Bock greenbrier rhizome polysaccharide extract and preparation method thereof

[0039] The preparation of embodiment 1 King Kong vine polysaccharide extract

[0040] Weigh 100g of the medicinal material of King Kongteng, put it in a 2000ml round bottom flask, add 8 times the amount of 75% ethanol solution, soak overnight, heat and reflux in a water bath for extraction for 2 hours, filter, then add 8 times the amount of 75% ethanol for reflux extraction for 2 hours, filter Then add 8 times the amount of 75% ethanol for reflux extraction for 1 hour, filter, evaporate the residue to dry the solvent, then add 12 times the amount of water, heat and reflux in a water bath at 80°C for 2 hours, filter, and then add 12 times the amount of water , heat and reflux for extraction for 2 hours, repeat again, filter, combine the extracts, centrifuge, and concentrate the supernatant to 250ml under reduced pressure (that is, the concentration of the medicinal solution is 0.4g raw medicinal materials / ml).

[0041] Add 4 times the amount of 95% ethanol to the concentrated s...

[0272] The investigation of embodiment 5 degreasing treatment

[0273] Weigh the medicinal materials of the same quality respectively, add 10 times the amount of methanol, 80% ethanol, petroleum ether (60-90℃), acetone, n-hexane, petroleum ether (60-90℃)-acetone (1:1, V / V) and chloroform-methanol (2:1, V / V), heated and refluxed for 3 times, each time for 1.5h, filtered, dried the filter residue, and then added 10 times of water and heated to reflux Extract 3 times, 1.5h each time, filter, collect the extracts respectively, concentrate under reduced pressure to the same volume, add 4 times the volume of 95% ethanol, precipitate with alcohol for 24 hours, centrifuge, collect the precipitate, dry under reduced pressure at low temperature, and obtain each Sample powder, spare.

[0274] Weigh 16 mg of sample powder from each group, put it in a 50ml volumetric flask, add a certain amount of water, ultrasonicate for 30 minutes, dilute the volume with water to the mark, shake well, f...