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Method for extracting concealed acidophilic bacteria cohesive action-beta-hydroxy-butanoic acid ester

A technology of hydroxybutyrate and extraction method, which is applied in the field of extracting intracellular poly-β-hydroxybutyrate of hidden acidophilus, which can solve the serious degradation of PHB molecules and other problems

Inactive Publication Date: 2009-01-14
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The first two extraction methods are used more, and the sodium hypochlorite method in the chemical reagent method is the most commonly used method at present. Its advantages are simple and fast, but it is easy to cause serious degradation of PHB molecules

Method used

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  • Method for extracting concealed acidophilic bacteria cohesive action-beta-hydroxy-butanoic acid ester
  • Method for extracting concealed acidophilic bacteria cohesive action-beta-hydroxy-butanoic acid ester

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Embodiment 1

[0008] 1. Culture and collection of strains

[0009] Acidiphilium cryptum strain DX1-1 was cultured to the mid-logarithmic growth phase, and the bacterial cells were collected by centrifugation at 10,000 r / min for 10 min, and the bacterial cells were washed twice with sterile water (pH 3.0). Resuspend the precipitated sludge with 20 mL of sterile water.

[0010] 2. Ultrasonic crushing

[0011] Put the centrifuge tube containing the bacterial solution in a beaker with ice cubes in advance, and ultrasonically break it according to the following procedure: work for 2s, interval of 4s, work 10-30 times.

[0012] 3. Dispersed NaClO / Chloroform Extraction of PHB

[0013] Add 80 mL of sodium hypochlorite solution (pH10) / chloroform with a volume ratio of 2:1 to 1:2 to the liquid after ultrasonic crushing, shake it on a shaker at 30°C for 60 minutes, and centrifuge at 8000 r / m to divide it into three layers of sodium chlorate solution. The middle layer is not broken. Parietal cells a...

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Abstract

The invention relates to a method for abstracting poly-Beta-hydroxybutyrate in the acidiphilium cryptum bacteria cell. The method mainly comprises the steps of thallus gathering, ultrasonication, chloroform / sodium hypochlorite abstracting, and the deposition is obtained after centrifugation by filling the alcohol and drying. By adopting the invention, the abstracting rate of the PHB in the Acidiphilium cryptum bacteria cell can be up to 88 percent, which is increased by 12 percent in comparison with the method for abstracting sodium hypochlorite and chloroform solely.

Description

Technical field: [0001] The invention relates to a method for extracting poly-β-hydroxybutyrate (PHB) from Acidiphilium cryptum. Background technique: [0002] Poly-β-hydroxybutyrate (poly-β-hydroxybutyrate, PHB for short) is a high molecular polymer accumulated in bacterial cells, and is an important raw material for biodegradable plastics. Because it not only has the characteristics of general chemically synthesized plastics, but also has the characteristics of biodegradability, biocompatibility and the use of renewable resources for biosynthesis, it has been widely valued by the international bioengineering community. The extraction cost of PHB is an important factor affecting its large-scale industrial production, so researching and exploring the extraction method of PHB is an urgent problem to be solved at present. Shen Zhongyao et al. reported that the current extraction methods of PHB mainly include secondary chemical reagents, organic solvent methods, mechanical cru...

Claims

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Application Information

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IPC IPC(8): C08G63/89C08G63/06
Inventor 邱冠周徐爱玲夏金兰杨宇张帅李家洛张燕飞历丽
Owner CENT SOUTH UNIV
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