1248 results about "Small peptide" patented technology

The invention provides a formulation for a special prandial food, which contains short peptides and is provided for tumor sufferers to eat. The food uses food-originated short peptides as raw materials which are added with extracts of medical-edible Chinese herbal medicines such as champignons, dried orange peels, Chinese wolfberry, yams, tuckahoes, rhizoma polygonati, and the like, and compound complex formulation. With the food formulation, powdered or granular foods can be prepared and are dissolved with warm water to eat. The food prepared according to the formulation is easy to dissolve, can be absorbed and utilized quickly, provides a high quality nitrogen source for organisms, supplements various vitamins and minerals necessary for a human body, can correct the negative nitrogen balance of the tumor sufferers, improve the nutritional status of the tumor sufferers, and can play a role in improving organism immunity and suppressing and resisting cancers through the combined action of various factors such as bioactive components, the functional oligosaccharides, and the like in the food-originated short peptides and the extracts of the medical-edible Chinese herbal medicines, thus the food is suitable for the tumor sufferers, particularly for cancer patients who receive radiation therapy and chemotherapy to eat.

The invention relates to a method for preparing health-benefiting fermented soybean meal enriched in small peptides, gamma-aminobutyric acid (GABA), reducing sugar, lactobacillus plantarum and antimicrobial peptide. By utilizing strains of aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum, the small peptides, free amino acids, reducing sugar, GABA and high viable count content in the lactobacillus plantarum in the health-benefiting fermented soybean meal are obtained through the two-stage solid state fermentation method, the small peptides account for 8.01-18 percent, the free amino acids has the content of 80-300mg / g, the reducing sugar reaches 20-80mg / g, the GABA reaches 0.317-0.5mg / g, the viable count content in the lactobacillus plantarum is 2-6.5*1010cfu / g, the antibacterial titer of antimicrobial peptides is 2.0*103-7.0*104 IU / g, and the yield of the health-benefiting fermented soybean meal is 82.03-90 percent. According to the process, the nutrient effect of the soybean meal can be improved, the prepared fermented soybean meal has the antibacterial effect, partial antibiotics can be replaced, the fermented soybean meal is conveniently preserved and saved, the process is simple and feasible, and the fermented soybean meal is green and environment-friendly and has application significance and wide prospect in the feed industry and breeding industry.

The invention provides a CP7 strain that can produce new antibacterial polypeptides that have strong antibacterial effect to gram-positive bacteria and are small peptides with 23 amino acid residues by the determination and whose amino acid sequence is: LLLAQPTAAVRGRCQVQRYLLA A. The invention also provides the application of the new antibacterial polypeptide in the preparation of the anti gram-positive bacteria drug.

A family of small peptides have been found to be inhibitory to TGF-beta activity, and preferably have the primary structure of Formula I:wherein AA1 is leucine, phenylalanine, alpha-aminoisobutric acid, N-methylalanine, N-methylisoleucine, or isoleucine; AA2 is the same or a different amino acid residue as in AA1; and AA3 is alanine or N-methylalanine.

The invention relates to a processing method of sea cucumber glycopeptides chelated calcium. The method comprises the following steps: A. material treatment: cleaning sea cucumber mouthparts, cooking, soaking, beating, and homogenizing; B. biological enzymatic hydrolysis: choosing a variety of protease for composite segmented enzymatic hydrolysis; C. centrifugal separation: conducting continuous centrifugal separation on an enzymolysis liquid to obtain the sea cucumber glycopeptides solution and sea cucumber calcium precipitate; D. filtration, concentration and grading: subjecting the enzyme liquid to ultrafiltration fractionation concentration, and desalinating small peptide filtered solution by nanofiltration; E. preparation of sea cucumber calcium by organic acidolysis; G. chelating reaction: promoting the chelating of sea cucumber calcium and glycopeptides of sea cucumber by microwave assistance; and H. after the reaction, carrying out centrifuging separation, and drying the precipitate and supernatant respectively. The present invention comprehensively utilizes the sea cucumber processing waste to prepare sea cucumber autologous calcium and autologous glycopeptides for chelation. The method on the one hand is beneficial to playing their biological effects, and on the other hand can also obtain five products with different specific biological functions.

