95 results about "International unit" patented technology

It includes the stages of grinding the lignocellulosic biomass to a size of 15-30 mm, subjecting the product obtained to steam explosion pre-treatment at a temperature of 190-230° C. for between 1 and 10 minutes in a reactor (2), collecting the pre-treated material in a cyclone (3) and separating the liquid and solid fractions by filtration in a filter press (9), introducing the solid fraction in a fermentation deposit (10), adding a cellulase at a concentration of 15 UFP per gram of cellulose and 12.6 International Units of β-glucosidase enzyme dissolved in citrate buffer pH 4.8, inoculating the fermentation deposit (10) with a culture of the heat-tolerant bacteria Kluyveromyces marxianus CECT 10875, obtained by chemical mutagenesis from strain DER-26 of Kluyveromyces marxianus and shaking the mixture for 72 hours at 42° C.

The invention discloses a breeding method of the loach, which can be used for improving the spawning expediting rate and the fertilization rate of the loach and the livability of the loach seedlings. The breeding method of the load comprises the steps of: a. respectively breeding the male loach and the female loach a half month before spawning expediting; b. adding a biological odinagogue into forage one week before spawning expediting, wherein the biological odinagogue specifically comprises 15-20g of Chinese angelica, ligusticum wallichii, folium artemisiae argyi, andrographis paniculata, rehmanniae vaporata and compound vitamins respectively, and the adding quantity is that 5-10g of the biological odinagogue is added into each kilogram of the forage, and continuously feeding the forage which is added with the biological odinagogue for 7 days; and c. making sets according to the proportion that the ratio of the female loach to the male loach is 1:1.5, and injecting 100-150 international units of HCG (human chorionic gonadotropin) into each loach. The breeding method has the beneficial effects that the female loach and the male loach are synchronously estrous after the breeding method is used, so that the spawning expediting rate and the fertilization rate of the loach are greatly improved. After a preferable mode of the breeding method is adopted, the cost can be reduced when the seedlings are bred, and the bait with an enough quantity can be timely provided, so that the livability of the loach seedling is guaranteed, and the large-scale production is realized.

It includes the stages of grinding the lignocellulosic biomass to a size of 15-30 mm, subjecting the product obtained to steam explosion pre-treatment at a temperature of 190-230 DEG C for between 1 and 10 minutes in a reactor (2), collecting the pre-treated material in a cyclone (3) and separating the liquid and solid fractions by filtration in a filter press (9), introducing the solid fraction in a fermentation deposit (10), adding a cellulase at a concentration of 15 UFP per gram of cellulose and 12.6 International Units of beta -glucosidase enzyme dissolved in citrate buffer pH 4.8, inoculating the fermentation deposit (10) with a culture of the heat-tolerant bacteria Kluyveromyces marxianus CECT 10875, obtained by chemical mutagenesis from strain DER-26 of Kluyveromyces marxianus and shaking the mixture for 72 hours at 42 DEG C.

The invention relates to an SFM (serum-free medium) for culturing MSCs (mesenchymal stem cells). Based on volume, the SFM comprises the following components: 10.2 grams per liter of alpha-MEM (alpha-minimum essential medium), 2.4 grams per liter of sodium bicarbonate, 1 to 5 millimoles of L-glutamine, 50 to 300 milligrams per liter of poloxamer 188, 2 to 8 grams per liter of recombinant human albumin, 10 to 20 milligrams per liter of recombinant human transferrin, 2 to 10 milligrams per liter of recombinant human insulin, 1 to 5 millimoles per liter of Hepes, 50 nanomoles of beta-mercaptoethanol, 0.1 to 1 milligram per liter of lipid, 1 to 5 milligrams per liter of trace element, 0.1 to 5 milligrams per liter of glutathione, 0.5 to 5 milligrams per liter of para-aminobenzoic acid, 1 to 50 nanograms per milliliter of hydrocortisone, 20 to 50 milligrams per liter of vitamin PP, 5 to 50 milligrams per liter of vitamin C, 2 to 10mu M of compound shown in a formula I, 5 to 20mu M of compound shown in a formula II, 10 to 20 nanograms per milliliter of progestin, 1 to 10 milligrams per liter of putrescine, 1 to 10 international units per liter of heparin, 1 to 10 nanograms per milliliter of EGF (epidermal growth factor), 1 to 10 nanograms per milliliter of b-FGF (b-fibroblast growth factor), 1 to 10 nanograms per milliliter of HGF (hepatocyte growth factor) and 1 to 10 nanograms per milliliter of VEGF (vascular endothelial growth factor). The SFM for culturing the MSCs is a BPS-SFM which has determinate chemical components and is free of animal-derived substances.

