The invention relates to a preparation method of a 
bacteriocin-producing 
Lactobacillus plantarum subsp. Plantarum Zhang-LL active bacterial preparation, and belongs to the field of microbe application. In the preparation method, by using a 
bacteriocin-producing 
Lactobacillus plantarum subsp. Plantarum Zhang-LL CGMCC No. 6936 
bacterial strain, the active bacterial preparation is prepared through activation, expanding culture, high-density 
fermentation, 
centrifugation, freeze-
drying protective agent addition, a prefreezing process, a freeze-
drying process and other processes. The preparation method comprises the following steps: firstly, 
rapeseed peptone is selected as a substituting 
nitrogen source, so that the culture condition of the 
lactobacillus plantarum is optimized while the cost of a culture medium is reduced; a 5L of full-automatic 
fermentation tank is used for performing the high-density 
fermentation, and under the optimized low-cost culture medium and the optimized culture condition, the 
viable count of fermented liquid can reach above 1010CFU / mL. In the preparation method, 
potato pulp is used as a matrix of a freeze-
drying protective agent, and an optimal combined formula of the freeze-drying protective agent is determined, so that the 
survival rate of the 
Lactobacillus plantarum subsp. Plantarum Zhang-LL reaches above 60% and the viable content can reach above 1011CFU / g. The prepared active bacterial preparation is low in 
raw material price and relatively high in viable content, can provide a good foundation for developing a novel 
feed additive for poultry, and can provide a significant economic value.