This invention provides novel peptides that ameliorate one or more symptoms of atherosclerosis. The peptides are highly stable and readily administered via an oral route. The peptides are effective to stimulate the formation and cycling of pre-beta high density lipoprotein-like particles and / or to promote lipid transport and detoxification. This invention also provides a method of tracking a peptide in a mammal. In addition, the peptides inhibit osteoporosis. When administered with a statin, the peptides enhance the activity of the statin permitting the statin to be used at significantly lower dosages and / or cause the statins to be significantly more anti-inflammatory at any given dose.

The embodiments include methods of treating conditions requiring removal or destruction of cellular elements, such as benign or malignant tumors in treatment naïve mammals, using compounds based on small peptides. The method includes, but is not limited to, administering the compounds intramuscularly, orally, intravenously, intrathecally, intratumorally, intranasally, topically, transdermally, etc., either alone or conjugated to a carrier to a naïve mammal in need thereof who had not previously been treated for the condition.

This invention provides novel peptides for the treatment of atherosclerosis. In certain embodiments the peptide is X1-X2-X3-X4 where X1 and X4 are independently selected from the group consisting of alanine (Ala), valine (Val), leucine (Leu), isoleucine (Ile), proline (Pro), phenylalanine (Phe), tryptophan (Trp), methionine (Met), serine (Ser) bearing a hydrophobic protecting group, beta-naphthyl alanine, alpha-naphthyl alanine, norleucine, cyclohexylalanine, threonine (Thr) bearing a hydrophobic protecting group, tyrosine (Tyr) bearing a hydrophobic protecting group, lysine (Lys) bearing a hydrophobic protecting group, arginine (Arg) bearing a hydrophobic protecting group, ornithine (Orn) bearing a hydrophobic protecting group, aspartic acid (Asp) bearing a hydrophobic protecting group, cysteine (Cys) bearing a hydrophobic protecting group, and glutamic acid (Glu) bearing a hydrophobic protecting group; X2 and X3 are independently selected from the group consisting of Asp, Arg, and Glu; and the peptide converts pro-inflammatory HDL to anti-inflammatory HDL or makes anti-inflammatory HDL more anti-inflammatory.

The invention relates to a fermented liquid feed for pig and poultry as well as a preparation method and an application thereof, belonging to the field of biotechnology, in particular to a liquid feed produced by the fermentation of a plurality of beneficial microbes and Chinese medicinal herbs as well as a preparation method and an application thereof. The preparation method has simple process and comprises the following steps: after primary slant culture and secondary solution culture, lactobacillus, fermentation lactobacillus, bacillus subtilis, natto bacillus and saccharomyces cerevisiae are put into a solid fermenting tank with a fermentation medium containing astragalus root, Na2HPO4, nitrogen source and carbon according to weight proportion. The fermented liquid feed adopting the method has high quality, thicker mellow flavor and short fermenting time, contains protein, polypeptide, small peptide, amino acid, astragalus polyose and a plurality of beneficial microbes and is applicable to preparing various perfect compound feeds for pig and poultry.

The invention relates to the technical field of insect feed processing, in particular to a method for preparing protein insect feed with organic waste and the prepared insect feed. The method comprises the steps of directly crushing the organic waste into powder, mixing with beneficial microorganisms for fermentation, and adding necessary nutrition additives to form the insect feed. The raw material is not required to be sterilized and dried at high temperature; the production cycle is shortened; the production cost is lowered; simultaneously, macromolecular substances of the organic waste are converted to small peptides, organic acid and sugar by physiologic metabolism of the beneficial microorganisms; various nutrient substances such as bio-enzymes and vitamins which have a prebiotic effect on high protein insects and are easier to absorb are produced; and the purpose of recycling is achieved. Fermentation heat energy produced during fermentation of the microorganisms is effectively utilized to kill pathogenic microorganisms possibly existing in the organic waste, and the biosafety of the insect feed is ensured.

