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191 results about "Thermal denaturation" patented technology
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Thermal denaturation of protein (by circular dichroism) This protocol is used to determine the stability of protein. The thermal denaturation of protein can be monitored by circular dichroism spectrum.
Integrated approach for generating multidomain protein therapeutics
InactiveUS8309690B2Good curative effectImprove development efficiencyAntibody ingredientsImmunoglobulinsThermal denaturationTherapeutic protein
Owner:MEDIMMUNE LLC
Vaginal remodeling device and methods
ActiveUS20070233191A1More accurate, deliberate, and visibleLevel of contacting pressure to be better controlledUltrasound therapyHeart defibrillatorsEpitheliumThermal denaturation
This invention relates generally to apparatus and methods for tightening tissue of the female genitalia by heating targeted connective tissue with radiant energy, while cooling the mucosal epithelial surface over the target tissue to protect it from the heat. Embodiments include a treatment tip that comprises both an energy delivery element and a cooling mechanism. As the treatment tip contacts the epithelial mucosa, the tip cools the mucosa by contact, and delivers energy thought the epithelium to the underlying tissue, thereby creating a reverse thermal gradient. The effect of the applied heat is to remodel genital tissue by tightening it. Such remodeling may include a tighter vagina and a tighter introitus. The tightening may be a consequence of thermal denaturation of collagen as well as a longer term healing response in the tissue that includes an increased deposition of collagen.
Owner:INMODE LTD
Integrated approach for generating multidomain protein therapeutics
InactiveUS20100105873A1Improves overall drug development efficiencyGood curative effectLibrary screeningAntibody ingredientsThermal denaturationTherapeutic protein
The invention provides method for therapeutic protein drug development that incorporates therapeutic and / or formulation and / or manufacturing considerations in the early screening process. The approach involves screening a plurality of different variants of a domain that have been determined to have the desired therapeutic property to identify one or more variants that have desired therapeutic and / or formulation characteristics, and constructing the full multidomain proteins using the identified domain variants. The present invention also provides a method for determining the shelf life of multidomain proteins in formulations. The method comprises determining a thermal denaturation and / or renaturation curve of a domain of the protein whose unfolding leads to aggregation of the protein in a solution. The method evaluates the shelf life of the multidomain protein based on the denaturation / renaturation curve. The invention also provides methods for engineering multidomain proteins to improve their therapeutic and / or formulation characteristics.
Owner:MEDIMMUNE LLC
Method for producing soybean polypeptide powder
InactiveCN102511648AAdvanced technologyIncrease productivityProtein foodstuffs working-upSolubilityNeutral protease
The invention relates to a method for processing soybean isolated protein by utilizing a biological enzyme technology. The method comprises the following steps of: during preparation, selecting high-quality soy protein isolate, and dissolving; performing thermal denaturation; adjusting the pH value to 8.0 to 9.0; adding composite hydrolase of alkali protease, neutral protease, papain and trypsin, and reacting for 2 to 3 hours; adjusting the pH value to 4.0 to 6.0; inactivating enzyme; when cooling to a temperature below 50 DEG C, adding filter aid enzyme; collecting filtrate to dissolve filter residues; adding composite hydrolase of acidic protease, neutral protease, papain and trypsin, and reacting for 1 to 2 hours; inactivating the enzyme; when cooling to temperature of below 50 DEG C, adding the filter aid enzyme; filtering by a paperboard; performing ultrafiltration; drying by microwaves under vacuum; and crushing and sieving. The soybean polypeptide powder prepared by the method can improve the solubility of albumen powder to a maximum limit and keep the color and luster of appearance, mouthfeel, flavors and the bioactivity of active ingredients, offers a good mouthfeel, is easy to absorb and can be widely applied to industry of dairy products, drinks and health-care products.
