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Preparation method of composite bacteria fermentation bed

A technology of composite bacterial agent and fermentation bed, which is applied in the field of preparation of composite bacterial agent fermentation bed, and can solve the problems of bulky volume, environmental pollution, and low density.

Inactive Publication Date: 2009-02-04
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the remaining amount of straw in our country is increasing. Since these wastes are small in density, bulky in volume, and piled up in large quantities, destruction and disposal not only require a certain amount of manpower and material resources, but also pollute the environment. Therefore, it is necessary to explore ways to solve this problem. Effective Ways

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  • Preparation method of composite bacteria fermentation bed

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Experimental program
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Effect test

Embodiment 1

[0043] 1. Selection of fermentation strains and preparation of medium

[0044] Bacteria A——Candida tropicalis (ACCC2004), the medium is 10°Bx or 12°Bx fresh wort 1000mL, pH5.5-6.0.

[0045] Bacteria——Lactobacillus plantarum (AS1.557), the medium is glucose 20g, sodium acetate 5g, peptone 10g, sodium citrate 5g, beef extract 10g, dipotassium hydrogen phosphate 2g, yeast powder 5gMgSO 4 ·7H 2 O 0.58g, Tween-80 1ml, MnSO 4 4H 2 O0.25g, distilled water 1000ml, adjust the pH value to 6.2.

[0046] Bacteria C—Streptococcus thermophilus (IFFI06038), the medium is polypeptone 5g, plant peptone 5g, beef extract 5g, yeast extract 2.5g, disodium phosphate glycerol 19g, ascorbic acid 0.5g, MgSO 4 ·7H 2 O 0.25g, lactose 5g, pH7.1, distilled water 1000mL.

[0047] Bacteria D——Bacillus subtilis (AS 1.108), the medium is peptone 10g, beef extract 3g, NaCL 5g, distilled water 1000ml, pH7.4.

[0048] F bacterium——Bacillus subtilis (ACCC11062), the culture medium is peptone 10g, beef extr...

Embodiment 2

[0064] 1. Selection of fermentation strains and preparation of medium

[0065] Bacteria A——Candida tropicalis (ACCC2004), the medium is 10°Bx or 12°Bx fresh wort 1000mL, pH5.5-6.0.

[0066] Bacteria——Lactobacillus plantarum (AS1.557), the medium is glucose 20g, sodium acetate 5g, peptone 10g, sodium citrate 5g, beef extract 10g, dipotassium hydrogen phosphate 2g, yeast powder 5gMgSO 4 ·7H 2 O 0.58g, Tween-80 1ml, MnSO 4 4H 2 O0.25g, distilled water 1000ml, adjust the pH value to 6.2.

[0067] Bacteria C—Streptococcus thermophilus (IFFI06038), the medium is polypeptone 5g, plant peptone 5g, beef extract 5g, yeast extract 2.5g, disodium phosphate glycerol 19g, ascorbic acid 0.5g, MgSO 4 ·7H 2 O 0.25g, lactose 5g, pH7.1, distilled water 1000mL.

[0068] Bacteria D——Bacillus subtilis (AS 1.108), the medium is peptone 10g, beef extract 3g, NaCL 5g, distilled water 1000ml, pH7.4.

[0069] Bacteria E—Bacillus subtilis (ACCC11062), the culture medium is peptone 10g, beef extrac...

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Abstract

The invention provides a preparation method of a compound bacteria fermentation bed. Firstly, five production ferments of candida tropicalis, lactobacillus plantarum, streptococcus thermophilus, Bacillu subtilis AS 1.108 and Bacillu subtilis ACCC 11062 are prepared, the living bacterium of each production ferment is not less than 10<9 > / ml, afterward, the five production ferments of lactobacillus are freeze-dried. According to the weight proportion of the freeze-dried bacteria powder, 2-3 parts of candida tropicalis powder, 2-5 parts of lactobacillus plantarum powder, 2-4 parts of streptococcus thermophilus powder, 1-3 parts of Bacillu subtilis AS 1.108 powder and 3-5 parts of Bacillu subtilis ACCC 11062 powder are taken out, well mixed and vacuum-packaged thereafter, thereby obtaining the compound bacteria. After that, the culture medium of the fermentation bed is made from 1 percent glucose, 2 percent rice hull (uncrushed), 15 percent water and 82 percent kibbled straw. The compound bacteria is added into the culture medium with the weight proportion of adding 10 grams compound bacteria into every 100 kilograms culture medium after being dissolved by a little warm water to be well mixed, and then tiled and compacted with a thickness less than 15cm. The compound bacteria fermentation bed can be completely fermented in 5 days under natural conditions. The use of the production of the invention can reduce the smell of the pigsty and the proliferation possibility of flies and mosquitoes, thus ensuring that the pigs can grow healthily and providing people with safe pork while protecting the environment.

Description

(1) Technical field [0001] The invention relates to a method for preparing a composite bacterial agent fermentation bed for raising pigs, and belongs to the field of feed additives. (2) Background technology [0002] The annual growth rate of breeding scale and output value in our country exceeds 10%, but at the same time, the environmental pollution brought by the breeding industry is becoming more and more serious. A survey conducted by the State Environmental Protection Administration in 23 provinces and cities across the country found that 90% of the large-scale livestock and poultry farms in the country have not passed the environmental impact assessment, and 60% of the farms lack the necessary pollution prevention and control measures. Untreated livestock and poultry manure accumulates arbitrarily, and sewage manure is discharged randomly, which brings a series of environmental problems such as occupying and polluting farmland and water bodies, producing stench and cau...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/16C12N1/20C12N1/16C12R1/74C12R1/25C12R1/46C12R1/125A23K10/12A23K10/37A23K20/163
CPCY02P60/87
Inventor 程涛侯俊财唐远征
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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