The invention relates to a preparation method of instant fresh water fish peptide powder. By using fresh water fish as raw materials, the preparation method comprises the following steps: removing fish skin and fish belly fat by a skin remover and a cube cutter, and then removing fishy smell and partial fat of the fish by using the technology of soaking with a mixture of CaCl2 and C2H4O2; then adding papain and Bacillus subtilis neutral proteinase for hydrolysis, carrying out inactivation and degreasing by the superhigh pressure technology, and separating slag from liquid by utilizing a centrifuge; and then adding activated carbon to the hydrolysate for bitterness and fishy smell removing treatments, filtering by utilizing a plate and frame filter press, concentrating the obtained filtrate in vacuum, and carrying out spray drying to obtain the instant fresh water fish peptide powder. The product prepared by the method has the characteristics of water solubility, creamy white color, palatefullness, no fishy smell and the like, and no organic reagent is used in the entire production process flow. The product is rich in small peptide, oligopeptide and various amino acids, can be used for manufacturing nutriment food for infants, advanced nutriment and health products for adults and the like, and also can be used as the ingredient for preparing advanced nutriment peptide coffee, flavorings and the like.

The invention relates to a method for preparing feed protein by mixed fermentation of tilapia leftovers and soybean meals, belonging to the field of feed additive preparation. The method comprises the following steps: mincing fresh tilapia leftovers, mixing the minced tilapia leftovers and crushed soybean meals at 60 meshes, adding water and uniformly mixing, and adding bacteria and enzyme to carry out solid fermentation; and drying and crushing the fermented raw materials to obtain the fermented feed protein. The fermented feed protein powder prepared by the invention is rich in small peptides and probiotics. According to the invention, the protein utilization rate is raised, the growth effect of animal is raised, the survival rate is raised. The fermented feed protein can completely replace fish meal in the feed industry, thus the feed cost is obviously reduced.

The present invention provides supramolecular hydrogels having a three-dimensional, self-assembling, elastic, network structure comprising non-polymeric, functional molecules and a liquid medium, whereby the functional molecules are noncovalently crosslinked. The functional molecules may be, for instance, anti-inflammatory molecules, antibiotics, metal chelators, anticancer agents, small peptides, surface-modified nanoparticles, or a combination thereof. Applications of the present invention include use of the supramolecular hydrogel, for instance, as a biomaterial for wound healing, tissue engineering, drug delivery, and drug / inhibitor screening.

The invention provides a multifunctional biological activity aquatic product feed additive, which can be prepared by following steps: (1) uniformly mixing material; (2) adding a little warm water of 30-50 DEG C into molasses and uniformly mixing, adding composite bacterium and uniformly mixing, placing for 1-2 hours, and adding residual water to prepare composite bacteria liquid; (3) adding the composite bacteria liquid into the material, uniformly mixing, and carrying natural aerobic fermentation for 50-60 hours at room temperature; (4) drying the mixture until moisture is less than or equal to 10% under temperature less than 60 DEG C, and grinding package. Content of the multifunctional biological activity aquatic product feed additive provided by the present invention is more than 10<12>cfu / g, the aquatic product feed additive contains a large amount of chitooligosaccharides, alga oligosaccharides and active small peptide, and simultaneously contains a plurality of protease, lipase and amylase. The invention has advantages of simple production method, no need of special heating sterilization and fermentation equipment, less energy consumption and short fermentation period.

The invention relates to a preparation method of feather albumen powder and the feather albumen powder prepared by the method. The method comprises the following steps: firstly carrying out pretreatment of washing, drying and cutting; then effectively carrying out steam explosion on feather by a steam explosion technique to damage the stable space structure of feather keratin; and finally carryingout zymohydrolysis treatment to enable the feather to become dissoluble small peptide and amino acid, thereby enhancing the digesting rate of pepsin of feather albumen and largely enhancing the biologic valence. The preparation method has the advantages of simplicity, low production cost, short processing period and high equipment efficiency. The content of crude albumen in the feather powder achieves more than 90%, and the digesting rate of in vitro pepsin achieves more than 88%.