Owner:天津诺奥酶生产力促进有限公司
Vaginal remodeling device and methods
ActiveUS8961511B2More accurate, deliberate, and visibleLevel of contacting pressure to be better controlledUltrasound therapyHeart defibrillatorsEpitheliumThermal denaturation
This invention relates generally to apparatus and methods for tightening tissue of the female genitalia by heating targeted connective tissue with radiant energy, while cooling the mucosal epithelial surface over the target tissue to protect it from the heat. Embodiments include a treatment tip that comprises both an energy delivery element and a cooling mechanism. As the treatment tip contacts the epithelial mucosa, the tip cools the mucosa by contact, and delivers energy thought the epithelium to the underlying tissue, thereby creating a reverse thermal gradient. The effect of the applied heat is to remodel genital tissue by tightening it. Such remodeling may include a tighter vagina and a tighter introitus. The tightening may be a consequence of thermal denaturation of collagen as well as a longer term healing response in the tissue that includes an increased deposition of collagen.
Owner:INMODE LTD
Quantitative detection method for bovine alpha-lactalbumin
InactiveCN102590413AEasy to operateGuaranteed reproducibilityComponent separationRelative standard deviationReversed-Phase Liquid Chromatography
The invention relates to a quantitative detection method for thermal-denaturation and non-denaturation bovine alpha-lactalbumin in milk and milk products by applying an enzymolysis-liquid chromatography and mass spectrometry combination technology. The quantitative detection method comprises the steps as follows: taking a certain amount of milk or milk samples, dissolving and diluting the milk or milk samples in water to obtain solution with total protein content being about 1mg / mL; after volume metering, correctly sucking 500 mu L, adding an internal standard substance, reacting disulfide bond with dithiothreitol (DTT), alkylating to protect sulfydryl produced in reaction by iodoacetamide (IAA), and then conducting constant-temperature and constant-time enzymolysis with trypsin; and separating enzymolysis products by reversed phase liquid chromatography, conducting detection with a mass spectrum multiple reaction monitoring (MRM) manner, and calculating the result by an internal standard method. The quantitation limit of the method is 0.001g / 100g; when adding amount is 0.2, 1.7 and 5.0g / 100g, the recovery rate is 98.9-110.8% (n is equal to 6) and repeatability: RSD (Relative Standard Deviation) is smaller than 7.6%; and the quantitative detection method can be applicable to the quantitative detection of samples with different contents of bovine alpha-lactalbumin.
Owner:ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
Phytases, nucleic acids encoding them and methods for making and using them
ActiveUS20100136113A1Increase feeding valuePromote digestionImmobilised enzymesPowder deliveryThermal denaturationNucleotide
This invention relates to phytases, polynucleotides encoding them, uses of the polynucleotides and polypeptides of the invention, as well as the production and isolation of such polynucleotides and polypeptides. In particular, the invention provides polypeptides having phytase activity under high temperature conditions, and phytases that retain activity after exposure to high temperatures. The phytases of the invention can be thermotolerant and / or thermostable at low temperatures, in addition to higher temperatures. The phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be formulated as foods or feeds or supplements for either to, e.g., aid in the digestion of phytate. The foods or feeds of the invention can be in the form of pellets, liquids, powders and the like. In one aspect, phytases of the invention are stabile against thermal denaturation during pelleting; and this decreases the cost of the phytase product while maintaining in vivo efficacy and detection of activity in feed.
