV +2
Ammonia (ammonia ion) determination method and ammonia (ammonia ion) diagnosis/measurement kit
InactiveCN102298059AMicrobiological testing/measurementColor/spectral properties measurementsElisa kitEnzymatic Colorimetry
The present invention relates to the measuring method of the content of ammonia (ammonia ion) using enzyme cycle amplification method, enzyme colorimetric method and enzyme-linked method technology, the composition and the composition of reagent, the technical principle of its measuring is based on glutamic acid oxidase, glutamic acid oxidase, glutamic acid The serial catalytic reactions of alanine aminotransferase and alanine dehydrogenase are completed, and the invention also relates to an ammonia (ammonia ion) diagnosis / measurement kit. The assay method of the present invention has high sensitivity and small error, so the assay method and kit of the present invention can be widely used in clinical medicine / food inspection.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration
InactiveCN101324560AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsFerricytochrome cAlanine dehydrogenase
The invention relates to a kit for diagnosing / mensurating D-lactic acid by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA) based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, 2-ferricytochrome-c, ammonia ions, D-lactate dehydrogenase, alanine dehydrogenase, glycine oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the D-lactic acid.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
5' nucleotidase diagnosis reagent kit and 5' nucleotidase activity concentration determination method
InactiveCN101169376AMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementNucleotidaseAdenosine 5 monophosphate
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Inorganic phosphorus (phosphate radical) diagnosis/determination kit and method for determining inorganic phosphorus (phosphate radical) concentration
InactiveCN101620153AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsPhosphatePotassium
The invention relates to an inorganic phosphorus (phosphate radical) diagnosis / determination kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining inorganic phosphorus (phosphate radical) concentration and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food, and environment. The kit comprises the following main components: buffer solution, reduced coenzyme, adenosine diphosphate, oxaloacetic acid, ammonium chloride, potassium chloride, phosphoenolpyruvate carboxykinase, pyruvate kinase, pyruvate dehydrogenase and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the descending speed of absorbance at the position where the dominant wave length is 340nm to determine the inorganic phosphorus (phosphate radical) concentration.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Strain and method for producing hydroxytyrosol
PendingCN114574417AEasy to getGuaranteed to continueBacteriaTransferasesHydroxytyrosolEscherichia coli
The invention discloses a strain and a method for producing hydroxytyrosol, and belongs to the technical field of bioengineering. According to the present invention, the recombinant cell for expressing the omega-transaminase, the alanine dehydrogenase and the alcohol dehydrogenase is constructed, and the appropriate RBS strength is adjusted, such that the efficiency of the recombinant escherichia coli cell for catalyzing the dopamine to synthesize the hydroxytyrosol is improved, the yield of the hydroxytyrosol after the reaction for 24 h can achieve 146 g / L, and the good industrial application prospect is provided.
Owner:JIANGNAN UNIV
Magnesium (ion) diagnosis/measuring reagent kit and magnesium (ion) concentration determination method
InactiveCN101464365AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsEnzymatic ColorimetryHexokinase
The invention relates to a kit for diagnosing / measuring magnesium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of magnesium (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glucose, adenyl pyrophosphate, oxaloacetic acid, phosphate radical, ammonium chloride, hexokinase, pyruvate carboxylase, lactamine dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of magnesium (ions).
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Magnesium (ion) diagnosis/measuring reagent kit and magnesium (ion) concentration determination method
InactiveCN101464375AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsEnzymatic ColorimetryHexokinase
The invention relates to a kit for diagnosing / measuring magnesium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of magnesium (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glucose, adenyl pyrophosphate, enolphosphopyruvate, ammonium chloride, hexokinase, pyruvate kinase, lactamine dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of magnesium (ions).