The invention discloses a method for preparing fragrant peanut oil, which comprises the following steps: 1) a raw material is pretreated, wherein a peanut raw material is selected, is mixed with water, then is soaked, and then is subjected to coarse grinding and fine grinding; 2) complex enzyme is hydrolyzed, wherein the complex enzyme with neutral protease or alkali protease and flavor enzyme is selected to perform hydrolysis on the pretreated peanut raw material; 3) auxiliary materials are added, wherein reducing sugar, amino acid and peanut oil are added into a hydrolysate of the peanut raw material obtained through the treatment in step 2); 4) fragrance is generated through a thermal reaction, wherein the hydrolysate of the peanut raw material and the auxiliary materials are stirred evenly, then are transferred into a high pressure reaction kettle to be heated, and then are cooled to room temperature; and 5) the fragrant peanut oil is prepared, wherein a reaction liquid obtained after the treatment in step 4) is added with refined peanut oil, is quickly stirred first, and then is slowly stirred under certain temperature conditions, and water and impurities are removed through centrifugal separation to obtain the fragrant peanut oil product. The method can obtain flavor precursors of the fragrant peanut oil, namely amino acid and small peptide through the hydrolysis of the peanut raw material by the complex enzyme, and form the peanut oil with peanut fragrance flavor through the heating.

The invention belongs to the technical fields of feeds, enzyme preparations and microbial fermentation, and particularly relates to a preparation method of zymohydrolysis concentrated silage special for sows. The preparation method comprises the following steps of cutting short fresh pasturage or tuberous roots and stems, performing mashing, inoculating lactic acid bacteria, and performing natural enzymolysis and anaerobic fermentation so as to obtain silage; mixing one or more of cake meal, food processing by-products and cereals, with clean water and molasses syrup, inoculating lactic acid bacterium liquid, and performing natural enzymolysis and anaerobic fermentation so as to obtain fermented concentrated wet basis; and inoculating oxygen consumption fermentation bacterium liquid into a concentrated silage fermented substrate, and performing natural oxygen consumption fermentation so as to obtain the zymohydrolysis concentrated silage special for sows. The zymohydrolysis concentrated silage is rich in fermented cellulose lactic acid, calcium lactate, small peptide, amino acid, vitamins and probiotics, has the characteristics of being high in nutrition, easy to digest, good in palatability and the like, and can obviously increase the lactation quantity of the sows, obviously increase the food consumption and notably reduce the constipation of the sows.

The invention provides a formulation for a special prandial food, which contains short peptides and is provided for diabetics to eat. The food uses food-originated short peptides as raw materials which are added with extracts of medical-edible Chinese herbal medicines such as yams, mulberry leaves, kudzuvine roots, rhizoma polygonati, corn silks, tuckahoes, ebonies, polygonatums, and the like, and are supplemented with oligosaccharides, vitamins and minerals to form a compound formulation. With the food formulation, powdered or granular foods can be prepared and are dissolved with warm water to eat. The food prepared according to the formulation is easy to dissolve, can be absorbed and utilized quickly, provides a high quality nitrogen source for organisms, supplements various vitamins and minerals necessary for a human body, is advantageous to improve the nutritional status of the diabetics, and can play a role in reducing the blood sugar and controlling the blood sugar level of the diabetics, improving organism immunity and preventing diabetes complicating diseases through the combined action of various factors such as bioactive components, the oligosaccharides, and the like in the food-originated short peptides and the extracts of the medical-edible Chinese herbal medicines, thus the food is suitable for the diabetics to eat.