Owner:BASF ENZYMES
Artificial blood vessel bracket and preparation method thereof
ActiveCN101406713AImprove utilization efficiencyGood biocompatibilityStentsSurgeryThermal denaturationSide effect
Owner:SHANGHAI MICROPORT MEDICAL (GROUP) CO LTD
Intelligent tissue mimicking ultrasonic phantom and method of preparing the same
InactiveUS20100330545A1Resists deteriorationEnsure consistency of qualityUltrasound therapySurgeryThermal denaturationLower critical solution temperature
Owner:CHONGQING HAIFU MEDICAL TECH CO LTD
Heat-Transfer Resistance Based Analysis Bioparticles
ActiveUS20140011198A1Label-free and reliableBioreactor/fermenter combinationsBiological substance pretreatmentsThermal denaturationBiology
A method an system is disclosed for characterising DNA and / or RNA duplexes. The biosensing device comprises a heating element using a power and being suitable for providing thermal denaturation of target DNA and / or RNA bioparticles, a sample holder adapted for receiving a biocompatible substrate having a functionalized surface which is coated with probe DNA and / or RNA whereto target DNA and / or RNA duplexes can be attached, the sample holder further being adapted for exposing the biocompatible substrate at one side to the heating element, a first temperature sensing element for sensing a temperature at the side where the biocompatible substrate can be exposed to the heating element and a second temperature sensing element for sensing a temperature at the side opposite thereto with respect to the biocompatible substrate. The device also comprises a processing means programmed for calculating at least one heat transfer resistivity value based on temperature values obtained with the first temperature sensing element and the second temperature sensing element and the power for the heating element, for deriving a characteristic of the target DNA and / or RNA bioparticles from said heat transfer resistivity value.
Owner:INTERUNIVERSITAIR MICRO ELECTRONICS CENT (IMEC VZW) +1
Production method of direct-edible raw sea-slug
InactiveCN101356985AReduce lossesNo thermal denaturationFood preparationFish processingThermal denaturationAdditive ingredient
The invention relates to a preparation method of a raw sea cucumber which can be directly eaten. The preparation method is characterized in that internal organs are removed from a fresh sea cucumber, then the fresh sea cucumber is washed clean, and added with deionized water which is 0.5-10 times of the weight of the sea cucumber for ultrasonic processing at the temperature of 10-50 DEG C for 0.5-5 hours to obtain liquid; the obtained liquid is added with protease accounting for 0.1-5% of the weight of the sea cucumber at the temperature of 20-50 DEG C under the pressure of 0.01-0.5MPa for 0.5-10 hours to obtain solution; the sea cucumber is taken out from the obtained solution, the enzyme solution on the surface of the sea cucumber body is washed clean by clean water which is 1-5 times of the weight of the sea cucumber to obtain the raw sea cucumber which can be directly eaten. Sea cucumber protein prepared by the preparation method has no thermal denaturation, nutrient and beneficial ingredients such as sea cucumber mucopolysaccharide, sea cucumber saponin, and the like has little loss; collagen is softened but the sea cucumber does not shrink. The fresh sea cucumber has good mouth feeling and elasticity, and can be directly eaten.
Owner:OCEAN UNIV OF CHINA
Method and kit for rapidly constructing target nucleic acidsequencing library on basis of probe capture and enrichment
PendingCN107236729AImprove data qualityHigh-precision detectionMicrobiological testing/measurementLibrary creationGenomic SegmentThermal denaturation
The invention discloses a method and a kit for rapidly constructing a target nucleic acidsequencing library on the basis of probe capture and enrichment. The method comprises following steps and adopts reagents required for the steps: genome DNA is subjected to fragmentation; DNA end repair is performed; a probe and a single-stranded DNA fragment formed through thermal denaturation are subjected to molecular hybridization; biotin-avidinmagnetic bead separation is executed; the probe is taken as a primer for primer extension, and a complementary strand is synthesized; one end of a double-stranded DNA fragment is connected with an linker, and the other end is not connected with the linker; PCR amplification is performed; DNA fragment lengthselection is executed; the reagents are used for completing the reactions of all the steps. Compared withconventionaltarget nucleic acid enrichment technologies, the method has the advantages of good fidelity, good reproducibility, high speed and high universality.