The invention belongs to the pesticide field and particularly relates to a synergic pesticidal composition with active ingredients to be the combinations of fipronil and a series of subspecies of Bacillus thuringiensis (Bt for short), wherein the Bacillus thuringiensis subspecies include six subspecies: Bacillus thuringiensis var.kurstaki, Bacillus thuringiensis var.aizawa, Bacillus thuringiensis var.tianmensis, B.thuringiensis var.israelensis, Bacillus thuringiensis subsp.aizawai and Bacillus thuringiensis subsp. kenyae. The main agent thereof includes the following active ingredients by the weight percentages as follows: fipronil 0.1%-80%, Bacillus thuringiensis 100-100000 IU (international unit) / mg, or 10-1000 billion spore / g, or 0.01-20% toxin protein, and the rest is an accessory ingredient. The pesticidal composition can be efficiently used for controlling agricultural pests such as rice leaf rollers, chilo suppressalis walkers, diamondback moths, prodenia lituras, corn borers, and so on, and has particular characteristics of high efficacy, long lasting period and safe usage, so that the pesticidal composition belongs to environment-friendly pesticides and can prolong the generation of resistance, reduce production cost and increase economic benefit.

The invention discloses a fine feed for rhizomys in a rearing period. The fine feed comprises the following raw materials in percentage by weight: 20%-42% of corn, 12%-18% of soybean meal, 30%-50% of rice bran, 10%-23% of bran, 1%-3% of fish meal, 2% of calcium hydrophosphate, 0.5% of stone dust, 1% of common salt and 1% of premix. One kilogram of premix provides 88 million IU (international units) of VA (vitamin A), 60,000 IU of VE (vitamin E), 325 million IU of VD (vitamin D), 310g of VK (vitamin K), 110g of VB (vitamin B), 220g of VB, 65g of VB, 1220g of VB, 50g of pantothenic acid, 10g of nicotinic acid, 800mg of folic acid, 10g of biotin, 30g of Lys (lysine), 60g of Met (methionine), 30g of potassium, sodium, zinc and the like, and 30g of VC (vitamin C); and the main nutritional levels of the obtained fine feed are that the digestible energy is 2.85-3.01 Mcal / kg, and crude protein is 15.52%-18.07%. The fine feed disclosed by the invention can meet the nutritional requirements of the rhizomys sinensis in the rearing period, has a reasonable formula, and can ensure the rapid growth, robust physique, low disease incidence and low mortality rate of the rhizomys.

The invention relates to a livestock semen freezing diluent as well as a preparation method and application of the livestock semen freezing diluent. The preparation method of the livestock semen freezing diluent comprises the following steps of: adding 2.71 grams of trihydroxymethyl aminomethane, 1,4 grams of citric acids, 1.0 gram of monosaccharides, 10 myriad IU (International Unit) of penicillium, 10 myriad IU of streptomycin and 0.29-6.97 grams of inositol compounds to a right amount of ultrapure water; optionally adding 5-20 milliliters of penetrability protectants; uniformly stirring; regulating a pH value to 6.8-7.2; adding 5-20 milliliters of fresh yolk subjected to inactivation treatment; uniformly mixing, and then fixing the volume to 100 milliliters; centrifugalizing at low temperature for 1 hour; and filtering supernate to prepare the livestock semen freezing diluent. Because the inositol compounds can effectively inhibit the generation of ice crystals, the livestock semen freezing diluent disclosed by the invention reduces the mechanical injury caused by the ice crystals on semens, achieves the survival rate of the defrozen semens at about 75%, the activity more than 50%, the acrosome integrality at about 60% and the plasmalemma integrality at about 50% and achieves the non-return rate more than 70% after artificial insemination; and the livestock semen freezing diluent has the advantages of no immune response generation, purity guarantee, low price, and the like by using the inositol compounds as the ice crystal inhibitors of the semen freezing diluent.

