359 results about "Dismutase" patented technology

Single amino acid compounds and methods for upregulating expression of a gene encoding an antioxidative enzyme, such as superoxide dismutase or catalase, to counteract harmful oxidative effects of reactive oxygen species and other free radicals are described. The single amino acid compounds may be used in compositions and methods to treat or prevent diseases and conditions characterized by undesirable elevation of reactive oxygen species and other free radicals.

The invention discloses a platinum nano-material with the activity of four mimic enzymes and a preparation method thereof. The preparation method comprises the following steps: using citrate ions as a modifier, reducing chloroplatinic acid by utilizing sodium borohydride, and preparing the platinum nano-material modified by citric acid. The platinum nano-material is simply and quickly prepared, the particle size is small, and the dispersibility and stability of a water solution are high. The obtained platinum nano-material has the activity of four mimic enzymes at the same time, and comprises peroxidase, oxidase, catalase and superoxide dismutase. The activity of the four mimic enzymes of the platinum nano-material can be adjusted and controlled through the concentration of the citrate ions used in the preparation process.

The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plant Potentilla astrisanguinea Lodd. Var. orgyrophylla, said isozyme having the following characteristics, O2-. scavenging activity remains same before and after autoclaving; scavenges O2-. from sub-zero temperature of -20° C. to high temperature of +80° C.; O2-. scavenging activity at 25° C. for 30 days without adding any stabilizing agent such as polyols or sugars; O2-. scavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4° C. for at least 8 months; contamination free and infection free from any living micro- and / or macro-organism after autoclaving; possesses temperature optima at 0° C.; possesses a molecular weight of 33 kD under non-denaturating conditions; possesses a molecular weight of 36 kD under denaturating conditions; has clear peaks in UV range at 268 and 275 nm; has an enzyme turnover number of 19.53x104% per nmol per min at 0° C.; and requires Cu / Zn as a co-factor, method for the preparation of the purified isozyme of autoclavable superoxide dismutase and formulations containing the said autoclavable superoxide dismutase.

The present invention provides a high density fermentation and a purification process of a recombination high temperature resistance superoxide dismutase, the construction method of the invention includes: using gene coded for SOD in a thermophilic bacteria as a template, designing specific primer amplification target gene having restriction enzyme sites, after double digestion, connecting to plasmid vector pET28a after the same double digestion, constructing a recombinant plasmid, named for pSOD, transforming plasmid pSOD to competence escherichia coli BL21(DE3) by chemical transformation method, obtaining strain having high SOD yield after screening, completing the construction of SOD engineering bacteria; the fermentation process includes four steps of first order seed culture, secondary order feed culture, batch fermentation and induced expression, fermentation product SOD is finally obtained; the fermentation process realizes high level expression of SOD, the expression of the target protein is more than 60% of the bacterial protein total; SOD has excellent thermal stability and heat resistance, the expression product accounts for more than 60% of the whole proteins, and fully soluble protein, avoiding any trouble in the course of inclusion body renaturation; the purification process is simple, having high yield, lower cost, the final product SOD has high purification, high activity and strength stability.

The invention provides a SOD gene from animal and a method for cloning and expressing the SOD gene in colibacillus and yeast cells, wherein a nucleotide sequence of superoxide dismutase is shown as SEQ NO.1; an amino acid sequence of the superoxide dismutase is shown as SEQ NO.2; carriers of nucleotide molecules are colibacillus plasmids or yeast plasmids; cells of the nucleotide molecules are formed by carrier conversion; and cells of the nucleotide molecules of the superoxide dismutase contain colibacillus containing the nucleotide molecules or converted by the carriers or pichia yeast containing the nucleotide molecules or converted by the carriers. The invention can prepare recombinant production strains which can efficiently express and secrete Cu / Zn-SOD, realizes the production industrialization of the Cu / Zn-SOD, and achieves good pH stability, favorable thermal stability and Cu / Zn-SOD products with anti-protease hydrolyzation capacity.

The present disclosure relates to methods of treating a range of diseases or conditions. The methods involve administration of a superoxide dismutase mimetic.