Owner:上海阅尔基因技术有限公司
Preparation method of high-toughness polyphenyl thioether alloy
The invention discloses a preparation method of high-toughness polyphenyl thioether alloy. The preparation method comprises the following steps: adding an antioxidant, an ethylene-methyl acrylate-glycidyl methacrylate special ternary random copolymer toughening material and carbon black in a linear polyphenylene sulfide (PPS) resin subjected to oxidative thermal crosslinking, and then fully and evenly mixing in a high-speed mixer so as to obtain a PPS resin premix, wherein the weight percentage of PPS to glass fiber to special toughening material to carbon black to antioxidant is (59.8-65.2):(25-35):(3-8):(1-2):(0.2-0.8); and carrying out mixing, extrusion molding, cooling and chopping to obtain finished alloy. The alloy obtained by using the preparation method has the advantages of high thermal denaturation stability, high tensile strength and low metal corrosion rate, and can be widely applied to the fields of electronic appliances, machinery, chemical industry, petroleum, military industry and aerospace, especially used as an enclosure material or packaging material for all types of batteries.
Owner:SICHUAN DEYANG CHEM +1
Method for preparing stable albumin nano-particles by virtue of thermal denaturation
InactiveCN104490847AOrganic active ingredientsMacromolecular non-active ingredientsRedox responsiveDoxorubicin Hydrochloride
The invention belongs to the field of preparation of biological medical materials, and relates to a method for preparing stable albumin nano-particles by virtue of thermal denaturation. The method comprises the following steps: (1) adding vanillic aldehyde or an analogue thereof to form intermolecular disulfide bonds by virtue of inter-reaction of free sulfhydryl groups on albumin molecules under a heating condition; (2) enabling amino groups inside and among molecules to react with carboxyl so as to form amido bonds; and (3) enabling amino groups on the albumin molecules to react with aldehyde groups on vanillic aldehyde or the analogue thereof to form chemical bonds of Schiff base and the like so as to form stable nano-particles in an aqueous solution. Any organic solvent is not introduced during preparation, so that the prepared nano-particles are safe and nontoxic, and can well entrap antitumor drugs including paclitaxel, doxorubicin hydrochloride and the like. Moreover, the carrier has an oxidation reduction response in a tumor cell internal environment, and can open disulfide bonds to release drugs under the action of reducing glutathione in cells. The method provided by the invention is simple in process, convenient to operate and suitable for industrial mass production.
Owner:CHINA PHARM UNIV
Separation and purification method for recombinant hepatitis B core antigen
ActiveCN108047316AImprove thermal stabilityAggregate compact and stableVirus peptidesPeptide preparation methodsAntigenPurification methods
The invention relates to a separation and purification method for a recombinant hepatitis B core antigen. The method includes the steps of thermal denaturation and clarification; ammonium sulfate precipitation; ultrafiltration and concentration, washing filtering and liquid exchange; depolymerization; first-step molecular sieve chromatography; ultrafiltration and concentration, washing filtering and liquid exchange; repolymerization; second-step chromatography. By means of the method, the high-purity, low-host-residue and high-stability recombinant hepatitis B core antigen with a uniform granular structure can be obtained, that is to say, the method has the advantages that the antigen purity is high, the residue of host protein and host nucleic acid is low, antigen particles are uniform, and industrial enlargement is easy.
Owner:JIANGSU THERAVAC BIO PHARMA
Apparatus and method for assessing tissue treatment
ActiveUS20170071664A1Ultrasound therapyControlling energy of instrumentThermal denaturationAutomated algorithm
The invention relates to a tissue monitoring apparatus, a tissue monitoring method and an ablation lesion monitoring, measuring, and controlling automated algorithm incorporating diffuse reflectance spectroscopy (DRS) and / or Arrhenius model thermal denaturation kinetics for determining the characteristics of the lesion or the tissue, especially for identifying the transmurality of the ablation lesion. The invention pertains to a device for and method of real time monitoring of lesion formation as ablation is being carried out.