The invention provides a micro-ecological fermented protein feed and a preparation method, wherein the feed is prepared by mixing bean pulp and silkworms with quality ratio 90-98:2-10, then cooking to obtain solid state fermention medium, then inoculating mixed strains of bacillus subtilis, Saccharomyces cerevisiae, lactobacillus and Rhizopusoligosporus on solid fermention medium, then co-mixing for 30-45 hours at 33-37 DEG C, drying fermentation products after completing fermenting, crushing and obtaining the micro-ecological fermented protein feed. The inventive micro-ecological fermented protein feed contains a plurality of biological active peptides, content of small peptide reach more than 22%, which is favourable for digestion and development; contains lactobacillus, microzyme, which is favourable for controlling useful microbiological population in intestinal tracts of animals; has good delicious flavor and good luring effect, advances young animals for ingestion; contains soybean isoflavone more than 0.05%, markedly increases immunity of bodies, increases producibility of animals; relieving odmour of fecal waste; fully takes place of animal protein feed of fish meal and plasma protein flour and the like.

The invention relates to the deep processing of deep-sea cod, specifically a method for extracting collagen small peptide freeze-dried powder from deep-sea cod skin, comprising the following steps: 1) pre-treating deep-sea cod skin: soaking the fresh or frozen cod skin, decomposing into fluid for reservation; 2) biological enzymolysis; 3) separating and purifying protein small peptide; 4) freezing and drying to get the collagen small peptide freeze-dried powder extracted from deep-sea cod skin. The invention provides a wider use range for the biological protein in waste cod skin.

The invention provides a complex bacterium for bean pulp fermentation. The complex bacterium is composed of bacillus subtilis, bacillus coagulans, aspergillus niger, aspergillus oryzae, lactobacillus plantarum and saccharomyces cerevisiae. The complex bacterium provided by the invention is obtained through repeated scientific experiment and reasonable combination based on physiological metabolism feature of the microorganism, the complex bacterium is vigorous in growth metabolism, is capable of secreting a plurality of enzymes, acids and other metabolites, and is capable of efficiently decomposing macromolecule protein, anti-nutritional factors and sensitization factors in the bean pulp, obviously increasing content of soluble protein, small peptide, organic acid, protease and vitamin and the number of probiotics and antibacterial peptide, improving the digestion utilization rate, inhibiting the growth of pathogenic bacteria and reducing the use of antibiotic; and meanwhile, the complex bacterium has acid fragrance of fermentation and is good in food attractant effect. The bacteria strains forming the complex bacterium are included in a feed additive variety catalogue (2013) issued by the department of agriculture, the complex bacterium is safe and reliable, and can be directly used for the industrial production for the bean pulp fermentation.

The invention relates to protein feed rich in small peptides and multi-enzyme strains and a preparation method thereof. The protein feed is prepared by fermenting soybean meal, cottonseed meal, rapeseed meal, bone meal, wheat bran, common salt, light calcium carbonate, water and strains in a fermentation bin. The preparation method of the protein feed comprises the following steps: mixing the soybean meal, the cottonseed meal, the rapeseed meal, the bone meal, the wheat bran, the common salt and the light calcium carbonate in percentage by weight; adding 20-30 percent of water; sending the mixture to an air steamer; adding high-pressure steam to the air steamer so as to increase the air pressure to 1-2 MPa and the temperature to 140-200 DEG C; keeping the pressure for 4-6 minutes; momentarily releasing the mixture in the air steamer; sending the mixture to the fermentation bin; inoculating 3-5 percent of strains when the temperature of the mixture falls to 30-37 DEG C; fermenting for 72 hours at the temperature of 32-37 DEG C; discharging from the bin; and bagging to obtain a finished product. The product has strong disease-resistant function and low cost, increases the productionof animal by-products by more than 20 percent, is nontoxic, harmless and pollution-free and can inhibit food-borne diseases represented in cancers and subhealth phenomena from spreading.