Methods and pharmaceuticals for inhibiting or preventing metastasis formation in animals, including humans, having primary tumors, through the administration of phosphorothioates including their thiol and disulfide metabolites are disclosed. These compounds stimulate angiostatin levels, inhibit matrix metalloproteinases (MMPs), and stimulate manganese superoxidase dismutase (MnSOD). Phosphorothioates, of which amifostine is an example, can be administered as a combination therapy with traditional cancer therapies, including chemotherapy, radiotherapy, surgery, immunotherapy, hormone therapy and gene-therapy. Inhibition or prevention of metastasis by phosphorothioates is independent of tumor type, including adenocarcinomas and sarcomas.

A stabilized superoxide dismutase (SOD1) analogue, wherein the side chains of two amino acids on two different SOD1 monomers are connected is provided. A method of producing a stabilized superoxide disumutase (SOD1) analogue comprises reacting a first SOD1 monomer, a second SOD1 monomer, and a cross-linker.

The invention relates to a termite pheromone attractant and a termite trap-killing agent using the attractant, and belongs to the technical field of termite trap-killing agents. The termite pheromone attractant comprises the following components: hydroquinone, arbutin, oleic acid, glucose, cyclohexanhexol, mannose, 2,6-di-tert-butyl paracresol, amino acid, a biological active enzyme, salt, codlemone, gloeophyllum trabeum, ethanol, 2-phenoxyethanol, n-hexane, dismutase, poria cocos, coriolus versicolor, tall gastrodia tuber, edible fungus, lucid ganoderma, bee honey, maltose, fermented sugarcane powder, fermented straws and acetone. The termite pheromone attractant disclosed by the invention synchronously has the functions of a termite phagostimulating pheromone and a termite trace pheromone by adding a chemical pheromone into a termite trap-killing agent, and can be used for significantly increasing the eating amount of termites on the trap-killing agent and improving the trap-killing effects of the termite trap-killing agent; and meanwhile, the termite pheromone attractant has extreme sensitivity and high specificity.

The invention discloses a method detecting activity of plant superoxide dismutase, catalase and peroxidase, which comprises the steps of grinding a sample by using a liquid nitrogen method, extracting three enzymes simultaneously by adopting one buffer solution, detecting enzyme activity and the like. In the method, SOD, CAT and POD in plants can be simultaneously extracted by adopting one buffer solution, so that the method fulfills the aim of simply and quickly detecting the enzyme activity of the SOD, CAT and POD, simplifies operation, saves time and labor, reduces the using amount of samples and reagent and the like, and contributes to the realization of batch operation, and the detection result is similar to that of the conventional method. Furthermore, the sample is ground by adopting a liquid nitrogen grinding method before enzyme solution is extracted, so that the method has the advantages of high grinding speed, good cell crushing effect and high extraction efficiency.

The invention discloses a process for adding superoxide dismutase (SOD) in fruit wine making. By adding the SOD into various of fruit wine, free radicals in body can be cleared once. The process comprises the following steps: (1) culturing yeast strains at 30+ / -1 DEG C for 24-30 hours, and inoculating 0.1-1% of the vigorous strains growing for a logarithmic phase into a fermentation vat; (2) strictly dividing the fermentation into primary fermentation of 4-7 days and secondary fermentation of 15-25 days before entering an ageing phase, wherein the sugar control standard in fermentation is 14%; (3) strictly testing the active unit of the SOD not lower than 6,000u / L by a pyrogallol auto-oxidation macro-method in a blending and self-inspection process; and (4) adding the SOD into the fruit wine. By adopting the process disclosed by the invention, the prepared fruit wine can clear redundant free radicals so that the free radicals reach a dynamic balance in body and the diseases caused by cellular aging are prevented (slowed down).

The invention discloses a method for improving the fermentation productivity of carotenoid, which is realized by the following steps: rhodotorula mucilaginosa is used as fermentation strains of the carotenoid, and the rhodotorula mucilaginosa is inoculated into a culture medium according to the inoculum size of 2%-10%; a ferrous ion solvent is added into the culture medium, wherein the volume of addition of the ferrous ion solvent is that the molarity relative to the culture medium is between 0.05-0.5mmol / L, and the fermentation temperature is kept at 27-33 DEG C; after the fermentation lasts for 32-40 hours, the temperature is lowered to 20-25 DEG C for continuous fermentation; the fermentation lasts for 24-46 hours. By adding ferrous ions, the capability of beta-carotene for converting into the high antioxygen carotenoid is greatly improved, the insufficience of quantity of beta-carotene dioxygen dismutase and beta-carotene hydrogenase is made up; the beta-carotene is converted into the high antioxygen carotenoid; by the method, the fermentation yield is improved by 30-34%.

