Application of compound 4-o-methyl-benzenesulfonyl-2(3H)-benzoxazolone and pharmaceutically acceptable salt thereof in preparing non-steroidal anti-inflammatory and analgesic medicines

[0014] 1. Effect of compound MBB on pain threshold of hot plate in mice

[0015] 1.1 Experimental steps:

[0016] Kunming mice were placed in a water bath with a water temperature of 55°C, and the mice kicked or licked due to heat stimulation.

[0017] Response to rear foot or limb movement, which is used as an indicator of pain response. Select 48 female mice (choose the mouse that licks the feet within 5 seconds and 30 seconds on the hot plate, and the unqualified ones are eliminated), and they are randomly divided into 4 groups, 12 in each group, which are respectively physiological saline control group, the invention The compound MBB administration group (suspension prepared with 0.5% sodium carboxymethylcellulose) and the positive control group (chlorzoxazone) were intragastrically administered to rats (see Table 1 for intragastric administration dosage). Afterwards, the time from when the mouse was placed on the hot metal plate to when the pain response occurred was re...

[0032] 1. The effect of compound MBB on the writhing response of mice induced by acetic acid

[0033] 1.1 Experimental steps:

[0034] Kunming strain mice, weighing 20±2g, were randomly divided into normal saline control group, MBB administration group of the compound of the present invention and positive control group (chlorzoxazone). Each group was intragastrically administered once a day (see Table 3 for intragastric administration dosage) for 3 consecutive days. One hour after the last administration, each mouse was intraperitoneally injected with 0.2ml of 0.6% acetic acid, and then observed the torsion caused by acetic acid within 15 minutes. Body times.

[0035] 1.2 Calculation and statistical analysis of experimental results (Table 3):

[0036] Inhibition rate (%) = (writhing times of blank control group - writhing times of drug administration group) / writhing times of blank control group × 100%.

[0037] Table 3 The effect of compound MBB on the number of times of ...

[0085] 1. Effect of compound MBB on carrageenan-induced toe swelling in rats

[0086] 1.1 Experimental steps:

[0087] Take 40 rats, half male and half female, weighing 160-180g, and randomly divide them into five groups according to body weight, 8 rats in each group, respectively: solvent control group (i.e. model group); MBB effect group (including three doses) and Positive control group (chlorzoxazone). Rats in each group were given 0.5% sodium carboxymethylcellulose, compound MBB and positive drug every day. The animals were gavaged once a day (see Table 9 for the gavage dose) for 3 consecutive days. One hour after the last administration, the diameter of the normal toe of the left hind limb of the rats in each group was accurately measured with a vernier caliper. Make a mark around the ankle joint of the hindlimb, and then subcutaneously inject 0.15ml of 1% carrageen suspension in the toes of the left hindlimb of the rat to cause inflammation. Subsequently, eve...