The invention discloses a method for preparing a freeze-dried human blood coagulation factor VIII. The method comprises the following process of: dissolving by taking water for injection, comprising 3-10 IU (International Unit) / ml of heparin, as a heparin sodium solution and cryoprecipitating; performing PEG (Polyethylene Glycol) precipitation and taking supernatant; performing centrifugal filtration; performing S / D (Solvent / Detergent) inactivation at the temperature of 24-26 DEG C; performing DEAE (Diethylaminoethyl) Sepharose Fast Flow chromatographic column balance, adsorption, washing andelution; performing molecular membrane ultrafiltration; preparing, removing bacteria, sub-packaging, freeze-drying and capping; and dry-heating at the temperature of 99.5-100.5 DEG C and inactivating. In the invention, the process method of combining the PEG precipitation and an ion exchange chromatography technology is adopted; the method is easy and convenient to operate; the F VIII active recovery rate is increased; miscellaneous proteins can be removed on a large scale; the product yield reaches over 60 percent; and the specific activity of the product reaches 5 IU / mg and is obviously greater than a value which is not less than 1 IU / mg stipulated in the pharmacopeia. Meanwhile, the PEG residue is 0.08g / L which is obviously less than the value which is less than or equal to 0.5 IU / mg stipulated in the pharmacopeia, so that Al<3+> residues in the final preparation of an Al(OH)3 gel method are avoided; the product has high purity and high safety; and the quality of the final product is obviously improved.

The invention discloses a liquid for preserving bagged frozen pig semen and a method for preserving the same. The preservation liquid consists of liquid A and liquid B, wherein the liquid A contains 1 to 10 grams of glucose, 1 to 10 grams of lactose, 1 to 8 grams of sodium glutamate, 1 to 8 grams of polyvinylpyrrolidone, 100 milliliters of distilled water, 100 to 200 thousand of international units of antibiotic, 0.02 to 0.1 gram of vitamin B12, 0.02 to 0.1 gram of caffeine sodium benzoate, and 10 to 30 milliliters of egg yolk; and the liquid B contains 50 to 100 milliliters of the liquid A, 2 to 10 milliliters of glycerol, and 2 to 10 milliliters of N-N-dimethylformamide. The method for preserving the semen comprises the following steps: concentrating the semen after sampling the sperm, and diluting and refrigerating the liquid A with the concentrated semen proportionally and isothermally; diluting the mixture by using the liquid B for the second time, and subpackaging the semen into bags; and preserving the bagged semen in liquid nitrogen. The method is effective in maintaining the vitality of the sperms, has simple and convenient use and low cost, and can meet the needs of artificial insemination of the frozen pig semen.

The embodiment of the invention relates to a parameter acquisition method, which comprises the steps of acquiring an aggregate maximum bit rate (AMBR) parameter of user equipment (UE) and acquiring the information of a service controlled by the AMBR. The embodiment of the invention also relates to a rate control method, which comprises the steps of: acquiring the AMBR parameter of the UE and controlling non-GRB service data rate on the basis of the acquired AMBR parameter. The parameter acquisition method and relevant equipment in the embodiment ensure that the authorized AMBR parameter is sent through an IU (International Unit) interface in a UMTS (Universal Mobile Telecommunications System) or a TD-SCDMA (Time Division-Synchronization Code Division Multiple Access) system, thereby being convenient for acquiring the AMBR parameter. Moreover, the rate control method and the relevant equipment of the embodiment realize the sharing of rate authorization by utilizing the AMBR parameter to control a non-GRB service data rate.

The invention relates to a neural stem cells medium and a method for performing human neural stem cells in-vitro long-term culture and amplification by using the neural stem cells medium. The neural stem cells medium comprises the following ingredients by weight proportion: 100-1000 micrograms of heparin sodium, 10-100 micrograms of vitamin E, 5-50 milligrams of insulin human recombinant, 0.5-5 milligrams of putrescine, 2-10 micrograms of sodium selenite, 2-10 milligrams of human transferrin, 2-10 micrograms of progestin, 300 milligrams of L-glutamine, 5.9 grams of 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid, 10-100 micrograms of recombinant human epidermal growth factors, 10-100 micrograms of recombinant human basic fibroblast growth factors, 20-200 milligrams of vitamin C glucoside and 40,000-400,000 IU (international unit) of gentamicin. By the neural stem cells medium, the technical problems that human neural stem cells are easy to differentiate when cultured in vitro and long-term culture and amplification are difficult to implement are solved.

A biphasic lipid vesicle composition for treating cervical displasia by intravaginal delivery. The composition includes a suspension of lipid-bilayer vesicles having entrapped therein, an oil-in-water emulsion, human interferon alpha-2b and L-methionine, the composition having an interferon alpha-2b specific activity of between about 1-10 MIU (million international units) per gram composition, and between 0.01 to 0.5 weight percent L-methionine. In the treatment method, the composition is administered at a dose of between about 1-20 MIU interferon alpha-2b, and this dose is administered at least 3 days / week, for a period of at least 4 weeks.