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Genetically engineered bacterium for producing L-theanine by utilizing glucose de novo fermentation, method and application
The invention discloses a genetically engineered bacterium for producing L-theanine by utilizing glucose de novo fermentation, and the genetically engineered bacterium contains gamma-glutamyl methylamine synthetase for synthesizing theanine and a gene for coding a path enzyme for de novo synthesis of theanine. The theanine de novo synthetic pathway enzymes comprise phosphoketolyase, transaminase, acetaldehyde dehydrogenase and alanine dehydrogenase. A complete anabolism pathway from glucose to theanine is constructed, a genetic engineering strain which does not carry plasmids, does not need to add ethylamine and can stably produce theanine by using glucose is obtained, after the strain is fermented for 33 hours, the yield of theanine can reach 46 g / L, the conversion rate can reach 0.16 g theanine / g glucose, the production intensity can reach 1.4 g theanine / L / h, and the production cost can be greatly reduced. The highest yield, the conversion rate and the production intensity of the theanine produced by directly utilizing the glucose for de novo fermentation without adding ethylamine are reported at present, and the method has a good industrial application prospect.
Owner:TIANJIN UNIV OF SCI & TECH
Homocysteine diagnostic kit and method for measuring homocysteine concentration
InactiveCN101609013AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsLyaseWavelength
The invention relates to a homocysteine diagnostic kit utilizing technologies of an enzymatic recycling method, an ELISA multiplication method, an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring homocysteine concentration and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, reduced coenzyme, pyruvic acid, homocysteine-alpha, gamma-lyase, alanine dehydrogenase, glycine oxidase, NADH peroxidase, and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV / visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the homocysteine concentration.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Manganese diagnosis/measuring reagent kit and method for measuring manganese concentration
InactiveCN101173945AFast measurementImprove accuracyMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementOxaloacetate decarboxylaseManganese
The invention relates to a manganese diagnostic / testing reagent kit using enzymatic colorimetric method and enzymic linkage technology as well as method for testing concentration of manganese as well as composition and content of the reagent, belonging to technical field of medicine / food / environment test. The invention is characterized in that composition in the reagent kit comprises buffer solution, reduction coenzyme, oxaloacetic acid, ammonium, oxaloacetic acid decarboxylase, alanine dehydrogenase and stabilizing agent; the sample is mixed with the reagent according to specified ratio to generate a series enzymatic reactions, then the reactant is arranged under a ultraviolet / visible light analyzer to test the decrease speed of absorbance at wavelength of 340nm, thereby the manganese concentration can be tested. The invention has the advantages that the test results can be easily obtained using ultraviolet / visible light analyzer.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Manganese ion diagnosis/determination kit and method for determining manganese ion concentration
InactiveCN101620080AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsManganeseEnzymatic Colorimetry
The invention relates to a manganese ion diagnosis / determination kit using the technology of an enzyme multiplication method, an enzyme colorimetric method and an ELISA method, a method for determining manganese ion concentration and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food and environment. The kit comprises the following main components: buffer solution, reduced coenzyme, oxalic acid, acetyl coenzyme A, ammonium chloride, oxalate oxidase, pyruvate dehydrogenase, alanine dehydrogenase, and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the descending speed of absorbance at the position where the dominant wave length is 340nm to determine the concentration of manganese ion.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Phenylalanine dehydrogenase as well as preparation method and application thereof
ActiveCN111893098AMicroorganism based processesOxidoreductasesPhenylalanine dehydrogenaseOrganic solvent
The invention discloses phenylalanine dehydrogenase as well as a preparation method and application thereof, and relates to strain culture, enzyme expression, separation and purification, detection ofionic liquid tolerance and organic solvent system tolerance of enzymes and the like. According to the invention, high-stability phenylalanine dehydrogenase which is resistant to an organic solvent and an ionic liquid and is required by industrial biological catalysis application is obtained. The phenylalanine dehydrogenase has the characteristics of good stress resistance and high stability in anon-aqueous phase system, can effectively prolong the half-life period and improve the catalytic efficiency in industrial production, achieves the purpose of reducing the production cost, and has important industrial application value.