The invention discloses a method for preparing functionality feed protein intestinal membrane albumen powder, which solves the problems that simultaneous hydrolyzing of multienzyme can affect the hydrolyzing degree and the acquiring rate of the protein peptide, more particularly, the lower acquiring rate of small peptide in the existing intestinal membrane albumen powder preparing technique. The technical scheme includes (1) treating before materials; (2) adding protease to hydrolyze; (3) adding a carrier; (4) preparing through sponging drying; wherein, in the step of hydrolyze the protease, hydrolyzing is carried through on any two of neutral protease, pawpaw protease and alkali protease by adopting a multienzyme grading hydrolyzing method. The functionality feed protein intestinal membrane albumen powder prepared by the method has the obvious advantages in the aspects of the acquiring rate of the protein peptide and the acquiring rate of the small peptide. The invention mixed to feed with other feeds can remarkably improve the performance trait of immature animals.

The present invention provides branched water-soluble polymers that allow two or more water-soluble polymers to be conjugated to another species. The branched polymers provide access to therapeutic agents that are conjugated at a single site to two or more water-soluble polymers. The branched polymers are based upon branch points that are simple branched alkyl structures, reactive side-chain amino acids and small peptides of reactive side-chain amino acids, and saccharides. Also provided is a method for preparing mono-disperse poly(ethylene glycol) of a well-defined and determinable molecular weight, and a method for the rational end-functionalization of poly(ethylene glycol). Conjugates of the branched water-soluble polymers with diverse species, e.g., peptides, lipids, glycolipids and small molecules are also provided.

The embodiments include methods of treating conditions requiring removal or destruction of cellular elements, such as benign or malignant tumors using compounds based on small peptides in combination with additional active agent(s). The method includes, but is not limited to, administering the compounds intramuscularly, orally, intravenously, intrathecally, intratumorally, intranasally, topically, transdermally, etc., either alone or conjugated to a carrier to a mammal in need thereof. Carrying out the method unexpectedly reduces the need for subsequent invasive surgical intervention.

The invention provides a small peptide additive for porcine feed and a preparation method thereof; the small peptide additive for porcine feed is characterized in that the additive is prepared by mixing base materials, saccharomyces cerevisiae liquid, lactobacillus acidophilus liquid, bacillus subtilis liquid and water according to a weight ratio of 100:3-4:3-4:5-7:35-4, and performing solid fermentation; the base materials comprise 50-70 wt% of soybean meal, 4-5 wt% of rapeseed meal, 10-15 wt% of fish meal, 5-8 wt% of vinasse, 2-5 wt% of corn starch, 1-3 wt% of wheat bran, 1-3 wt% of molasses, 1-3 wt% of yellow mealworm, and 0.5-1 wt% of sodium carbonate. The small peptide additive for porcine feed of the invention can increase the synthesis speed of protein for pig body, promote food ingestion of pigs, increase appetite, improve immunity, promote the absorption of mineral elements by pig body, regulate various physiological actions of the body, reduce stress, and improve the nutritional status of pig body.

The invention belongs to the technical fields of feeds, enzyme preparations and microorganisms, and particularly relates to a preparation method of a feeding-attractive glucolytic molasses feed. The preparation method includes following steps: mixing feed raw materials, feed-use microorganisms, a feed-use composite enzyme, molasses and water according to proportion, performing fermentation enzymolysis to prepare a glucolytic feed wet base material, mixing the glucolytic feed wet base material with molasses to prepare a glucolytic molasses feed wet base material, and finally drying the glucolytic molasses feed wet base material to prepare the feeding-attractive glucolytic molasses feed. The feeding-attractive glucolytic molasses feed include certain amounts of molasses, small peptides, lactic acid, yeast protein and probiotics and such small-molecular nutrients, and is good in palatability and high in digestion rate.

The embodiments include methods of treating conditions requiring removal or destruction of cellular elements, such as benign or malignant tumors using compounds based on small peptides in combination with additional active agent(s). The method includes, but is not limited to, administering the compounds intramuscularly, orally, intravenously, intrathecally, intraprostatically, intratumorally, intranasally, topically, transdermally, etc., either alone or conjugated to a carrier to a mammal in need thereof.

The present invention provides a highly sensitive screening assay for the identification of peptide binding partners to virtually any peptide or polypeptide ligand. Utilizing an expression-repression readout system, the inventors have screened libraries of peptides and identified relatively small peptide molecules that bind to the provided target.