The invention provides a Fe3O4 nanozyme with improved catalytic activity, and a preparation method and application thereof. By doping metal elements in Fe3O4 nanozyme, the dismutase activity of peroxidase, catalase and / or superoxide can be improved; the metal element doped Fe3O4 nanozyme can be prepared in large quantities, has low cost, good stability at room temperature, good safety, and good economic value and application prospect.

The invention discloses a cultivation method for a fruit tree bearing fruit rich in superoxide dismutase. The cultivation method is a planting method which employs one or more microbial preparations to produce fruit rich in copper-zinc superoxide dismutase (Cu / Zn-SOD). Compared with the prior art, the cultivation method provided by the invention has the advantage that the content of SOD is greatly increased. By eating the fruit, the health conditions of people can be greatly improved; elimination of sub-health can be facilitated; immunity can be enhanced; excess superoxide anion free radicals in human bodies can be scavenged; and occurrence of a plurality of diseases can be prevented.

The invention provides a coproduction process for extracting various bioactivators from pig placenta, which comprises the following steps: taking the pig placenta (containing farrowing blood) as a raw material, utilizing components rich in the raw material such as immune globulin (Ig), immunocompetent peptide, superoxide dismutase (SOD), lipopolysaccharide, placental protein and the like, and applying modern biochemistry technology and biological integration technology such as salting in, salting out, chromatography, ultra-filtration and the like according to different structures, molecular weights, isoelectric points, physical and chemical properties, biological activities of the components to make the coproduction process suitable for industrial production. Therefore, in the same flow of the production process, the effective components in the pig placenta with market development value can be produced, namely five products of the immune globulin, the immunocompetent peptide, the superoxide dismutase, the lipopolysaccharide and the placental protein. The process combines the conventional and modern processes, produces various products through coproduction, and not only makes full use of placenta resources to change the waste into the treasure, does not discharge waste liquid and prevents the environment, but also can greatly reduce the production cost and improve the economic benefit.

The invention discloses an anti-oxidation active extract of citrus peels as well as an extraction process and application thereof. The active extract of the citrus peels mainly contains a citrus flavonoid substance and pectic polysaccharide. The extraction process of the active extract of the citrus peels comprises the steps of preprocessing raw materials, refluxing and extracting by a dipping method, decompressing and concentrating and drying in vacuum. The invention takes the in-vitro oxidation resistance of the extract to combine with the extraction yield as indexes, adopts a response surface method to optimize the extraction process, has reasonable technological conditions and good effect stability and is suitable for industrialized production. The active extract of the citrus peels, which is obtained by extraction, has an obvious effect to a model mouse with senility caused by D-galactose and can lower the content of the MDA (malondialdehyde) of the plasma, the liver and the brain obviously; the activity of the SOD (superoxide dismutase) of the plasma, the liver and the brain is obviously enhanced; the activity of the GSH-Px (glutathione peroxidase) of the plasma and the liver is obviously enhanced; and the invention can be used for preparing an anti-oxidation medicine, an anti-oxidation food or an anti-oxidation health-care product.

A processed food excellent for activating superoxidase dismutase is disclosed. The food contains 30 to 70% by weight of barley, wheat, rye or oats; 1 to 50% by weight of water soluble dietary fibers; 1 to 10% by weight of oligosaccharides; 0.5 to 5% by weight of powdered lactic acid bacteria and 5 to 15% by weight of powdered green tea. A method of making the food and a method for activating superoxide dismutase using the food is also disclosed.

