Method for preparing enzyme electrode with MWCNTs-TiO2/Nafion composite medium

[0051] 1. MWCNTs-TiO 2 The preparation steps of the enzyme electrode of / Nafion composite medium are as follows:

[0052] (1) MWCNTs-TiO 2 Preparation of core-shell nanocomposites

[0053] MWCNTs multi-walled carbon nanotubes were reflux oxidized in concentrated nitric acid at 140 °C for 10 h. The acid-treated MWCNTs were dispersed in 1% sodium dodecyl sulfate solution, ultrasonicated for 3 hours to make sodium dodecyl sulfate adsorb on the surface of the MWCNTs, rinsed repeatedly, and dried. Add 100 μL of n-butyl titanate, 100 μL of water, and 5 mg of MWCNTs adsorbed with sodium dodecyl sulfate to 4 mL of absolute ethanol. After sonicating for 30 min, the suspension was transferred to beaker A (volume 10 mL). Inject 4mL of double distilled water into beaker B (volume 20mL), then place beaker A in beaker B, seal the device, place it in an oven and heat at 80°C for 3h. After the reaction, the centrifuged solid sample was repeatedly washed with absolute ethanol and double d...