Lactobacillus paracasei subsp. tolerans strain, screening method and use
A technology of tough subspecies and Lactobacillus is applied in the field of strain, screening and application of Lactobacillus paracasei tough subsp.
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Embodiment 1
[0023] (One) Lactobacillus paracasei of the present invention subspecies tough FX-6-1 ( Lactobacillus paracasei subsp. tolerans FX-6-1) Screening and isolation of strains:
[0024] Inoculate Tibetan kefir grains into pure milk, statically ferment for 24 hours at 30 ℃, mix the fermented curd, and dilute the liquid fermented milk with 0.9% sterile saline to 10 -6 ~10 -8 , Vortex and mix, pipette 0.1 mL diluted sample to MC solid medium and MRS solid culture plate surface and spread evenly. Seal culture at 30°C for 24-48h until a single colony grows. A single colony that produced a clear calcium circle was picked and purified again on an MRS agar plate to obtain a single colony strain. A total of 49 lactic acid bacteria were collected.
[0025] The isolated acid-producing single colony strain was inoculated into MRS liquid medium, and statically sealed overnight at 30°C for culture. Subsequently, the activated lactic acid bacteria solution was added to the pure milk according to t...
Embodiment 2
[0050] Lactobacillus paracasei subspecies tough FX-6-1 ( Lactobacillus paracasei subsp. tolerans FX-6-1) Strain fermentation antibacterial composition:
[0051] (1) Lactobacillus paracasei subspecies tough FX-6-1 ( Lactobacillus paracasei subsp. tolerans FX-6-1) Preparation of antibacterial composition produced by strain fermentation
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