The invention discloses a protein and amorphous metal organic framework compound and a preparation method thereof. The organic framework compound has a 2 nm-50 nm mesoporous structure. The preparationmethod comprises the following step of enabling a protein, zinc ions and an organic ligand to react in a solvent, wherein the organic ligand is a compound containing an imidazole group; the protein is one or a combination of several kinds of cytochrome C, cytochrome P450, horse radish peroxidase, alcohol dehydrogenase, lipase, acetylcholin esterase, laccase, a green fluorescent protein, glucose dehydrogenase, glucose oxidase, trypsin, bacillus subtilis protease, carbonic anhydrase, aldehyde ketone reductase, amylase, saccharase, superoxide dismutase, urease and catalase. The preparation method of the protein and amorphous metal organic framework compound provided by the invention is simple to operate and mild in condition, the obtained product is high in protein embedding rate and good inprotein stability, and the biologic al activity of the protein is reserved to a greater extent.

The system engineering for ecologically culturing live pig and earthworm and making comprehensive utilization mainly includes the following measures: rearing life pig in pig house in plastic transparent greenhouse, using grains as main feed, adding earthworm powder as auxiliary feed, feeding the pigs excrement and urine into underground methand-generating tank, making anerobic fermentation to extract methane gas, filling it into cylinder; feeding earthworm at another side of the greenhouse, using residues in the methane tank as feed for earthworm, making liquid machane and earthworm excrementinto high quality organic composite fertilizer, using earthworm to extract superoid dismutase and making resudual material into earthworm powder as high-quality feed.

A new isoform of manganese superoxide dismutase (MnSOD) and polynucleotides encoding it have been identified. This isoform, MnSOD E3(−), is a splice variant lacking exon 3 of the full length MnSOD. The polypeptide can be expressed using appropriate host cells. Modulation of either the expression of the polynucleotides of the activity of the polypeptide is also described. Furthermore, diagnostic and therapeutic methods have been developed as a consequence of the isolation of the polynucleotides and polypeptides.

Provided are novel human copper-zinc superoxide dismutase, also known as superoxide dismutase 1 or SOD1, specific antibodies as well as fragments, derivatives and variants thereof as well as methods related thereto. Assays, kits, and solid supports related to antibodies specific for SOD1 are also disclosed. The antibody, immunoglobulin chain(s), as well as binding fragments, derivatives and variants thereof can be used in pharmaceutical and diagnostic compositions for SOD1 targeted immunotherapy and diagnosis, respectively.

The invention belongs to the field of medicament synthesis, and relates to Danshensu derivatives with a structure in the following formula, a synthesis method thereof, and application thereof in pharmacy. The synthesis method adopts a chiral synthesis method to synthesize the Danshensu derivatives with stable structures in high yield, in particular derivatives of dextrorotatory Danshensu with high optical purity. Through experiments of animal models with hypoxic myocardial cells, results show that the Danshensu derivatives can reduce LDH leakage of the hypoxic myocardial cells, improve the activity of hyperoxide dismutase in the cells, inhibit generation of a product of lipid peroxidation, promote the expression of anti-apoptotic gene bcl-2 mRNA and inhibit the expression of pro-apoptosisgene bax mRNA and apoptotic gene caspase-3mRNA; and the results prove that the Danshensu derivatives have an effect of protecting hypoxic cardiac muscle and can be used for preparing medicaments for treating ischemic heart disease.

The invention discloses application of remedied domestic waste composts in adjusting the activities of antioxidant enzymes of lawn plants; wherein, the remedied waste composts refer to compost matrixes obtained by eluting the composts with water based on the weight ratio of 1:1-3; the activities of the antioxidant enzymes comprise superoxide dismutase, peroxide dismutase and catalase. The technical effects of the invention show that the method of planting lawn plants on the remedied waste composts not only can enhance the activities of the protective enzyme systems of the lawn plants but also can improve the capabilities of the lawn plants in resisting unfavorable growth environment obviously, therefore, the technology not only finds a way for scientifically and effectively planting environment-friendly lawns on the matrixes of the eluted compost, but also can achieve the purpose of reducing the environment pollution caused by the domestic waste composts and provide technical support for applying the remedied waste composts to the lawn planting system.

