Method for synergistic fermentation of soybean meal by employing bacillus subtilis and neutral protease

[0006] Specific embodiment 1: A method of synergistically fermenting soybean meal with Bacillus subtilis and neutral protease is realized by the following steps: 1. Take the Bacillus preserved in our laboratory from the glycerin tube, streak it on the LB plate, and cultivate it at 30°C After 24 h, when a single colony grows, pick a single colony and inoculate it on the slope of the test tube, culture it at 30 ℃ for 24 h, store it at 4 ℃, pick a loop of Bacillus subtilis and inoculate it into the seed culture medium at 37 ℃ and rotate at 120 r / Incubate for 16 h in a shaker for min. Separately take different milliliters of seed liquid, connect it to a triangular flask (500 mL) containing 100g of sterile mixed fermentation medium, culture for 36 hours, connect it to fermented soybean meal, and weigh a certain amount of enzyme into it. In the water, in a 40 ℃ water bath for 0.5 h, make the enzyme solution for enzymolysis, add soybean meal and mix well, put it into a fermentation b...

[0007] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, three levels of Bacillus subtilis inoculation are selected at 2.5%, 3%, and 3.5%, respectively, to measure the soybean meal peptide content after fermentation. The other components and steps are the same as the specific implementation method one.

[0008] Embodiment 3: This embodiment is different from Embodiment 1 in that the amount of neutral protease and enzyme added in step 1 is 250, 300, and 350 U / g, respectively, to measure the soybean meal peptide content after fermentation. The other components and steps are the same as the specific implementation method one.