Owner:LIM BERNARD BOON CHYE
Method for raising thermal denaturation temperature of hide and hide powder through THP salt-nano clay combination tannage
InactiveCN101696456ATanning treatmentPre-tanning chemical treatmentThermal denaturationAlkalinizing agent
The invention discloses a method for raising thermal denaturation temperature of hide and hide powder through THP salt-nano clay combination tannage. The method comprises: adding softened hide or hide powder and water in a mass ratio of (0.5-1):1 to a reactor; adding a certain amount of salt and formic acid; adding THP salt and stirring for 3 to 5 hours; adding nano clay and stirring for 3 to 5 hours; extracting alkali till the pH of bath is between 5.0 and 6.0, continuing to stir for 2 hours and then standing the obtained product overnight; and stirring the obtained product for 30 minutes next day and then performing washing. The method can effectively raise the thermal denaturation temperature of hide collagen up to over 115 DEG C through the THP salt-nano clay combination tannage. Due to the specific structure of the nano clay added in the method, during alkali extraction in the later stage of tanning, tanning liquor is automatically alkalized and mitigated in effect, thereby helping to stabilize product quality, and the consumption of alkalization agent can be reduced. Meanwhile, the THP salt and the nano clay in the method containing no heavy metal salts are environment-friendly materials, and the tanning process is a clean leather-making technique.
Owner:SICHUAN UNIV
Evaluation method for degree of thermal denaturation of macromolecular collagen by using pepsin process
InactiveCN102621213AReflect comprehensive and intuitivePreparing sample for investigationMaterial analysis by electric/magnetic meansEthylic acidPepsin
The invention discloses an evaluation method for the degree of thermal denaturation of macromolecular collagen by using a pepsin process. The method is characterized by comprising the following steps: (1) selection of a raw material: a step of selecting leftovers of aquatic product processing-fishskin as the raw material; (2) immersion and dissolving: a step of adding acetic acid with a concentration of 0.01 to 1.0 mol / L into the raw material to obtain a collagen solution; (3) heat pretreatment: a step of heating the collagen solution, shaking the solution every five minutes during heating and carrying out rapid cooling after heating is finished; (4) enzymatic hydrolysis: a step of adding pepsin into the collagen solution, carrying out a heat reaction and adding phenylmethylsulfonyl fluoride after the heat reaction is finished so as to terminate the reaction; and (5) preparation of an electrophoresis sample and electrophoresis. The invention has the following advantages: used equipment is simple; more comprehensive and visual reflection of the denaturation state of protein is realized.
Owner:HUAIHAI INST OF TECH +1
Low-temperature exoinulinase mutant MutDL121EK5 with improved low-temperature adaptability and application thereof
ActiveCN112852782AChange thermal stabilityHigh activityBacteriaMicroorganism based processesBiotechnologyThermal denaturation
The invention relates to the technical field of gene engineering and protein modification, and discloses a low-temperature exoinulinase mutant MutDL121EK5 with improved low-temperature adaptability and application thereof, the amino acid sequence of the mutant MutDL121EK5 is obtained by replacing DAAPL from the 121st site to the 125th site of wild exoinulinase InuAMN8 with five amino acids EEDRK, and the sequence of the MutDL121EK5 is shown as SEQ ID NO.1. Compared with a wild enzyme InuAMN8, the mutant enzyme MutDL121EK5 has the advantages that the low-temperature activity is improved, the mutant enzyme MutDL121EK5 is more easily subjected to thermal denaturation, the improvement of the low-temperature activity is beneficial to reducing the dosage of the enzyme or shortening the reaction time during low-temperature reaction, and the easy thermal denaturation is beneficial to controlling the reaction process of the enzyme through thermal treatment. The low-temperature exoinulinase mutant MutDL121EK5 disclosed by the invention can be applied to the industries of food, wine brewing, washing and the like.
