Watermelon tissue culture seedling grafting method
A technique for tissue culture of seedlings and watermelons, applied in the fields of botanical equipment and methods, seed and rhizome treatment, horticulture, etc.
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[0029] Taking commercial seedless watermelon tissue-cultured seedlings as scions and gourd rootstocks as an example, scion hardening, rootstock preparation and grafting processes are all carried out in the greenhouse. The specific implementation:
[0030] A kind of grafting method of watermelon tissue culture seedling, its steps are:
[0031] A, rootstock preparation, rootstock seed is sowed in the nutrient bowl through germination, stock seedling age 15 days-20 days, two cotyledons are flat and have a true leaf of the same size as a five-cent coin for grafting;
[0032] B. Scion preparation, grow on the growth medium for 25-30 days and grow to 2 cm-3 cm tissue culture seedlings, open the cover and harden the seedlings in a solar greenhouse at 20°C-25°C for 2-4 days, select Tissue culture seedlings with consistent growth and 4 leaves are used as scions;
[0033] C, top-insertion grafting, when grafting, first wipe off the base of the first true leaf of the rootstock seedling ...
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