Valeriana Fauriei Briq. tissue culture breeding method

[0038] 1. Materials:

[0039] The test material, that is, the tissue culture explant is the young stem section of the northern valerian plant.

[0040] 2. Experimental steps:

[0041] (1) Explant induction: Rinse the tender stem section with tap water for 20 minutes, disinfect it with 70% ethanol for 1 minute, take it out after disinfecting it with 1% sodium hypochlorite solution for 15 minutes, rinse it 4 times with sterile water, and inoculate it at 1.0 mg / L 6-BA, 0.02mg / L IBA cultured on MS medium, the medium was added with 3% sucrose, 0.8% agar, pH 5.8, the culture temperature was 25°C, and the light time was 16h·d -1 , light 3000lux;

[0042](2) Subculture propagation culture: cut off the adventitious buds formed in step (1) under aseptic conditions, and inoculate the MS with a pH of 5.8 in 1.0mg / L6-BA, 0.02mg / L IBA and 8g / L agar. Proliferation experiments were carried out on the proliferation medium to obtain clustered shoots for subculture proliferation. The medium w...

[0056] 1. Materials:

[0057] The test material, that is, the tissue culture explant is the young stem section of the northern valerian plant.

[0058] 2. Experimental steps:

[0059] (1) Explant induction: Rinse the tender stems with tap water for 15 minutes, sterilize them with 60% ethanol for 30 seconds, sterilize them with 0.5% sodium hypochlorite solution for 10 minutes, take them out, rinse them three times with sterile water, and inoculate them at 0.5 mg / L 6-BA, 0.01mg / L IBA cultured on MS medium, the medium was added with 2% sucrose, 0.5% agar, pH 5.0, the culture temperature was 20°C, and the light time was 12h·d -1 , light 2500lux;

[0060] (2) Subculture proliferation culture: cut off the adventitious buds formed in step (1) under aseptic conditions, and inoculate in 0.5 mg / L 6-BA, 0.01 mg / L IBA and 8 g / L agar with a pH of 5.8 MS Proliferation experiments were carried out on the proliferation medium to obtain clustered shoots for subculture proliferation. The med...

[0074] 1. Materials:

[0075] The test material, that is, the tissue culture explant is the young stem section of the northern valerian plant.

[0076] 2. Experimental steps:

[0077] (1) Explant induction: Rinse the tender stems with tap water for 25 minutes, sterilize them with 80% ethanol for 90 seconds, take them out after sterilizing them with 1.5% sodium hypochlorite solution for 20 minutes, rinse them with sterile water 5 times, and inoculate them at 0.2 mg / L 6-BA, 0.01mg / L IBA cultured on MS medium, the medium was added with 5% sucrose, 2% agar, pH 6, the culture temperature was 30°C, and the light time was 20h·d -1 , light 4000lux;

[0078] (2) Subculture proliferation culture: cut off the adventitious buds formed in step (1) under aseptic conditions, and inoculate the MS with a pH of 5.8 in 0.2mg / L6-BA, 0.01mg / L IBA and 8g / L agar. Proliferation experiments were carried out on the proliferation medium to obtain the clustered shoots of subculture proliferation. The ...