Owner:XIAMEN UNIV
Carbon dioxide diagnosis/determination reagent kit and method for determining carbon dioxide concentration
InactiveCN101324601AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsAlanine dehydrogenaseAbsorbance
The invention relates to a kit for diagnosing / mensurating carbon dioxide by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the carbon dioxide, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, pyruvate oxidase, alanine dehydrogenase, hydrogen peroxide, acetyl coenzyme A, ammonia ions and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, an enzymatic reactions occurs, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the carbon dioxide.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Method for determining amino acid (nitrogen) concentration and amino acid (nitrogen) diagnosis/determination reagent kit
InactiveCN101324587AMicrobiological testing/measurementColor/spectral properties measurementsPeroxidaseNitrogen
The invention relates to a kit for diagnosing / mensurating amino acid (nitrogen) by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the amino acid (nitrogen), and belongs to the technology field of medical / food / industrial / agricultural / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, pyruvic acid, amino acid oxidase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the amino acid (nitrogen) directly.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Homocysteine diagnosis kit and method for determining homocysteine concentration
InactiveCN101620172AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsLyaseElisa method
The invention relates to a homocysteine diagnosis kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining homocysteine concentration, and composition and components of a reagent, and belongs to the technical field of medical test and determination. The kit comprises the following main components: buffer solution, reduced coenzyme, pyruvic acid, homocysteine-alpha, gamma-lyase, alanine dehydrogenase and a stabilizer. The method for determining homocysteine concentration comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the descending speed of absorbance at the position where the dominant wave length is 340nm to determine the concentration of homocysteine.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Reagent (kit) for diagnosing/determining amino acid and method for determining concentration of amino acid
InactiveCN101750319AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsEnzymatic ColorimetryEnzyme catalysis
The invention relates to a reagent (kit) for diagnosing / determining amino acid by using an enzyme colorimetric method and an enzyme link method, and also discloses a method for determining the concentration of the amino acid, a composition and components of the reagent, belonging to the technical field of medicine / food / environmental test determination. The reagent (kit) comprises the main components: a buffer solution, a coenzyme, pyroracemic acid, an amino acid oxidase, a glycine oxidase, an alanine dehydrogenase and a stabilizing agent. A sample and the reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions, and reactants are placed under an ultraviolet / visible light analyzer for detecting the degree of absorbance rise at the dominant wavelength of 340 nm, thereby measuring and calculating the concentration of the amino acid.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Inorganic phosphorus diagnosis/ determination reagent kit and method for determining inorganic phosphorus concentration
InactiveCN101324611AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsAdenosine diphosphateAlanine dehydrogenase
The invention relates to a kit for diagnosing / mensurating inorganic phosphorus by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the inorganic phosphorus, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamine, adenosine diphosphate, pyruvic acid, glutamine synthetase, alanine dehydrogenase, glycine oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the inorganic phosphorus.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Adenosine deaminase diagnostic kit and method for measuring activity concentration of adenosine deaminase
InactiveCN101609009AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsWavelengthAlanine dehydrogenase
The invention relates to an adenosine deaminase diagnostic kit utilizing technologies of an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring activity concentration of adenosine deaminase and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science. The kit mainly comprises the following compositions: buffer solution, coenzyme, adenosine, pyruvic acid, hydrogen peroxide, glycine oxidase, alanine dehydrogenase, and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV / visible analyzer; and the descending speed of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the activity concentration of the adenosine deaminase.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Method for determining amino acid concentration and amino acid diagnosis/determination reagent kit
InactiveCN101324584AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsEnzyme catalysisAlanine dehydrogenase
The invention relates to a kit for diagnosing / mensurating amino acid by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the amino acid, and belongs to the technology field of medical / food / industrial / agricultural / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, pyruvic acid, amino acid oxidase, alanine dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the amino acid.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
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