The invention relates to a wine and a preparation method thereof, and in particular relates to a wine containing an SOD (Superoxide Dismutase) component and a preparation method thereof. The preparation method of the wine containing the SOD component comprises the following steps of: (1) preparing phyllanthus emblica wine juice; (2) preparing phyllanthus emblica fermenting juice; (3) blending and disinfecting; and (4) bottling and packaging. The prepared wine per liter contains 800-1200 thousand international units of SOD. The original anthocyanin in percentage by weight in the wine is 0.01-0.0375%. The wine containing the SOD component prepared by the preparation method not only maintains taste of wine, but also increases various nutritional substances and SOD, and has the edible, beautifying and health-care functions.

The invention relates to composite calcium carbonate / vitamin D3 granules for children prepared from the raw materials, such as calcium carbonate / vitamin D3 and an adjuvant. The preparation is granules, each bag contains 1,250mg of calcium carbonate and vitamin D3 with an international unit of 200, and the adjuvant is selected from a diluent, a bonding agent, a wetting agent and a flavoring agent. The invention also relates to a preparation method of the composite calcium carbonate / vitamin D3 granules for children, a prescription and a preparation technology are feasible, so that the grannles are suitable for mass production; and the prepared composite calcium carbonate / vitamin D3 granules are good in granulation property, and have better degree of homogeneity and higher dissolution rate. The composite calcium carbonate / vitamin D3 granules for children disclosed by the invention are suitable for children, old people and the persons who have swallowing difficulties, especially suitable for children.

Disclosed is a natural hirudin ointment, wherein the formula contains natural hirudin and conventional ointment matrix, and each 100g of the ointment contains natural hirudin 100-200000 international units. The ointment can be used for treating thrombotic phlebitis, burns, skin cracks, skin ulcer, chilblain, acne, acne and hemorrhoid.

A biphasic lipid vesicle composition for treating cervical displasia by intravaginal delivery. The composition includes a suspension of lipid-bilayer vesicles having entrapped therein, an oil-in-water emulsion, human interferon alpha-2b and L-methionine, the composition having an interferon alpha-2b specific activity of between about 1-10 MIU (million international units) per gram composition, and between 0.01 to 0.5 weight percent L-methionine. In the treatment method, the composition is administered at a dose of between about 1-20 MIU interferon alpha-2b, and this dose is administered at least 3 days / week, for a period of at least 4 weeks.

The invention is concerned with the skin care compound medicine for scar restoration. It contents: metallothionein 50-200ppm, epidermal growth factor 5-60 thousands international unit / 30ml, one or more types of additional skin care liveness materials and carriers are acceptable by skin. The invention boots up the speed of skin restoring, reduces scar and mark of burns, scald.

The invention discloses a cultivation method of a selenium-enriched blueberry. The cultivation method comprises the following steps: (1) a selenium-enriched organic fertilizer is applied to a blueberry fruit tree before the tree blooms; (2) after the blueberry is picked, astragalus smicus is interplanted and harvested, the soil is cultivated; (3) organic selenium nutrient solution is sprayed when fruits are inflated. Selenium-enriched treatment is performed to the cultivated blueberry by adopting a foliar spray and root application method, and exogenous inorganic selenium can be changed into ecological organic selenium protein in an astragalus smicus interplanting process, thus the cultivation method is good for blueberry growth. The total selenium content of the fresh blueberry fruits is 0.048mg / kg, and the total selenium content of organic selenium reaches 85.6%; the zinc content is 2.4mg / kg; the anthocyanin content is 1548mg / kg, SOD (superoxide dismutase) in 100g fresh fruit includes 4.85 international units, and the VE (vitamin E) content is 86.4ug / kg.

The invention provides a high-sensitivity EBV DNA quantitative detection kit based on ddPCR and a using method thereof. The kit comprises DNA extraction buffer solution, PCR primers and probes, a PCR reaction solution and EBV positive and negative control. When the kit provided by the invention is used for detecting ddPCR, the influences of PCR equipment, standard substances and the like can be effectively eliminated, and further high-sensitivity absolute quantification is further realized. The EBV DNA quantitative detection kit provided by the invention is capable of performing high-sensitivity accurate quantification on EBV in serum and plasma through a ddPCR technology, and providing a reference basis for diagnosis and curative effect monitoring of EBV related diseases. According to the kit, a function relationship between the international unit and common unit of EBV DNA international standard substances is determined to be 1IU / ml=2.5Copy / ml, the international traceability of EBV DNA is realized, and the international comparability between the detection results and quantification unit is realized.