Owner:YUNNAN NORMAL UNIV
Method for extracting hemoglobin and superoxide dismutase from livestock blood
InactiveCN101353370AHigh technical requirementsGood repeatabilityPeptide preparation methodsEnzymesHuman bodyHemolysis
The invention discloses a method for extracting hemoy lobin and superoxide dismutase from bloods of livestock, which comprises the steps that: after going through an anti-freezing treatment, the fresh bloods of livestock is centrifuged to collect corpuscles, and after hemolysis, heating treatment is carried out so as to extract the hemoy lobin and SOD crude extracted liquid; SOD refined products are obtained after the SOD crude extracted liquid goes through treatments of thermal denaturation, super-filtration concentration, fractionated precipitation of acetone and absorption by ion exchanger. The method of the invention has simple operation and low production cost, is suitable for large-scale industrialized production, and can be used for simultaneously extracting the SOD and hemoy lobin in the bloods of livestock, thereby achieving the highly effective recovery of a plurality of protein resources from the blood of livestock. The extracted hemoy lobin contains salts such as sodium chloride and potassium chloride, etc which are harmless to the human body without containing copper ions. Therefore, the extracted hemoy lobin can be served as food for human beings and livestock, and also can be used for further producing hemoy lobin products such as hemachrome and decolored globin, etc.
Owner:抚顺丹阳生物科技股份有限公司
Process for producing soy milk and apparatus for producing soy milk
ActiveUS20100086660A1Avoid heatLimited functionMeat/fish preservationCheese manufactureThermal denaturationFood flavor
[Task] It is to provide a process and apparatus for producing soymilk which is obtained by completely deactivating various kinds of enzymes responsible for a grassy smell, while preventing heating unevenness, and inducing appropriate thermal denaturation of protein and a rich flavor, and which can be processed by coagulation into tofu high in water retentivity and elasticity and rich in flavor. [Solving means] The apparatus includes a submerged friction-grinding unit M having a friction-grinding portion in which soybeans are friction-ground together with soybean-grinding water to obtain a raw soybean soup, a heating unit N for heating the raw soybean soup obtained in the submerged friction-grinding unit M, a connecting pipe P2 for connecting the submerged grinding unit M and the heating unit N and a preheating unit Y1 attached to the connecting pipe P2 for preheating the raw soybean soup, whereby the raw soybean soup obtained in the submerged friction-grinding unit M is fed into the heating unit N while being preheated and is then further heated in the heating unit N The heating step by the heating unit N may be divided into multiple stages.
Owner:TAKAI TOFU & SOYMILK EQUIP
Albumen antioxidant peptide and preparation method thereof
ActiveCN102586376ALow antigenicityImprove functional propertiesPeptide preparation methodsFermentationUltrafiltrationFiltration
The invention discloses a preparation method of albumen antioxidant peptide. Egg albumen powder is used as main raw material, and the raw material is separated and purified through processes of thermal denaturation, enzymolysis,ultrafiltration, ion exchange, gel filtration, vacuum concentration and drying and the like to obtain the albumen antioxidant peptide with high activity. According to the invention, the albumen is subjected to enzymolysis with alkali protease, and albumen enzymolysis peptide with low molecular weight and high antioxidant activity is obtained through separation. In addition to reduction of antigenicity of albumen, improvement of functional characteristics and easiness for absorption, the product can also inhibit and eliminate free radicals as well as adjust and improve physiological functions, and can be applied to the research and development of medicines for preventing and treating chronic diseases. A new way for improving the additional value of the albumen, further processing eggs and application of eggs is developed, and the preparation method has wide market prospect.
Owner:DALIAN POLYTECHNIC UNIVERSITY
Apparatus and method for assessing tissue composition
ActiveUS9526426B1Maintain stabilityReduce stiffnessDiagnostics using spectroscopySurgical instrument detailsThermal denaturationAutomated algorithm
A tissue composition monitoring apparatus that includes a catheter with an optical sensor for tissue composition monitoring and ablation lesion determination via an automated algorithm incorporating Arrhenius model thermal denaturation kinetics for determining the characteristics of tissue for example the transmurality of the ablation lesion.