The invention discloses preparation and application of an Mxene-Mn3(PO4)2 composite electrode material. The preparation is characterized in that two-dimensional Ti3C2 nanosheets are synthesized from aTi3AlC2 ceramic material as a raw material, and ATP (adenosine triphosphate) is used as a template to induce synthesis of Mn3(PO4)2 nanoparticles. The Ti3C2 / Mn3(PO4)2 nanocomposite synthesized with the method integrates good conductivity of Ti3C2 and biological mimetic enzyme activity of Mn3(PO4)2, and the composite material has excellent conductivity and catalytic performance by complementary advantages of the two raw materials, and is the excellent electrode material for preparing superoxide dismutase. The composite material can be used for constructing biosensors and has excellent application prospects in the fields of medical diagnosis, medical research and the like.

The present invention discloses a blueberry enzyme and a preparation method thereof, which belongs to the field of enzyme preparation. The preparation method comprises: (1) blueberries are washed and cleaned, hot air dried at a temperature of 50-60 DEG C, and reserved for the next step; (2) black sugar is disinfected with ultraviolet for 5-10s; (3) the obtained blueberries in the step 1 are put into a sterile reactor, the obtained black sugar in the step 2 is added into the blueberries at a 17.5-18.5% mass of the blueberries, and a stabilizer is added into the blueberries with a mass being 0.5-1.4% that of the blueberries to obtain a mixture; (4) yeasts are added into the mixture obtained in the step 3; and (5) the mixture is sealed and fermented. The addition amount of sugar in the blueberry enzyme is low, which avoids a large amount of sugar content during drinking the enzyme. The blueberry enzyme is short in reaction time, anthocyanins and superoxide dismutase ingredients are retained, and total phenolic content is high.

The invention discloses a technology for extracting proteins and superoxide dismutase (SOD) from horseradish tree leaves. The technology comprises the following steps: smashing and pulping fresh horseradish tree leaves, and performing digestion, pressure filtering, isoelectric precipitation and centrifugation to obtain precipitates and supernate; performing modification protection, thermal denaturation reaction and freeze drying on the precipitates to obtain SOD freeze-dry powder; performing isoelectric precipitation, centrifugation and spray drying on the supernage to obtain leaf protein powder. By the adoption of the technical methods such as normal-temperature digestion, isoelectric precipitation, centrifugal separation, the original functional characteristics of the proteins and the activity of enzyme are retained to the maximum extent, and the yield of the proteins and that of active enzyme are greatly increased; the obtained leaf protein powder is bright in color, high in protein content and stable in quality; the obtained SOD is high in activity, and the practical value and the economical benefit are higher. The technology is easy to operate, low in production cost, safe, reliable and suitable for industrial production.

The invention relates to the biomedical field, and particularly relates to application of dihydromyricetin in preparation of a liver treatment and regeneration promotion medicine. Dihydromyricetin shows relatively high oxidation resistance and characteristic on promoting cell growth as a flavonoid compound. The test shows that dihydromyricetin can effectively relieve liver tissue necrosis caused by acute hepatic failure and can promote hepatocyte proliferation, so as to obviously raise the survival rate of a model mouse suffering from acute hepatic failure; dihydromyricetin also can obviously improve the activity of superoxide dismutase in tissue and serum, increase the content of serum protein, and lower down the levels of glutamic oxalacetic transaminase, glutamic-pyruvic transaminase and tumor necrosis factor alpha.

The invention relates to a process for producing wines, in particular to a method for producing a dismutase loquat wine. The method for producing the dismutase loquat wine is characterized by comprising the following steps: A) selecting fresh and ripe fruits, sterilizing the peel by utilizing a 1 / 5000 potassium hypermanganate solution, washing with clean water, artificially removing the kernels, juicing by utilizing a juicer, and transferring the juice into a fermentation jar; B) forming sufficient oxygen space because the juice accounts for about 1 / 3-1 / 2 of the volume of the jar after being transferred into the jar, adding a 3-11% white sugar solution dissolved in advance to be uniformly stirred, and then, inoculating a fresh Sacch Cervisiae culture solution, wherein the inoculation amount is 0.01-1%; storing the mixture for not more than 24h, and culturing for 96-168h at 33+ / -3 DEG C so that the mixture is abundantly multiplied under the aerobic condition; and when the amount of live bacteria reaches more than 100 millions / ml, fermenting after executing. The dismutase can be matched with the loquat wine to produce the dismutase loquat wine which has good flavor, is beneficial to the human body and has the advantages of fragment wine flavor and sour, sweet and delicious taste.