The invention discloses a compound living insecticide, including materials of the following volume portions of 10 to 20 portions of diflubenzuron; 0.01 to 0.1 portion of tryptophan; 1 to 2 portions of dispersant; 2 to 5 portions of glycerin; 72.9 to 86.99 portions of sterile water; the invention also includes clostera anachoreta granulosis virus, Bacillus thuringiensis fungus gemma and ENHANCIN increasing protein. The content of the clostera anachoreta granulosis virus is 0.5 to 2 billion per milliliter; the content of the Bacillus thuringiensis fungus gemma is 2 to 6 billion per milliliter; the content of the ENHANCIN increasing protein is 20 to 50 international units per milliliter. The compound living insecticide has the advantages of reasonable compatibility of medicines, convenient manufacture, broad desinsection spectrum and good desinsection effect. The invention overcomes the defects of wettable powder and emulsion; accessory ingredient is easy to disperse. The invention is more convenient to apply in fields and has no remnant and environment pollution, thus having remarkable superiority in actual application and being suitable for massive application on the forest industry.

The invention provides a duck egg yolk anti-freezing agent for cryopreservation of donkey semen and a preparation method thereof. Each 100 milliliters of anti-freezing agent contains the following components: 3 to 4 grams of Tris, 1 to 2.5 grams of glucose, 1.5 to 3.0 grams of citric acid, 5 to 10 milliliters of glycerol, 10 to 30 milliliters of yolk, 450 to 550 thousand international units (IU) of penicillin, 45 to 55 thousand IU of streptomycin, and the balance of distilled water. The duck egg yolk anti-freezing agent solves the problems of long reproductive cycle of female animals, low artificial insemination rate, complex production operation of frozen donkey semen and high difficulty in the prior art.

A method for preparing sturgeon caviar from eggs collected from live sturgeon is characterized in that the method comprises following steps: selecting sturgeon whose ovary develops to the end of IV stage, injecting luteinizing hormone-releasing hormone analogue, hocusing the sturgeon with water containing clove oil 80 to 120 ppm when the ovary is completely free, cutting the belly to collect eggs, sterilizing eggs at 0 to 4 DEG C, adding non-iodized salt to prickle the eggs, draining with a screen, sterilizing with UV ray, suturing the sturgeon, disinfecting with medical hydrogen peroxide solution, injecting gentamicin with 2,000 to 20,000 international units, and temporarily culturing in clean water tank. The method is simple, convenient and needs no complex equipment. The caviar can be made from sturgeons fished naturally or cultured sturgeons without killing the sturgeons. The survival sturgeons are reintroduced into the original water area, and the survive rate is ensured to above 80%, thus protecting resources, achieving sustainable use, improving the utilization efficiency of sturgeons, and maintaining the ecological balance.

The invention provides a nano vitamin D3 for producing feed and a preparation method thereof. Every 1kg of nano vitamin D3 powder comprises the following nutrients and the contents of the nutrients: 3500,000 IU (International Unit) / g and 900g of carrier. According to the preparation method of the nano vitamin D3, the grain size of vitamin is controlled to be within 100nm and nano vitamin of a new preparation form having nano effect is formed. Compared with the common vitamin D3, the nano vitamin provided by the invention has remarkably improved superior physical properties such as dissolubility, absorptivity, trophism and physiological and biochemical process in an organism.

The invention discloses a preparation method of bitter gourd dietary fibers, which comprises the following steps: (1) washing fresh bitter gourd, cutting the bitter gourd into segments, adding water of which the amount is 4-10 times of the bitter gourd, and pulping; (2) processing the slurry obtained in the step (1) with a colloid mill, and homogenizing at high pressure; (3) heating the slurry to80-100 DEG C and keeping for more than 30 minutes, and reducing the temperature to room temperature; (4) performing ultrasonic treatment on the slurry obtained in the step (3), adjusting the pH valueto 6.5-7.5, and performing enzymolysis by adding protease with specific activity of 1,000-20,000 international units into each kilogram of bitter gourd; (5) after the enzymolysis, adding amylase withspecific activity of 1,000-20,000 international units into each kilogram of bitter gourd; after the enzymolysis is finished, performing enzyme deactivation; and reducing temperature to room temperature; (6) filtering the enzymolysis liquid obtained in the step (5) to obtain a filtrate and filter residue; adding ethanol with the concentration of no less than 95% into the filtrate until the concentration is 60-85%; standing overnight; and centrifuging and filtering to obtain a precipitate; and (7) combining the filter residue and precipitate obtained in the step (6) to obtain the bitter gourd dietary fiber. Through the invention, the purity of the bitter gourd dietary fibers is over 95%, and the bitter gourd dietary fibers have a good effect in regulating blood sugar.