Owner:LIM BERNARD BOON CHYE
Phenylenediamine derivative, production method thereof and antioxidant for rubber using it as effective constituent
InactiveUS20050159519A1Easy to useMaintain good propertiesOrganic chemistryLayered productsThermal denaturationAlkoxy group
A phenylenediamine derivative represented by following formula (I) or (II): wherein R1 and R2 each represents a hydrogen atom, a lower alkyl group, or a lower alkoxy group; R3 represents a hydrogen atom or a methyl group; R4 represents a methyl group or an ethyl group; and n and m each is 1, 2, or 3; wherein R1 and R2 each represents a hydrogen atom, a lower alkyl group, a lower alkoxy group, or a cyano group; and n and m each is 1, 2, or 3, is disclosed. These phenylenediamine derivatives are effectively used as antioxidants for rubbers, which scarcely cause vanishing by evaporation or a thermal denaturation even under a high-temperature condition of 150° C. or higher.
Owner:NOK CORP
Isothermal amplication rapid detection method of H7N9 avian influenza virus
InactiveCN103305634AMicrobiological testing/measurementDNA/RNA fragmentationDirect observationAvian virus
The invention discloses a method for detecting H7N9 subtype avian influenza virus through loop-mediated isothermal amplification (LAMP). The method is used for detecting avian influenza virus in the fields of foods and raw materials, environmental samples, medical samples and health and disease prevention. According to the technical scheme, the method is characterized in that a specific primer is designed with regional gene sequences respectively coded as HA and NA in an avian influenza genome serving as target sequences, and a reaction system is optimized, so as to perform target gene specific amplification. The method only needs a constant-temperature device, does not need the thermal denaturation and long-term temperature cycle of a template, realizes the direct observation of the results, and has the characteristics of being low in cost, high in efficiency and simple in operation. The LAMP detection method of the H7N9 subtype avian influenza virus nucleic acid, created by the invention, has the characteristics of being high in specificity, high in sensitivity and convenient and quick, can be carried out in a grass-roots level or a small test site, and brings a novel technology and method for detecting the H7N9 subtype avian influenza virus.
Owner:ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Method for preparing corn protein foaming powder by enzymatic hydrolysis of com gluten meal
ActiveCN102630801AHigh in proteinGood foaming effectVegetable proteins working-upNeutral proteaseAlpha-amylase
The invention discloses a method for preparing corn protein foaming powder by enzymatic hydrolysis of com gluten meal. The method comprises the following steps of: pre-treating the com gluten meal by adopting enzymolysis of double enzymes, namely cellulase and alpha-amylase, centrifuging and washing to obtain corn protein concentrate; dissolving the corn protein concentrate into water, and performing thermal denaturation treatment at the temperature between 80 and 90 DEG C; adding sulfite or meta-disulfite for chemical modification; and adding neutral protease or alkali protease for hydrolysis, filtering, drying the clear solution, and thus obtaining a corn foaming protein product. The corn protein foaming powder prepared by pre-treating the com gluten meal by adopting enzymolysis of the double enzymes has high protein content, good foaming effect and high foaming stability; and the application range of the com gluten meal is further widened, the additional value of the deep processing products of the com gluten meal is improved, and the technological content and the quality of the deep processing products of the com gluten meal are greatly improved.
