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Method for identifying related species of oyster

A technology for species identification and oysters, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of distinguishing different species, difficult species identification, cumbersome process, etc., and achieves wide applicability, clear and intuitive results, and method simple and easy effects

Active Publication Date: 2013-09-11
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

At present, the most common way is to use the difference of mitochondrial COI gene and ITS sequence as the classification basis to distinguish different species, but the difference between these gene sequences is often SNP or In / del, and the change of sequence length between different species is not obvious , so it is difficult to distinguish different species by electrophoresis and other methods; sequencing can obtain detailed base information of this gene or sequence, but the sequencing experiment process is cumbersome and expensive, and requires the support of large instruments, which is difficult to achieve in a short time Accurate, fast and easy species identification

Method used

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  • Method for identifying related species of oyster
  • Method for identifying related species of oyster
  • Method for identifying related species of oyster

Examples

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Embodiment 1

[0025] Identification of oyster relatives in the genus Pyrex:

[0026] There are many species of oysters in the genus Colossus, and the appearance of oysters is greatly affected by the environment, so it is difficult to correctly distinguish different species by phenotype. In terms of species classification and identification, there used to be many controversies. Nowadays, it is gradually tending to identify different species by using the difference of biomarker sequences such as mitochondrial COI gene sequence among different species.

[0027] a. Acquisition of the basis sequence for species identification: The COI gene sequences of different closely related oyster species in the genus Pyramid that are relatively common in my country are used as the basis sequence for species identification.

[0028] b. Primer design: Select a sequence region that can clearly distinguish the target species in the above-mentioned oyster COI gene, and use Primer Premier 5 software to design PC...

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Abstract

The invention relates to a related specie identification technology, in particular to a method for identifying related species of an oyster. The method specifically comprises the following steps of: by using an oyster genome DNA (Deoxyribonucleic Acid) as a template, designing a primer according to a tag sequence of the oyster specie genome; carrying out PCR (Polymerase Chain Reaction) amplification on the primer; and distinguishing oyster species by using Tm value difference in a high-resolution melting curve of an amplification product. Through the adoption of the method provided by the invention, the species can be identified rapidly, economically, simply and conveniently; and compared with a tag sequence sequencing result, an identifying result of the method provided by the invention is 100% in accuracy.

Description

technical field [0001] The invention relates to a technology for identifying related species, in particular to a method for identifying related species of oysters. Background technique [0002] Oysters belong to the class Anatomybranch (or bivalves) in the phylum Mollusca. Oyster has high economic value and is an important aquaculture object in all countries in the world. It has become the economic animal with the largest aquaculture output in China and even in the world. In particular, the completion of the whole genome sequencing of oysters marks that oysters have entered the genome era, and related basic research and applied research such as molecular biology research, functional genetics research, and omics research of oysters have been carried out one after another. The distinction and identification of species is the basis of various research work. However, oyster species are very complex and their appearance is easily affected by the environment. There are also many ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 王家丰许飞李莉张国范
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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