A kind of primer and method for distinguishing larvae of A. lepidus and A. sylvaticus using est microsatellite markers

[0026] Chive tardigrades described in Example 1 were collected in 11 districts in Shandong Province in 2012;

[0027] In Example 2, A and B described in A and B were collected in 2012 respectively in Yiyuan District, Zibo City, Shandong Province and Changle District, Weifang City, Shandong Province;

[0028] A, B described in the embodiment 3 Chive tardigrade and Heterophthalmia were collected respectively in 2012 in Jining City, Shandong Province and Fei County, Linyi City, Shandong Province;

[0029] The above samples are all according to (Shi Baocai, Lu Hong, Gong Yajun, etc., 2010. Identification and control of Chive tardigrades. Chinese Vegetables, 11:21-22.; Zhang Hongrui, Zhang Xiaoyun, Shen Dengrong, etc., 2008. Edible fungi The biological characteristics of the fungus Bradysiadifformis. Chinese Edible Fungi, 27 (6): 54-56.) The above-mentioned Bradysiadifformis and different Bradysiadifformis were morphologically identified.

[0030] The instrument described in the embodiment is a Nikon SMZ745T stereo microscope, which is a common commercially available product.

[0031] Example 1

[0032] Constructed and sequenced the transcriptome library of T.

[0033] Firstly, the total RNA was extracted from the larvae of T. chileensis, and the extracted RNA was subjected to...