Owner:山东盛泰生物科技有限公司 +1
Manufacturing method of high-temperature resistant and corrosion-resistant polyphenyl thioether composite tube
The invention discloses a manufacturing method of a high-temperature resistant and corrosion-resistant polyphenyl thioether composite tube. The manufacturing method comprises the following steps of: adding fillers such as a thermal stabilizer, an inorganic filler and the like into polyphenyl thioether resin, and then mixing the materials fully and uniformly in a high-speed stirring machine to obtain a polyphenyl thioether resin premix compound; mixing and extruding the premix compound through a first-stage twin-screw extruder and introducing the premix compound into a single-screw extruder; extruding 3 to 6 layers of a fiber cloth formed by mix-twisting knitting a cylindrical basalt fiber and modified aramid fiber III after thermal expansion and contraction and a polyphenyl thioether composite fusant out of a tubular die on the head section of the extruder by the single-screw extruder; and cooling a pull tube into the polyphenyl thioether composite tube. The polyphenyl thioether composite tube manufactured by the method has the advantages of high thermal denaturation stability, corrosion resistance and high air tightness, and can be widely used in the fields of chemical industry, petrochemical industry, water supply and sewerage works, special industries and the like.
Owner:德阳科吉高新材料有限责任公司
Method for producing D-alpha-hydroxybutyric acid
ActiveCN101974603AShorten the growth cycleLow costMicroorganism based processesFermentationThermal denaturationHydroxybutyric acid
The invention discloses a method for producing D-alpha-hydroxybutyric acid, which comprises the following steps: (1) preparing complete cell suspension containing NAD independent hydroxy acid dehydrogenase or coarse enzyme liquid thereof with pseudomonas; (2) carrying out thermotropy on the complete cell suspension or the coarse enzyme liquid thereof prepared by the thermal denaturation step (1) to deactivate the NAD independent D-hydroxy acid dehydrogenase so as to obtain a biocatalyst only containing the NAD independent L-hydroxy acid dehydrogenase activity; (3) separating the racemic alpha-hydroxybutyric acid by the biocatalyst prepared in the step (2) to obtain the conversion fluid containing D-alpha-hydroxybutyric acid sodium salt and alpha-ketobutyric acid sodium salt; (4) pre-treating the conversion fluid; (5) acidifying the pre-treated conversion fluid; (6) separating the D-alpha-hydroxybutyric acid form the alpha ketobutyric acid; and (7) refining the D-alpha-hydroxybutyric acid. The method of the invention has the characteristics of simple components of culture medium, short growth period, low cost, easy subsequent separation and extraction, high enantiomeric excess of the product D-alpha-hydroxybutyric acid and the like and lays a foundation for efficient production of the D-alpha-hydroxybutyric acid.
Owner:上海肆芃科技有限公司
Hypoallergenic soybean peptide whole wheat flour as well as preparation method and application thereof
ActiveCN104782877AAllergenic protein content decreasedAvoid allergiesVegetable proteins working-upProtein solutionAlkaline protease
The invention discloses hypoallergenic soybean peptide whole wheat flour, as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing the soybean protein powder with water to obtain a soybean protein solution, and performing thermal denaturation on the soybean protein solution to obtain a denatured protein solution; (2) adjusting the pH value of the denatured protein solution to 6-9, adding neutral protease and performing first enzymolysis to obtain a first enzymatic hydrolysate; (3) adding alkaline protease and flavourzyme into the first enzymatic hydrolysate to perform second enzymolysis, and performing enzyme deactivation to obtain a second enzymatic hydrolysate; and (4) homogenizing the second enzymatic hydrolysate to obtain the hypoallergenic soybean peptide whole wheat flour. By adopting the preparation method, the sensitization of the soybean protein can be completely eliminated, the utilization rate of the protein is high and the mouth feel of the product is effectively guaranteed, and the prepared hypoallergenic soybean peptide whole wheat flour can be widely used in the milk powder or healthcare product.
Owner:CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
Method and appts of desolution of condensed soybean protein by alcohol process
InactiveCN1833525AShort heating timeSmall thermal denaturationVegetable proteins working-upThermal denaturationAlcohol
A method and apparatus for removing solvent from the concentrated soybean protein prepared by alcohol method in order to prevent its thermal denaturation is disclosed. Its advantages are simple operation, short time, and high effect.
Owner:中粮工科(西安)国际工程有限公司
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