Compositions for increased antioxidant potency of natural vitamin E (d-alpha-tocopherol), comprising alpha-, beta-, delta-, and gamma-forms of tocopherols and tocotrienols are disclosed. All of these compositions provide 400 International Units (IU), based on one mg of d-alpha-tocopherol provides 1.49 IU. These compositions showed antioxidant activities superior to natural d-alpha-tocopherol. These compositions are designed to provide protection of the cell membrane lipid layer, and protection against heart disease, cancer, and eye disease.

The invention discloses menopausal gonadotropin with high specific activity as well as a preparation method and application thereof. The specific activity of the menopausal gonadotropin with high specific activity is not lower than 5000FSH international unit / mg protein. Meanwhile, the invention discloses a method for preparing the menopausal gonadotropin with high specific activity by using dye affinity chromatography.

The invention relates to a method for obtaining a leakage rate by using a static pressure raising method. A standard leak is connected with a vacuum container to be tested through an introduction valve, a vacuum system is started to pump the vacuum container to have stable pressure, the pressure change in each time section is measured, a vacuum container leakage rate Q is obtained through formula calculation, and the unit is Pa*m3 / s. The method has the advantages of simplicity and low needed resources, the vacuum container leakage expressed by an international unit system can be obtained in one time of pressure raising process, the method is rapid and convenient, the resources can be conveniently obtained in the field, and so the cost is low.

The invention provides a biological preparation for facilitating the growth of tomato plants and preventing and treating late blight of tomatoes and a preparation method and application thereof, which relates to the technical field of biological pesticide. The biological preparation of the invention consists of 50g of raw duck yolk, 100 milliliters of edible vegetable oil, 100 milliliters of clean water and 62.5 milliliters of 1000 international unit / microlitre of Agritol. The preparation process of the biological preparation of the invention is the mixing of the four ingredients, which is characterized by convenient preparation, economic and effective, low cost, safe and no pollution. The preventing and treating effect of the biological preparation of the invention is averagely enhanced by 32.72 to 36.03 percent compared with the ordinary chemical preparations and 129.35 to 434.57 percent compared with the Agritol. Compared with clean water, the height-increasing rate of the plants is 6.19 to 14.19 percent.

The invention discloses a method for preparing a freeze-dried human blood coagulation factor VIII. The method comprises the following process of: dissolving by taking water for injection, comprising 3-10 IU (International Unit) / ml of heparin, as a heparin sodium solution and cryoprecipitating; performing PEG (Polyethylene Glycol) precipitation and taking supernatant; performing centrifugal filtration; performing S / D (Solvent / Detergent) inactivation at the temperature of 24-26 DEG C; performing DEAE (Diethylaminoethyl) Sepharose Fast Flow chromatographic column balance, adsorption, washing andelution; performing molecular membrane ultrafiltration; preparing, removing bacteria, sub-packaging, freeze-drying and capping; and dry-heating at the temperature of 99.5-100.5 DEG C and inactivating. In the invention, the process method of combining the PEG precipitation and an ion exchange chromatography technology is adopted; the method is easy and convenient to operate; the F VIII active recovery rate is increased; miscellaneous proteins can be removed on a large scale; the product yield reaches over 60 percent; and the specific activity of the product reaches 5 IU / mg and is obviously greater than a value which is not less than 1 IU / mg stipulated in the pharmacopeia. Meanwhile, the PEG residue is 0.08g / L which is obviously less than the value which is less than or equal to 0.5 IU / mg stipulated in the pharmacopeia, so that Al<3+> residues in the final preparation of an Al(OH)3 gel method are avoided; the product has high purity and high safety; and the quality of the final product is obviously improved.

The invention discloses a composition, a preparation method thereof and use thereof. The composition consists of interleukin 2 and dexamethasone or salts thereof, wherein the content ratio of the interleukin 2 to the dexamethasone or the salts thereof is 200 to 500 thousand international units of interleukin 2: 0.05 to 0.3mg of dexamethasone or the salts thereof. The invention discloses the composition which can be used for preventing and curing allergic diseases and graft versus host diseases and can be further used to cure autoimmune diseases.